Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   JH304_RS05260 Genome accession   NZ_CP066933
Coordinates   1239188..1239628 (+) Length   146 a.a.
NCBI ID   WP_011038214.1    Uniprot ID   Q8P671
Organism   Xanthomonas campestris pv. campestris strain 10108     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1192515..1248695 1239188..1239628 within 0


Gene organization within MGE regions


Location: 1192515..1248695
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JH304_RS05005 (JH304_04995) - 1194119..1194547 (+) 429 WP_145998822.1 hypothetical protein -
  JH304_RS05010 (JH304_05000) - 1194932..1195720 (-) 789 WP_145998823.1 hypothetical protein -
  JH304_RS05015 (JH304_05005) - 1195794..1196687 (-) 894 WP_145998824.1 hypothetical protein -
  JH304_RS05020 (JH304_05010) - 1196853..1197233 (-) 381 WP_172973787.1 hypothetical protein -
  JH304_RS05025 (JH304_05015) - 1197230..1197544 (-) 315 WP_218065344.1 hypothetical protein -
  JH304_RS05030 (JH304_05020) - 1197594..1198355 (-) 762 WP_100666316.1 hypothetical protein -
  JH304_RS05035 (JH304_05025) - 1198533..1198832 (+) 300 WP_230428394.1 helix-turn-helix transcriptional regulator -
  JH304_RS05040 (JH304_05030) - 1198901..1199332 (+) 432 WP_100666318.1 hypothetical protein -
  JH304_RS05045 (JH304_05035) - 1199337..1199789 (-) 453 WP_145998825.1 hypothetical protein -
  JH304_RS05050 (JH304_05040) - 1199992..1201392 (-) 1401 WP_218065345.1 site-specific integrase -
  JH304_RS05055 (JH304_05045) - 1202000..1202515 (-) 516 WP_100666321.1 hypothetical protein -
  JH304_RS05060 (JH304_05050) - 1202604..1203089 (+) 486 WP_145998826.1 hypothetical protein -
  JH304_RS05065 (JH304_05055) - 1203222..1204706 (-) 1485 WP_100666322.1 hypothetical protein -
  JH304_RS05070 (JH304_05060) - 1204786..1205751 (-) 966 WP_145998827.1 hypothetical protein -
  JH304_RS05075 (JH304_05065) - 1206141..1206401 (+) 261 WP_145998828.1 hypothetical protein -
  JH304_RS05080 (JH304_05070) - 1206388..1206885 (-) 498 WP_152091502.1 hypothetical protein -
  JH304_RS05085 (JH304_05075) - 1206995..1207525 (-) 531 WP_152091503.1 hypothetical protein -
  JH304_RS05090 (JH304_05080) - 1207560..1208372 (-) 813 WP_152091504.1 hypothetical protein -
  JH304_RS05095 (JH304_05085) - 1208369..1208677 (-) 309 WP_152091505.1 hypothetical protein -
  JH304_RS05100 (JH304_05090) radC 1208888..1209367 (+) 480 WP_152091506.1 DNA repair protein RadC -
  JH304_RS05105 (JH304_05095) - 1209436..1209612 (+) 177 WP_172973789.1 hypothetical protein -
  JH304_RS05110 (JH304_05100) - 1209832..1210473 (+) 642 WP_152091507.1 DUF1629 domain-containing protein -
  JH304_RS05115 (JH304_05105) - 1210509..1213094 (+) 2586 WP_218065346.1 XVIPCD domain-containing protein -
  JH304_RS05120 (JH304_05110) - 1213334..1214137 (-) 804 WP_230428395.1 hypothetical protein -
  JH304_RS05125 (JH304_05115) - 1214421..1214927 (-) 507 WP_230428396.1 hypothetical protein -
  JH304_RS05130 (JH304_05120) - 1215004..1215639 (+) 636 WP_230428397.1 hypothetical protein -
  JH304_RS05135 (JH304_05125) - 1215757..1215915 (+) 159 WP_172973790.1 hypothetical protein -
  JH304_RS05145 (JH304_05135) - 1217984..1218460 (-) 477 WP_152091510.1 hypothetical protein -
  JH304_RS05150 (JH304_05140) - 1218622..1219380 (-) 759 WP_234565086.1 ATP-dependent endonuclease -
  JH304_RS05155 (JH304_05145) - 1219990..1219991 (-) 2 WP_087942062.1 IS5 family transposase -
  JH304_RS05160 (JH304_05150) - 1220236..1221447 (-) 1212 WP_218065348.1 AAA family ATPase -
  JH304_RS05165 (JH304_05155) traD 1221983..1222282 (-) 300 WP_100666201.1 conjugal transfer protein TraD -
  JH304_RS05170 (JH304_05160) - 1222549..1224144 (+) 1596 WP_100666199.1 MobA/MobL family protein -
  JH304_RS05175 (JH304_05165) - 1224197..1224538 (-) 342 WP_100666197.1 hypothetical protein -
  JH304_RS05180 (JH304_05170) - 1225232..1225660 (+) 429 WP_012437589.1 hypothetical protein -
  JH304_RS05185 (JH304_05175) - 1225903..1226133 (-) 231 WP_157382680.1 hypothetical protein -
  JH304_RS05190 (JH304_05180) - 1226197..1226343 (+) 147 WP_040940792.1 hypothetical protein -
  JH304_RS05195 (JH304_05185) - 1226528..1226923 (+) 396 WP_016945003.1 hypothetical protein -
  JH304_RS05200 (JH304_05190) - 1227014..1227406 (+) 393 WP_012437593.1 H-NS family nucleoid-associated regulatory protein -
  JH304_RS05205 (JH304_05195) glgX 1227940..1230069 (-) 2130 WP_016945001.1 glycogen debranching protein GlgX -
  JH304_RS05210 (JH304_05200) rimK 1230563..1231438 (+) 876 WP_016945000.1 30S ribosomal protein S6--L-glutamate ligase -
  JH304_RS05215 (JH304_05205) - 1231661..1232134 (+) 474 WP_012437597.1 hypothetical protein -
  JH304_RS05220 (JH304_05210) - 1232165..1232842 (+) 678 WP_003490678.1 response regulator transcription factor -
  JH304_RS05225 (JH304_05215) - 1232835..1234169 (+) 1335 WP_012437598.1 HAMP domain-containing sensor histidine kinase -
  JH304_RS05230 (JH304_05220) - 1234312..1234803 (-) 492 WP_011038220.1 GNAT family N-acetyltransferase -
  JH304_RS05235 (JH304_05225) - 1234800..1235090 (-) 291 WP_011038219.1 DUF1778 domain-containing protein -
  JH304_RS05240 (JH304_05230) - 1235165..1235566 (-) 402 WP_019237348.1 SymE family type I addiction module toxin -
  JH304_RS05245 (JH304_05235) coaE 1236098..1236721 (-) 624 WP_011038217.1 dephospho-CoA kinase -
  JH304_RS05250 (JH304_05240) - 1236735..1237598 (-) 864 WP_011038216.1 A24 family peptidase -
  JH304_RS05255 (JH304_05245) pilC 1237605..1238861 (-) 1257 WP_011038215.1 type II secretion system F family protein Machinery gene
  JH304_RS05260 (JH304_05250) pilE 1239188..1239628 (+) 441 WP_011038214.1 pilin Machinery gene
  JH304_RS05265 (JH304_05255) - 1239708..1240142 (+) 435 WP_011038213.1 pilin -
  JH304_RS05270 (JH304_05260) pilB 1240207..1241940 (+) 1734 WP_011038212.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JH304_RS05280 (JH304_05270) pilR 1242359..1243753 (-) 1395 WP_012437607.1 sigma-54 dependent transcriptional regulator Regulator
  JH304_RS05285 (JH304_05275) - 1243962..1245572 (-) 1611 WP_012437608.1 HAMP domain-containing sensor histidine kinase -
  JH304_RS05290 (JH304_05280) sucC 1245806..1246975 (+) 1170 WP_012437609.1 ADP-forming succinate--CoA ligase subunit beta -
  JH304_RS05295 (JH304_05285) sucD 1247000..1247875 (+) 876 WP_011038208.1 succinate--CoA ligase subunit alpha -
  JH304_RS05300 (JH304_05290) - 1247979..1248389 (+) 411 WP_029628876.1 DNA-binding protein -
  JH304_RS05305 (JH304_05295) - 1248393..1248695 (+) 303 WP_011038207.1 type II toxin-antitoxin system RelE/ParE family toxin -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 14797.93 Da        Isoelectric Point: 9.0090

>NTDB_id=523354 JH304_RS05260 WP_011038214.1 1239188..1239628(+) (pilE) [Xanthomonas campestris pv. campestris strain 10108]
MKKQNGFTLIELMIVVAIIAILAAIALPAYQDYLARSQVSEGLSLASGAKTAVAETYANTGAFPATNAAAGLEAAANIKG
KYVTSVTVGAGGIITAAFNTANAKLSGKNLVLTPTDNNGSISWGCTNGTTIDQKYLPTSCRTAAAP

Nucleotide


Download         Length: 441 bp        

>NTDB_id=523354 JH304_RS05260 WP_011038214.1 1239188..1239628(+) (pilE) [Xanthomonas campestris pv. campestris strain 10108]
ATGAAAAAGCAAAATGGTTTTACACTGATCGAACTCATGATCGTCGTTGCGATCATCGCTATTCTGGCCGCTATCGCTTT
GCCGGCGTACCAGGATTACCTCGCTCGTTCGCAGGTTTCGGAAGGCTTGTCTTTGGCGTCGGGTGCAAAGACAGCTGTCG
CTGAAACTTATGCTAACACCGGTGCCTTCCCGGCGACCAATGCCGCCGCTGGCCTTGAGGCTGCTGCGAACATCAAGGGT
AAGTACGTTACGTCTGTGACGGTGGGGGCGGGTGGGATCATCACCGCGGCATTCAATACTGCTAATGCAAAGTTGAGTGG
CAAGAATCTGGTCCTGACCCCGACTGACAATAACGGTTCGATCAGCTGGGGGTGCACCAACGGCACCACGATTGATCAGA
AGTATCTGCCGACCTCTTGCCGCACTGCGGCGGCTCCGTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8P671

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae MS11

46.875

100

0.514
  pilE Neisseria gonorrhoeae strain FA1090

46.541

100

0.507
  pilA2 Legionella pneumophila str. Paris

52.857

95.89

0.507
  pilA2 Legionella pneumophila strain ERS1305867

52.143

95.89

0.5
  pilA/pilA1 Eikenella corrodens VA1

46.452

100

0.493
  comP Acinetobacter baylyi ADP1

48

100

0.493
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

37.5

100

0.473
  pilA Ralstonia pseudosolanacearum GMI1000

40.244

100

0.452
  pilA Acinetobacter baumannii strain A118

41.333

100

0.425
  pilA Pseudomonas aeruginosa PAK

40.789

100

0.425
  pilA Vibrio campbellii strain DS40M4

40.268

100

0.411
  pilA Vibrio cholerae C6706

38.411

100

0.397
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

38.411

100

0.397
  pilA Vibrio cholerae strain A1552

38.411

100

0.397
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40.288

95.205

0.384
  pilA Vibrio parahaemolyticus RIMD 2210633

42.4

85.616

0.363