Detailed information NTG0055578

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: HALHA_RS08770
• Genome accession: NC_019978
• Coordinates: 1821313..1822356 (-)
• Length: 347 a.a.
• NCBI ID: WP_015327442.1
• Uniprot ID: L0K9N2
• Organism: Halobacteroides halobius DSM 5150
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1816313..1827356

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HALHA_RS08740 (Halha_1784)	-	1816940..1817956 (-)	1017	WP_015327436.1	pilus assembly PilX N-terminal domain-containing protein	-
HALHA_RS08745 (Halha_1785)	-	1817958..1818470 (-)	513	WP_041607761.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
HALHA_RS08750 (Halha_1786)	-	1818476..1818847 (-)	372	WP_015327438.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
HALHA_RS08755 (Halha_1787)	-	1818866..1819600 (-)	735	WP_015327439.1	A24 family peptidase	-
HALHA_RS13065 (Halha_1788)	-	1819636..1820067 (-)	432	WP_015327440.1	type II secretion system protein	-
HALHA_RS08765 (Halha_1789)	-	1820090..1821331 (-)	1242	WP_342662856.1	type II secretion system F family protein	-
HALHA_RS08770 (Halha_1790)	pilT	1821313..1822356 (-)	1044	WP_015327442.1	type IV pilus twitching motility protein PilT	Machinery gene
HALHA_RS08775 (Halha_1791)	-	1822379..1824052 (-)	1674	WP_015327443.1	ATPase, T2SS/T4P/T4SS family	-

Sequence

Protein

• Length: 347 a.a.
• Molecular weight: 39245.95 Da
• Isoelectric Point: 6.5089

>NTDB_id=55578 HALHA_RS08770 WP_015327442.1 1821313..1822356(-) (pilT) [Halobacteroides halobius DSM 5150]
MKLENLLTEGIRLGASDIHLTMGTEPMVRVNGRLQKMNDKIVNKDKINNLVDELLEDRRNQFEADKELDFAYQWDGYRFR
VNAFYQRNNPALVLRIIPNQIDDLDSLGLPEKLKKLAVEPRGLFLVTGPTGSGKSTTLAAMINLVNQNYSKHIITLEDPI
EYIHQHKKSLVNQREVGRDTIDFASGLKAALRQDPDIILVGEMRDLETISTAITAAETGHLVLATLHTNNAAETIDRIIN
VFPPYQQEQIRTQLSMNLKGVLAQQLLPTVDGLERKVATELLLVNSAVKNLIREGKNHQLDSVMQTSKHEGMHTMDYSLR
DLYLQGKISHQVALEQANDRQTLEKLI

Nucleotide

• Length: 1044 bp

>NTDB_id=55578 HALHA_RS08770 WP_015327442.1 1821313..1822356(-) (pilT) [Halobacteroides halobius DSM 5150]
GTGAAATTAGAGAATTTATTAACTGAAGGGATTAGGTTAGGAGCTTCAGATATTCATTTGACTATGGGGACAGAACCTAT
GGTCAGAGTTAATGGTAGATTGCAAAAGATGAATGACAAAATTGTAAATAAAGATAAAATTAATAATTTAGTAGATGAAT
TATTAGAGGATAGAAGGAATCAATTTGAAGCAGATAAAGAATTAGACTTTGCTTATCAGTGGGATGGATATAGATTTAGA
GTTAATGCTTTTTATCAAAGAAATAATCCAGCTTTAGTATTACGAATTATTCCTAATCAGATTGATGATTTGGATAGCTT
AGGTTTGCCAGAAAAGTTAAAGAAATTAGCTGTAGAACCGAGAGGACTTTTTTTGGTTACTGGGCCAACTGGTAGTGGAA
AGTCAACTACTTTAGCTGCTATGATTAATTTAGTTAATCAAAATTATAGTAAGCATATTATTACTTTAGAGGATCCAATT
GAATATATACACCAACATAAAAAAAGTCTAGTTAATCAACGTGAAGTAGGCAGGGATACTATTGATTTTGCTAGTGGTCT
TAAGGCTGCTTTAAGGCAAGACCCTGATATCATTTTAGTTGGTGAAATGAGGGATTTAGAAACTATTTCAACTGCTATTA
CTGCTGCAGAGACTGGTCATTTAGTATTAGCAACCTTACATACTAATAATGCTGCTGAGACTATTGATCGTATTATTAAT
GTGTTTCCACCTTATCAACAGGAACAAATTAGAACACAGTTATCTATGAATTTAAAAGGAGTATTAGCCCAACAATTATT
ACCTACAGTTGATGGATTAGAACGAAAAGTAGCTACTGAACTATTATTAGTAAATTCTGCAGTTAAAAATTTAATTCGTG
AGGGAAAGAATCATCAATTAGATTCAGTAATGCAAACAAGTAAACATGAGGGTATGCATACTATGGATTATTCTTTACGA
GATTTATATTTACAAGGAAAAATTAGTCATCAAGTTGCATTAGAACAAGCAAATGATCGTCAGACATTAGAAAAGTTAAT
TTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (6-267)

3D structure

• AlphaFold DB: L0K9N2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	51.642	96.542	0.499
pilT	Neisseria meningitidis 8013	48.986	99.424	0.487
pilT	Neisseria gonorrhoeae MS11	48.696	99.424	0.484
pilT	Legionella pneumophila strain ERS1305867	48.673	97.695	0.476
pilT	Legionella pneumophila strain Lp02	48.673	97.695	0.476
pilT	Pseudomonas stutzeri DSM 10701	48.378	97.695	0.473
pilT	Acinetobacter nosocomialis M2	47.536	99.424	0.473
pilT	Acinetobacter baumannii D1279779	47.536	99.424	0.473
pilT	Acinetobacter baumannii strain A118	47.536	99.424	0.473
pilT	Acinetobacter baylyi ADP1	47.246	99.424	0.47
pilT	Pseudomonas aeruginosa PAK	47.788	97.695	0.467
pilU	Pseudomonas stutzeri DSM 10701	46.418	100	0.467
pilT	Vibrio cholerae strain A1552	48.048	95.965	0.461
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	48.048	95.965	0.461
pilU	Vibrio cholerae strain A1552	45.846	93.66	0.429
pilU	Acinetobacter baylyi ADP1	43.109	98.271	0.424
ctsE	Campylobacter jejuni subsp. jejuni 81-176	35.493	100	0.363
pilB/pilB1	Synechocystis sp. PCC 6803	38.65	93.948	0.363