Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   H5647_RS02435 Genome accession   NZ_CP060092
Coordinates   601275..602417 (-) Length   380 a.a.
NCBI ID   WP_045856030.1    Uniprot ID   -
Organism   Teredinibacter purpureus strain Bs12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 596275..607417
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H5647_RS02400 - 596587..597126 (-) 540 WP_045856023.1 chemotaxis protein CheW -
  H5647_RS02405 pilH 597206..597568 (-) 363 WP_045856024.1 twitching motility response regulator PilH -
  H5647_RS02410 pilG 597625..598020 (-) 396 WP_045856025.1 twitching motility response regulator PilG Regulator
  H5647_RS02415 gshB 598306..599232 (+) 927 WP_408034019.1 glutathione synthase -
  H5647_RS02420 - 599240..600115 (+) 876 WP_045856027.1 energy transducer TonB -
  H5647_RS02425 - 600219..600824 (+) 606 WP_045856028.1 YqgE/AlgH family protein -
  H5647_RS02430 ruvX 600817..601260 (+) 444 WP_045856029.1 Holliday junction resolvase RuvX -
  H5647_RS02435 pilU 601275..602417 (-) 1143 WP_045856030.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  H5647_RS02440 pilT 602512..603546 (-) 1035 WP_045856031.1 type IV pilus twitching motility protein PilT Machinery gene
  H5647_RS02445 - 603673..604368 (+) 696 WP_045856032.1 YggS family pyridoxal phosphate-dependent enzyme -
  H5647_RS02450 - 604386..604958 (+) 573 WP_045856033.1 lipoprotein -
  H5647_RS02455 proC 605025..605870 (+) 846 WP_045856034.1 pyrroline-5-carboxylate reductase -
  H5647_RS02460 - 605878..606465 (+) 588 WP_045856035.1 YggT family protein -

Sequence

Protein


Download         Length: 380 a.a.        Molecular weight: 42317.48 Da        Isoelectric Point: 6.3734

>NTDB_id=473917 H5647_RS02435 WP_045856030.1 601275..602417(-) (pilU) [Teredinibacter purpureus strain Bs12]
MDLDRLLSLMVEKGASDLFITAGVPPSMKLHGKILPVTSTPMSPEKSRETVLSVMNEKQRKEFMETKELNFAISARGVGR
FRASAFYQRNLAGMVLRRIETQIPKIDDLGLPESIKELAMVKRGLVLFVGATGTGKSTSLAAMIGHRNRNSKGHIISIED
PIEFIHQHQGCVITQREVGIDTASFEVALKNTLRQAPDVILIGEVRSRETMDHAIAFAETGHLCLATLHANNANQALDRI
IHFFPADRHSQLWMDLSLNLRAIVAQQLIPTPDGQGRRACLEIFLNTPLAQDLVRKGQVHELKELMKKSTELGMQTYDQA
LFELYDAGEITYEDALSHADSPNDLRLMIKLGSESDADYLSNAADELTIQDDEGSRSRRF

Nucleotide


Download         Length: 1143 bp        

>NTDB_id=473917 H5647_RS02435 WP_045856030.1 601275..602417(-) (pilU) [Teredinibacter purpureus strain Bs12]
ATGGATTTAGATCGTTTGTTATCGTTGATGGTTGAAAAAGGCGCATCAGATTTGTTTATTACTGCAGGTGTGCCGCCGTC
AATGAAGTTGCACGGCAAAATTTTGCCGGTAACCTCAACGCCAATGTCACCCGAAAAATCGCGTGAGACGGTGTTGAGCG
TGATGAACGAAAAGCAACGAAAGGAATTCATGGAAACAAAAGAGCTCAACTTCGCGATTAGCGCGCGCGGTGTGGGCCGT
TTTCGTGCCAGTGCATTTTATCAGCGTAATTTGGCTGGCATGGTTTTGCGGCGTATTGAAACTCAAATTCCTAAAATTGA
TGATTTGGGCCTGCCAGAATCTATCAAAGAACTTGCAATGGTTAAGCGTGGATTGGTGTTATTTGTGGGCGCCACGGGGA
CGGGTAAGTCGACGTCTTTGGCTGCCATGATTGGGCATCGAAATAGAAACTCTAAAGGCCATATTATTTCAATCGAAGAC
CCAATTGAGTTTATACATCAGCACCAGGGGTGTGTTATTACCCAGCGAGAGGTTGGTATTGATACCGCCTCGTTTGAGGT
CGCGCTGAAAAATACGCTACGCCAGGCTCCCGACGTTATCTTAATTGGGGAGGTTCGTTCGCGCGAAACGATGGATCATG
CCATTGCGTTTGCGGAAACAGGGCACCTCTGTTTGGCAACATTGCATGCGAATAATGCGAACCAAGCGTTGGATCGTATT
ATTCATTTTTTCCCTGCGGACAGGCATTCGCAGCTATGGATGGATTTATCGCTTAACTTGCGTGCAATTGTTGCACAGCA
ACTGATACCGACGCCCGACGGGCAAGGGCGAAGAGCCTGTTTGGAGATCTTTTTGAATACACCTCTAGCGCAGGATTTAG
TGCGTAAAGGGCAGGTGCATGAGTTGAAAGAGTTGATGAAAAAATCGACTGAACTGGGGATGCAAACATATGATCAGGCG
CTGTTTGAACTCTACGATGCGGGTGAAATTACGTATGAAGATGCACTTTCTCACGCAGATTCGCCCAATGATCTTCGGTT
GATGATTAAGTTAGGTTCTGAAAGTGACGCAGATTACCTCTCAAATGCGGCTGATGAGCTTACTATTCAGGACGATGAGG
GGAGCCGTTCTCGCCGTTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(76-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

77.953

100

0.782
  pilU Acinetobacter baylyi ADP1

66.387

93.947

0.624
  pilU Vibrio cholerae strain A1552

52.846

97.105

0.513
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.768

90.789

0.397
  pilT Legionella pneumophila strain Lp02

41.791

88.158

0.368
  pilT Legionella pneumophila strain ERS1305867

41.791

88.158

0.368
  pilT Pseudomonas aeruginosa PAK

40

90.789

0.363
  pilT Vibrio cholerae strain A1552

40.653

88.684

0.361
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.653

88.684

0.361
  pilT Pseudomonas stutzeri DSM 10701

39.71

90.789

0.361