Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   JVX91_RS10300 Genome accession   NZ_CP070356
Coordinates   2210593..2212296 (-) Length   567 a.a.
NCBI ID   WP_205339130.1    Uniprot ID   -
Organism   Pseudomonas sp. PDNC002     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2205593..2217296
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JVX91_RS10265 (JVX91_10265) - 2206104..2206538 (+) 435 WP_205339977.1 FAD/FMN-containing dehydrogenase -
  JVX91_RS10270 (JVX91_10270) - 2206535..2207224 (+) 690 WP_205339124.1 energy-coupling factor ABC transporter permease -
  JVX91_RS10275 (JVX91_10275) - 2207332..2207547 (+) 216 WP_205339125.1 hypothetical protein -
  JVX91_RS10280 (JVX91_10280) yacG 2207622..2207825 (-) 204 WP_205339126.1 DNA gyrase inhibitor YacG -
  JVX91_RS10285 (JVX91_10285) coaE 2207822..2208430 (-) 609 WP_205339127.1 dephospho-CoA kinase -
  JVX91_RS10290 (JVX91_10290) pilD 2208496..2209368 (-) 873 WP_205339128.1 A24 family peptidase Machinery gene
  JVX91_RS10295 (JVX91_10295) pilC 2209372..2210589 (-) 1218 WP_205339129.1 type II secretion system F family protein Machinery gene
  JVX91_RS10300 (JVX91_10300) pilB 2210593..2212296 (-) 1704 WP_205339130.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JVX91_RS10305 (JVX91_10305) pilA 2212522..2212947 (+) 426 WP_205339978.1 pilin Machinery gene
  JVX91_RS10310 (JVX91_10310) - 2213154..2214914 (+) 1761 WP_205339131.1 hypothetical protein -
  JVX91_RS10315 (JVX91_10315) rffA 2214941..2216089 (+) 1149 WP_205339132.1 dTDP-4-amino-4,6-dideoxygalactose transaminase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62538.58 Da        Isoelectric Point: 5.7286

>NTDB_id=539367 JVX91_RS10300 WP_205339130.1 2210593..2212296(-) (pilB) [Pseudomonas sp. PDNC002]
MNDQVVLSGLARQLVHSELLDEKSAQQAQLQAQRNKLSLVTYLVQNKLVKSRPLTELAAEQFGVAFCDLSSLDRESLPKE
LVSEKLIRQHRVLPLWRRGNKLFVGLSDPTNHQAITDVQFSTGLTTEAILVEDDKLGEAIEKLFESVTGGLDDLGDVDLD
GLDIESGNTEHKDEPIGGDADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKVYRVRFRTDGMLHETAKPPIQLASRISAR
LKVMAGLDISERRKPQDGRIKMRVSKSKSIDFRVNTLPTLWGEKIVMRILDSASAQMGIDALGYEDAQKQLYMDALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTPDINISTAEDPVEINLEGINQVNVNPRQGMDFAQALRAFLRQDPDVIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVASFNLATSTNLIIAQRLARKLCPTCKKEHDVPRDALLHEGFP
EDKIGSFKLYSPVGCENCKGGYKGRVGIYEVVKITPALQRIIMEEGNSIQIAEQARKEGFNDLRASGLVKAMQGITSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=539367 JVX91_RS10300 WP_205339130.1 2210593..2212296(-) (pilB) [Pseudomonas sp. PDNC002]
ATGAACGATCAAGTCGTGCTGTCAGGCCTCGCCCGCCAGCTGGTGCACTCCGAGCTTCTCGATGAGAAAAGCGCGCAGCA
AGCTCAGCTCCAGGCACAGCGAAATAAGCTGTCGCTGGTGACTTACCTCGTACAAAACAAGCTGGTCAAAAGCAGACCAC
TGACCGAACTGGCCGCAGAGCAGTTCGGCGTGGCTTTCTGCGACCTCAGCAGCCTTGATCGAGAGAGCCTCCCGAAGGAA
CTGGTGAGTGAGAAGCTAATCCGCCAACACCGGGTGCTCCCGCTGTGGCGTCGCGGCAACAAGCTATTTGTCGGCCTCTC
GGACCCGACCAACCACCAAGCCATCACGGACGTCCAGTTCAGTACAGGCCTCACCACGGAAGCCATCCTGGTGGAGGATG
ACAAGCTCGGTGAGGCTATCGAAAAGCTATTCGAAAGCGTCACTGGCGGGCTGGACGATCTGGGCGATGTCGATCTCGAC
GGACTAGACATCGAGAGTGGCAACACCGAGCACAAGGATGAGCCCATTGGGGGCGACGCAGATGACGCGCCAGTGGTGCG
CTTCGTCAACAAGATGCTTCTGGATGCCATCAAGGGCGGCTCTTCCGACCTGCACTTCGAGCCCTACGAGAAAGTCTACC
GCGTGCGCTTCCGCACCGACGGCATGCTGCACGAGACGGCCAAGCCCCCAATCCAATTGGCCAGTCGTATCTCCGCGCGC
CTGAAGGTAATGGCCGGCCTGGATATCTCTGAGCGACGCAAGCCTCAGGACGGCCGCATTAAGATGCGAGTATCGAAGAG
CAAATCCATCGACTTCCGCGTCAACACCCTGCCAACCCTGTGGGGCGAGAAGATCGTCATGCGGATTCTCGACTCCGCGA
GCGCCCAGATGGGTATCGACGCCCTGGGCTACGAGGATGCTCAGAAGCAGCTCTACATGGATGCGCTCAAGCAACCGCAA
GGCATGATTCTGGTGACAGGCCCGACCGGATCGGGAAAGACCGTCTCGCTCTACACCGGCCTGAATATCCTCAACACCCC
GGACATCAATATTTCCACTGCAGAAGACCCGGTGGAGATCAACCTCGAAGGTATCAACCAGGTCAACGTCAACCCCCGCC
AAGGGATGGACTTCGCCCAAGCGCTCCGGGCATTCCTGCGCCAAGATCCCGACGTGATCATGGTCGGCGAGATCCGTGAC
CTGGAAACGGCGGAAATCGCCATCAAAGCCGCTCAAACCGGCCACATGGTAATGTCTACACTGCACACCAACAGTGCCGC
CGAGACACTGACGCGCTTGCTTAACATGGGTGTCGCATCCTTCAACCTGGCGACCTCCACCAACCTGATCATTGCCCAGC
GACTCGCTCGTAAACTCTGCCCGACCTGCAAAAAAGAACACGACGTGCCTCGAGATGCTCTGCTGCATGAAGGCTTCCCG
GAGGACAAGATCGGGTCCTTCAAACTGTACTCCCCCGTCGGGTGTGAAAATTGCAAGGGCGGTTATAAAGGTCGAGTCGG
TATTTATGAAGTGGTTAAAATCACGCCAGCCTTGCAGCGGATTATCATGGAGGAAGGTAACTCCATTCAGATCGCCGAAC
AGGCCCGGAAAGAAGGCTTCAACGATCTGCGCGCCTCAGGGCTGGTGAAAGCCATGCAAGGCATCACCAGCCTCGAGGAA
GTCAACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.615

99.295

0.582
  pilB Acinetobacter baumannii D1279779

58.156

99.471

0.578
  pilB Legionella pneumophila strain ERS1305867

54.145

100

0.541
  pilB Vibrio cholerae strain A1552

51.159

98.942

0.506
  pilB Vibrio parahaemolyticus RIMD 2210633

52.865

95.414

0.504
  pilF Neisseria gonorrhoeae MS11

49.911

99.295

0.496
  pilB Vibrio campbellii strain DS40M4

51.763

95.062

0.492
  pilF Thermus thermophilus HB27

38.393

98.765

0.379
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.962

93.122

0.372