Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   JVX91_RS10305 Genome accession   NZ_CP070356
Coordinates   2212522..2212947 (+) Length   141 a.a.
NCBI ID   WP_205339978.1    Uniprot ID   -
Organism   Pseudomonas sp. PDNC002     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2207522..2217947
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JVX91_RS10280 (JVX91_10280) yacG 2207622..2207825 (-) 204 WP_205339126.1 DNA gyrase inhibitor YacG -
  JVX91_RS10285 (JVX91_10285) coaE 2207822..2208430 (-) 609 WP_205339127.1 dephospho-CoA kinase -
  JVX91_RS10290 (JVX91_10290) pilD 2208496..2209368 (-) 873 WP_205339128.1 A24 family peptidase Machinery gene
  JVX91_RS10295 (JVX91_10295) pilC 2209372..2210589 (-) 1218 WP_205339129.1 type II secretion system F family protein Machinery gene
  JVX91_RS10300 (JVX91_10300) pilB 2210593..2212296 (-) 1704 WP_205339130.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JVX91_RS10305 (JVX91_10305) pilA 2212522..2212947 (+) 426 WP_205339978.1 pilin Machinery gene
  JVX91_RS10310 (JVX91_10310) - 2213154..2214914 (+) 1761 WP_205339131.1 hypothetical protein -
  JVX91_RS10315 (JVX91_10315) rffA 2214941..2216089 (+) 1149 WP_205339132.1 dTDP-4-amino-4,6-dideoxygalactose transaminase -
  JVX91_RS10320 (JVX91_10320) - 2216114..2217589 (+) 1476 WP_205339133.1 hypothetical protein -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 14087.15 Da        Isoelectric Point: 9.0590

>NTDB_id=539368 JVX91_RS10305 WP_205339978.1 2212522..2212947(+) (pilA) [Pseudomonas sp. PDNC002]
MKAQKGFTLIELMIVVAIIGILAAIALPAYQDYTVRARVSEGLVLASAAKTAVAENAANGTSPLSAGWTAPTATKNVSSV
SVNGGTGEISVVYQAAAGSVNLKLTPSSGGSALSAGAVPTGAITWKCTTSTDAKYVPAECR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=539368 JVX91_RS10305 WP_205339978.1 2212522..2212947(+) (pilA) [Pseudomonas sp. PDNC002]
ATGAAAGCTCAAAAAGGCTTTACTCTGATCGAATTGATGATCGTTGTTGCGATCATCGGCATTCTGGCCGCCATCGCTCT
GCCGGCCTATCAGGACTACACCGTGCGTGCGCGTGTGAGTGAAGGTCTGGTTCTGGCTTCTGCTGCTAAGACCGCTGTAG
CAGAAAACGCTGCAAACGGTACTTCTCCGCTGAGCGCTGGCTGGACTGCTCCAACTGCCACCAAGAACGTATCCAGCGTT
TCGGTTAACGGCGGCACTGGCGAAATCTCCGTCGTGTACCAAGCTGCCGCTGGTAGCGTGAACCTCAAACTGACTCCGTC
TTCGGGTGGCTCCGCACTGAGCGCCGGTGCCGTTCCGACCGGTGCTATCACCTGGAAGTGCACCACTTCTACCGATGCGA
AGTATGTTCCTGCTGAATGCCGCTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-141)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

54.717

100

0.617
  comP Acinetobacter baylyi ADP1

51.592

100

0.574
  pilA2 Legionella pneumophila strain ERS1305867

55.245

100

0.56
  pilA2 Legionella pneumophila str. Paris

55.245

100

0.56
  pilA/pilA1 Eikenella corrodens VA1

41.615

100

0.475
  pilE Neisseria gonorrhoeae strain FA1090

40.741

100

0.468
  pilE Neisseria gonorrhoeae MS11

40.491

100

0.468
  pilA Pseudomonas aeruginosa PAK

40

100

0.44
  pilA Acinetobacter baumannii strain A118

41.844

100

0.418
  pilA/pilAI Pseudomonas stutzeri DSM 10701

38.514

100

0.404
  pilA Vibrio cholerae strain A1552

36.667

100

0.39
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

36.667

100

0.39
  pilA Vibrio cholerae C6706

36.667

100

0.39
  pilA Haemophilus influenzae Rd KW20

39.855

97.872

0.39
  pilA/pilAII Pseudomonas stutzeri DSM 10701

37.589

100

0.376