Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   H5647_RS02440 Genome accession   NZ_CP060092
Coordinates   602512..603546 (-) Length   344 a.a.
NCBI ID   WP_045856031.1    Uniprot ID   -
Organism   Teredinibacter purpureus strain Bs12     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 597512..608546
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H5647_RS02410 pilG 597625..598020 (-) 396 WP_045856025.1 twitching motility response regulator PilG Regulator
  H5647_RS02415 gshB 598306..599232 (+) 927 WP_408034019.1 glutathione synthase -
  H5647_RS02420 - 599240..600115 (+) 876 WP_045856027.1 energy transducer TonB -
  H5647_RS02425 - 600219..600824 (+) 606 WP_045856028.1 YqgE/AlgH family protein -
  H5647_RS02430 ruvX 600817..601260 (+) 444 WP_045856029.1 Holliday junction resolvase RuvX -
  H5647_RS02435 pilU 601275..602417 (-) 1143 WP_045856030.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  H5647_RS02440 pilT 602512..603546 (-) 1035 WP_045856031.1 type IV pilus twitching motility protein PilT Machinery gene
  H5647_RS02445 - 603673..604368 (+) 696 WP_045856032.1 YggS family pyridoxal phosphate-dependent enzyme -
  H5647_RS02450 - 604386..604958 (+) 573 WP_045856033.1 lipoprotein -
  H5647_RS02455 proC 605025..605870 (+) 846 WP_045856034.1 pyrroline-5-carboxylate reductase -
  H5647_RS02460 - 605878..606465 (+) 588 WP_045856035.1 YggT family protein -
  H5647_RS02465 - 606616..607767 (+) 1152 WP_045856036.1 homoserine O-acetyltransferase -
  H5647_RS02470 metW 607767..608357 (+) 591 WP_045856037.1 methionine biosynthesis protein MetW -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38259.92 Da        Isoelectric Point: 6.5790

>NTDB_id=473918 H5647_RS02440 WP_045856031.1 602512..603546(-) (pilT) [Teredinibacter purpureus strain Bs12]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPMEHKQVHGLIYEIMNDKQRKDYEEFLETDFSFEVPGVAR
FRVNAFNQNRGSAAVFRTIPSKVLTMEELGMGQVFRDVSSVPRGLVLVTGPTGSGKSTTLAAMIDYINENKYHHVLTIED
PIEFVHESKKCLVNQREVHRDTHGFAEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
VDVFPANEKSMVRSMLSESLEAVVSQTLMKRAGGGRVAAHEIMRGTPAIRNLIREDKVAQMYSAIQTGSALGMQTMDQCL
ADLVERRIVNRDVAKEKAKMPESF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=473918 H5647_RS02440 WP_045856031.1 602512..603546(-) (pilT) [Teredinibacter purpureus strain Bs12]
ATGGATATTACTGAATTACTGGCTTTTTCCGCCAAACAGGGCGCTTCGGATTTACACCTTTCCGCAGGCCTTCCACCGAT
GATTCGTGTCGACGGGGATGTACGTCGAATTAACTTGCCGCCAATGGAGCATAAGCAGGTTCACGGATTAATCTATGAGA
TTATGAACGACAAACAGCGTAAGGACTACGAAGAGTTTCTGGAAACGGACTTTTCGTTCGAAGTTCCTGGCGTTGCGCGT
TTTCGAGTGAATGCATTTAATCAAAATCGCGGTTCTGCGGCGGTGTTTCGTACGATTCCCTCTAAAGTTCTCACAATGGA
AGAGTTAGGAATGGGGCAAGTCTTTCGCGATGTATCGTCGGTGCCGCGTGGTCTCGTATTGGTTACAGGCCCTACGGGTT
CGGGTAAATCTACAACGCTGGCGGCCATGATTGATTATATCAACGAGAATAAATACCACCACGTACTTACCATTGAAGAT
CCCATTGAATTTGTGCACGAAAGTAAAAAATGCCTAGTGAACCAGCGGGAAGTACACCGTGATACCCATGGTTTCGCCGA
AGCCTTGCGCTCTGCTTTACGAGAAGATCCCGATATTATTCTTGTGGGCGAGATGCGAGATCTTGAAACGATTAGGCTGG
CGCTGACGGCAGCCGAAACCGGGCATTTGGTTTTTGGCACATTGCACACTACTTCGGCGGCAAAAACCATCGACCGAATT
GTTGATGTTTTTCCTGCTAATGAGAAATCTATGGTGCGTTCGATGCTATCGGAATCGTTGGAAGCGGTTGTTTCACAGAC
GTTAATGAAGCGAGCTGGGGGCGGCAGAGTGGCCGCGCATGAAATTATGCGCGGTACACCGGCGATTCGAAACCTGATTC
GCGAAGATAAAGTGGCTCAAATGTACTCAGCAATTCAAACAGGTAGTGCATTGGGGATGCAAACGATGGATCAGTGTTTG
GCGGATCTCGTTGAGCGTCGAATTGTGAATCGGGATGTAGCTAAAGAAAAAGCGAAAATGCCAGAGAGTTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

87.209

100

0.872
  pilT Pseudomonas stutzeri DSM 10701

86.628

100

0.866
  pilT Acinetobacter nosocomialis M2

81.105

100

0.811
  pilT Acinetobacter baumannii D1279779

81.105

100

0.811
  pilT Acinetobacter baumannii strain A118

81.105

100

0.811
  pilT Acinetobacter baylyi ADP1

79.07

100

0.791
  pilT Legionella pneumophila strain Lp02

74.26

98.256

0.73
  pilT Legionella pneumophila strain ERS1305867

74.26

98.256

0.73
  pilT Neisseria meningitidis 8013

67.826

100

0.68
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.235

98.837

0.674
  pilT Vibrio cholerae strain A1552

68.235

98.837

0.674
  pilT Neisseria gonorrhoeae MS11

67.246

100

0.674
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.043

100

0.532
  pilU Pseudomonas stutzeri DSM 10701

41.358

94.186

0.39
  pilU Acinetobacter baylyi ADP1

37.283

100

0.375
  pilU Vibrio cholerae strain A1552

39.692

94.477

0.375