Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   THEOS_RS09765 Genome accession   NC_019386
Coordinates   1866892..1867983 (+) Length   363 a.a.
NCBI ID   WP_016330153.1    Uniprot ID   K7R0Z1
Organism   Thermus oshimai JL-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1861892..1872983
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  THEOS_RS09740 (Theos_1966) ribH 1862044..1862520 (+) 477 WP_041436569.1 6,7-dimethyl-8-ribityllumazine synthase -
  THEOS_RS09745 (Theos_1967) - 1862504..1862908 (-) 405 WP_016330149.1 DUF4395 domain-containing protein -
  THEOS_RS09750 (Theos_1968) pgeF 1862979..1863707 (+) 729 WP_016330150.1 peptidoglycan editing factor PgeF -
  THEOS_RS09755 (Theos_1969) - 1863732..1864217 (+) 486 WP_016330151.1 YqeG family HAD IIIA-type phosphatase -
  THEOS_RS09760 (Theos_1970) pilF 1864214..1866880 (+) 2667 WP_016330152.1 type IV pilus assembly ATPase PilB Machinery gene
  THEOS_RS09765 (Theos_1971) pilT 1866892..1867983 (+) 1092 WP_016330153.1 type IV pilus twitching motility protein PilT Machinery gene
  THEOS_RS09770 (Theos_1972) gatB 1868001..1869431 (+) 1431 WP_016330154.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB -
  THEOS_RS09775 (Theos_1973) purM 1869436..1870437 (+) 1002 WP_016330155.1 phosphoribosylformylglycinamidine cyclo-ligase -
  THEOS_RS09780 (Theos_1974) - 1870434..1871063 (+) 630 WP_016330156.1 histidine phosphatase family protein -

Sequence

Protein


Download         Length: 363 a.a.        Molecular weight: 40238.55 Da        Isoelectric Point: 7.2792

>NTDB_id=54368 THEOS_RS09765 WP_016330153.1 1866892..1867983(+) (pilT) [Thermus oshimai JL-2]
MPKAPDIVDLLSLAVERGASDLVITVGLPPMIKVDGEFHPTEYEPLTPQETRRLMYALMDEKQQRVFEEEKELDFSFSLP
GKGRYRVNVFLQRGSVGGVLRVVPAHIKSFEELGLPKNIAEIALSPRGLVLVTGPTGSGKSTTLASMIDYINERKPVHIV
TIEDPIEFFHRHKRAIINQREIGSDTHGFHKALRSVLRQAPDVILVGEMRDYETIAAAITAAETGHLVMGTLHTNSAPET
IDRIIDVFPENQQEQVRVQLSNNLVAVLTQQLLPKAFGGGRVLAYELMIATPAVRALIREGKSHQLRSVIQTGGQYGMVT
MDACLADLYKRKLITYEMGLARAVDPKEFMRLAGAGEKEGRPK

Nucleotide


Download         Length: 1092 bp        

>NTDB_id=54368 THEOS_RS09765 WP_016330153.1 1866892..1867983(+) (pilT) [Thermus oshimai JL-2]
ATGCCGAAAGCGCCGGACATCGTAGATCTGCTTTCCCTGGCCGTGGAGCGGGGCGCCAGCGACCTGGTCATCACCGTGGG
CCTTCCGCCCATGATCAAGGTGGACGGGGAGTTCCACCCCACGGAGTACGAGCCCCTCACCCCCCAGGAGACCCGCCGCC
TCATGTACGCCCTCATGGACGAGAAGCAGCAGCGGGTCTTTGAGGAGGAGAAAGAGCTGGACTTCTCCTTCAGCCTCCCC
GGCAAGGGGCGCTACCGGGTGAACGTCTTCCTCCAGCGGGGGAGCGTGGGCGGGGTCTTGCGGGTGGTGCCGGCCCATAT
CAAGAGCTTTGAGGAGCTGGGCCTGCCCAAGAACATCGCCGAGATCGCCCTGAGCCCCCGGGGCCTGGTCCTGGTCACGG
GGCCTACGGGAAGCGGGAAGAGCACCACCCTGGCCTCCATGATCGACTACATCAACGAACGCAAGCCCGTGCACATCGTG
ACCATCGAGGACCCCATCGAGTTCTTCCACCGCCACAAGAGGGCCATCATCAACCAGCGGGAGATCGGTTCGGACACCCA
CGGCTTCCACAAGGCCCTAAGGAGCGTCCTCCGCCAGGCCCCGGACGTGATCCTGGTGGGGGAGATGCGGGACTACGAGA
CCATCGCCGCGGCCATCACCGCCGCGGAGACCGGCCACCTGGTCATGGGCACCCTGCACACCAACTCCGCCCCCGAGACC
ATCGACCGCATCATCGACGTCTTCCCCGAGAACCAACAGGAGCAGGTGCGGGTGCAGCTTTCCAACAACCTGGTGGCCGT
CCTCACCCAGCAGCTCCTGCCCAAGGCCTTCGGCGGGGGCAGGGTCCTGGCCTACGAGCTCATGATCGCCACCCCCGCGG
TGCGGGCCCTCATCCGCGAGGGGAAGAGCCACCAGCTAAGGAGCGTGATCCAGACCGGGGGGCAGTACGGGATGGTCACC
ATGGACGCCTGCCTGGCGGACCTCTACAAGCGCAAGCTCATCACCTACGAGATGGGCCTGGCCCGGGCCGTGGACCCCAA
GGAGTTCATGCGCCTGGCGGGAGCAGGGGAAAAGGAGGGCCGGCCCAAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K7R0Z1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

67.422

97.245

0.656
  pilT Acinetobacter baylyi ADP1

54.651

94.766

0.518
  pilT Acinetobacter nosocomialis M2

53.913

95.041

0.512
  pilT Acinetobacter baumannii strain A118

53.913

95.041

0.512
  pilT Acinetobacter baumannii D1279779

53.913

95.041

0.512
  pilT Pseudomonas aeruginosa PAK

53.779

94.766

0.51
  pilT Pseudomonas stutzeri DSM 10701

53.488

94.766

0.507
  pilT Neisseria meningitidis 8013

52.071

93.113

0.485
  pilT Legionella pneumophila strain ERS1305867

50.872

94.766

0.482
  pilT Legionella pneumophila strain Lp02

50.872

94.766

0.482
  pilT Vibrio cholerae strain A1552

52.584

90.634

0.477
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.584

90.634

0.477
  pilT Neisseria gonorrhoeae MS11

51.335

92.837

0.477
  pilU Pseudomonas stutzeri DSM 10701

47.23

94.49

0.446
  pilU Acinetobacter baylyi ADP1

41.62

98.623

0.41
  pilU Vibrio cholerae strain A1552

44.012

92.011

0.405

Multiple sequence alignment