Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   JH304_RS05270 Genome accession   NZ_CP066933
Coordinates   1240207..1241940 (+) Length   577 a.a.
NCBI ID   WP_011038212.1    Uniprot ID   Q8P673
Organism   Xanthomonas campestris pv. campestris strain 10108     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1192515..1248695 1240207..1241940 within 0


Gene organization within MGE regions


Location: 1192515..1248695
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JH304_RS05005 (JH304_04995) - 1194119..1194547 (+) 429 WP_145998822.1 hypothetical protein -
  JH304_RS05010 (JH304_05000) - 1194932..1195720 (-) 789 WP_145998823.1 hypothetical protein -
  JH304_RS05015 (JH304_05005) - 1195794..1196687 (-) 894 WP_145998824.1 hypothetical protein -
  JH304_RS05020 (JH304_05010) - 1196853..1197233 (-) 381 WP_172973787.1 hypothetical protein -
  JH304_RS05025 (JH304_05015) - 1197230..1197544 (-) 315 WP_218065344.1 hypothetical protein -
  JH304_RS05030 (JH304_05020) - 1197594..1198355 (-) 762 WP_100666316.1 hypothetical protein -
  JH304_RS05035 (JH304_05025) - 1198533..1198832 (+) 300 WP_230428394.1 helix-turn-helix transcriptional regulator -
  JH304_RS05040 (JH304_05030) - 1198901..1199332 (+) 432 WP_100666318.1 hypothetical protein -
  JH304_RS05045 (JH304_05035) - 1199337..1199789 (-) 453 WP_145998825.1 hypothetical protein -
  JH304_RS05050 (JH304_05040) - 1199992..1201392 (-) 1401 WP_218065345.1 site-specific integrase -
  JH304_RS05055 (JH304_05045) - 1202000..1202515 (-) 516 WP_100666321.1 hypothetical protein -
  JH304_RS05060 (JH304_05050) - 1202604..1203089 (+) 486 WP_145998826.1 hypothetical protein -
  JH304_RS05065 (JH304_05055) - 1203222..1204706 (-) 1485 WP_100666322.1 hypothetical protein -
  JH304_RS05070 (JH304_05060) - 1204786..1205751 (-) 966 WP_145998827.1 hypothetical protein -
  JH304_RS05075 (JH304_05065) - 1206141..1206401 (+) 261 WP_145998828.1 hypothetical protein -
  JH304_RS05080 (JH304_05070) - 1206388..1206885 (-) 498 WP_152091502.1 hypothetical protein -
  JH304_RS05085 (JH304_05075) - 1206995..1207525 (-) 531 WP_152091503.1 hypothetical protein -
  JH304_RS05090 (JH304_05080) - 1207560..1208372 (-) 813 WP_152091504.1 hypothetical protein -
  JH304_RS05095 (JH304_05085) - 1208369..1208677 (-) 309 WP_152091505.1 hypothetical protein -
  JH304_RS05100 (JH304_05090) radC 1208888..1209367 (+) 480 WP_152091506.1 DNA repair protein RadC -
  JH304_RS05105 (JH304_05095) - 1209436..1209612 (+) 177 WP_172973789.1 hypothetical protein -
  JH304_RS05110 (JH304_05100) - 1209832..1210473 (+) 642 WP_152091507.1 DUF1629 domain-containing protein -
  JH304_RS05115 (JH304_05105) - 1210509..1213094 (+) 2586 WP_218065346.1 XVIPCD domain-containing protein -
  JH304_RS05120 (JH304_05110) - 1213334..1214137 (-) 804 WP_230428395.1 hypothetical protein -
  JH304_RS05125 (JH304_05115) - 1214421..1214927 (-) 507 WP_230428396.1 hypothetical protein -
  JH304_RS05130 (JH304_05120) - 1215004..1215639 (+) 636 WP_230428397.1 hypothetical protein -
  JH304_RS05135 (JH304_05125) - 1215757..1215915 (+) 159 WP_172973790.1 hypothetical protein -
  JH304_RS05145 (JH304_05135) - 1217984..1218460 (-) 477 WP_152091510.1 hypothetical protein -
  JH304_RS05150 (JH304_05140) - 1218622..1219380 (-) 759 WP_234565086.1 ATP-dependent endonuclease -
  JH304_RS05155 (JH304_05145) - 1219990..1219991 (-) 2 WP_087942062.1 IS5 family transposase -
  JH304_RS05160 (JH304_05150) - 1220236..1221447 (-) 1212 WP_218065348.1 AAA family ATPase -
  JH304_RS05165 (JH304_05155) traD 1221983..1222282 (-) 300 WP_100666201.1 conjugal transfer protein TraD -
  JH304_RS05170 (JH304_05160) - 1222549..1224144 (+) 1596 WP_100666199.1 MobA/MobL family protein -
  JH304_RS05175 (JH304_05165) - 1224197..1224538 (-) 342 WP_100666197.1 hypothetical protein -
  JH304_RS05180 (JH304_05170) - 1225232..1225660 (+) 429 WP_012437589.1 hypothetical protein -
  JH304_RS05185 (JH304_05175) - 1225903..1226133 (-) 231 WP_157382680.1 hypothetical protein -
  JH304_RS05190 (JH304_05180) - 1226197..1226343 (+) 147 WP_040940792.1 hypothetical protein -
  JH304_RS05195 (JH304_05185) - 1226528..1226923 (+) 396 WP_016945003.1 hypothetical protein -
  JH304_RS05200 (JH304_05190) - 1227014..1227406 (+) 393 WP_012437593.1 H-NS family nucleoid-associated regulatory protein -
  JH304_RS05205 (JH304_05195) glgX 1227940..1230069 (-) 2130 WP_016945001.1 glycogen debranching protein GlgX -
  JH304_RS05210 (JH304_05200) rimK 1230563..1231438 (+) 876 WP_016945000.1 30S ribosomal protein S6--L-glutamate ligase -
  JH304_RS05215 (JH304_05205) - 1231661..1232134 (+) 474 WP_012437597.1 hypothetical protein -
  JH304_RS05220 (JH304_05210) - 1232165..1232842 (+) 678 WP_003490678.1 response regulator transcription factor -
  JH304_RS05225 (JH304_05215) - 1232835..1234169 (+) 1335 WP_012437598.1 HAMP domain-containing sensor histidine kinase -
  JH304_RS05230 (JH304_05220) - 1234312..1234803 (-) 492 WP_011038220.1 GNAT family N-acetyltransferase -
  JH304_RS05235 (JH304_05225) - 1234800..1235090 (-) 291 WP_011038219.1 DUF1778 domain-containing protein -
  JH304_RS05240 (JH304_05230) - 1235165..1235566 (-) 402 WP_019237348.1 SymE family type I addiction module toxin -
  JH304_RS05245 (JH304_05235) coaE 1236098..1236721 (-) 624 WP_011038217.1 dephospho-CoA kinase -
  JH304_RS05250 (JH304_05240) - 1236735..1237598 (-) 864 WP_011038216.1 A24 family peptidase -
  JH304_RS05255 (JH304_05245) pilC 1237605..1238861 (-) 1257 WP_011038215.1 type II secretion system F family protein Machinery gene
  JH304_RS05260 (JH304_05250) pilE 1239188..1239628 (+) 441 WP_011038214.1 pilin Machinery gene
  JH304_RS05265 (JH304_05255) - 1239708..1240142 (+) 435 WP_011038213.1 pilin -
  JH304_RS05270 (JH304_05260) pilB 1240207..1241940 (+) 1734 WP_011038212.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JH304_RS05280 (JH304_05270) pilR 1242359..1243753 (-) 1395 WP_012437607.1 sigma-54 dependent transcriptional regulator Regulator
  JH304_RS05285 (JH304_05275) - 1243962..1245572 (-) 1611 WP_012437608.1 HAMP domain-containing sensor histidine kinase -
  JH304_RS05290 (JH304_05280) sucC 1245806..1246975 (+) 1170 WP_012437609.1 ADP-forming succinate--CoA ligase subunit beta -
  JH304_RS05295 (JH304_05285) sucD 1247000..1247875 (+) 876 WP_011038208.1 succinate--CoA ligase subunit alpha -
  JH304_RS05300 (JH304_05290) - 1247979..1248389 (+) 411 WP_029628876.1 DNA-binding protein -
  JH304_RS05305 (JH304_05295) - 1248393..1248695 (+) 303 WP_011038207.1 type II toxin-antitoxin system RelE/ParE family toxin -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62785.06 Da        Isoelectric Point: 5.5903

>NTDB_id=523355 JH304_RS05270 WP_011038212.1 1240207..1241940(+) (pilB) [Xanthomonas campestris pv. campestris strain 10108]
MSVVLTANLVGITGIARRLVQDGALEEAVARGAMDQAALAKVPLPQWFAEKKLVSAAQLASANAVEFGMPLMDVSVFDAS
QNAVKLVSEELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNAALGSSLG
DDDDEMGDLDVSTGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDASAAKLGIEKLGYEADQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
EHALLAEGFTPEQVANIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQKIGIRDLRQSALMK
ASHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=523355 JH304_RS05270 WP_011038212.1 1240207..1241940(+) (pilB) [Xanthomonas campestris pv. campestris strain 10108]
ATGAGCGTTGTGCTAACGGCTAATCTTGTTGGAATCACGGGCATTGCTCGACGTCTTGTGCAAGACGGTGCGCTCGAAGA
GGCGGTTGCTCGTGGTGCGATGGATCAGGCAGCACTCGCCAAAGTGCCTTTACCCCAGTGGTTTGCTGAAAAAAAGCTAG
TCTCTGCTGCGCAGCTCGCCTCTGCCAATGCAGTTGAGTTCGGTATGCCGCTGATGGATGTGTCGGTGTTCGACGCCAGC
CAGAACGCGGTCAAGCTGGTCAGTGAGGAGTTGCTTCAGAAGCATCAGGTACTGCCGCTGTTCAAGCGCGGCAACCGGTT
GTTCGTGGGGGTGAGCAATCCGACGCAGACCAGGGCGCTGGACGATATCAAGTTCCACACCAATCTGGTGGTCGAGCCGA
TCCTGGTAGACGAAGACCAGATCCGTCGCACATTGGAGCAGTGGCAGGCCAGCAATGCGGCGCTCGGCTCTTCGCTCGGC
GACGACGATGATGAGATGGGGGATCTGGACGTCTCTACCGGGGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGTGATGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCGGACA
TCCATTTCGAGCCGTATGAAGACGACTACCGGGTGCGGTTGCGGATCGATGGGCTGTTGAAGAACGTGGCCAAGGCGCCG
GTGAAGCTCAACCAGCGCATCGCGGCGCGCCTGAAGGTGATGTCGCAGCTGGACATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGTGTGAGCACCTTGCCAACCCTGTTCGGCGAGA
AGGTGGTACTGCGTATCCTGGACGCCAGTGCGGCCAAGCTTGGTATCGAGAAGCTGGGCTATGAGGCGGACCAACAGAAG
CTGTTCCTGGATGCGATACACAAGCCCTACGGCATGGTGCTGGTGACCGGGCCGACCGGTTCGGGCAAGACGGTGTCGCT
GTATACCGCGCTCGGGATTCTCAACGACGAGACGCGCAATATTTCCACGGCCGAGGATCCGGTTGAAATCCGCTTGCCTG
GTGTGAATCAGGTGCAGCAGAACGTCAAGCGCGGTATGACCTTCGCGGCGGCGCTGCGCTCGTTCCTGCGCCAGGATCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACCGGCCACATGGT
TCTGTCGACACTGCACACCAACGATGCGCCGCAAACCATTGCGCGCCTGATGAACATGGGCATCGCGCCGTACAACATCA
CCTCGTCGGTGACTCTGGTCATCGCGCAGCGCTTGGCGCGGCGCTTGTGCAACAACTGCAAGCGCAAGTCGACGCTGCCG
GAACATGCGCTGCTGGCGGAAGGCTTTACGCCGGAGCAGGTTGCGAACATCGAGCTCTATGAAGCGGTCGGTTGCGATGA
GTGCACGGAAGGCTACAAGGGCCGTACCGGCATCTATCAGGTGATGCCGATGACGGACGAAATCGGCGCGATCGTGCTGG
AAGGCGGCAATGCGATGCAGATCGCCGAGGCCGCACAGAAGATCGGTATCCGCGACCTGCGCCAGTCGGCGTTGATGAAG
GCTTCGCACGGGGTGACCAGCCTGGCCGAAATCAATCGTGTGACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8P673

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.162

98.44

0.553
  pilB Acinetobacter baylyi ADP1

55.458

98.44

0.546
  pilB Legionella pneumophila strain ERS1305867

51.937

98.44

0.511
  pilF Neisseria gonorrhoeae MS11

48.415

98.44

0.477
  pilB Vibrio cholerae strain A1552

49.002

95.494

0.468
  pilB Vibrio campbellii strain DS40M4

45.694

98.614

0.451
  pilB Vibrio parahaemolyticus RIMD 2210633

45.471

97.574

0.444