Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   H5715_RS17075 Genome accession   NZ_CP060084
Coordinates   4196564..4197598 (+) Length   344 a.a.
NCBI ID   WP_075186257.1    Uniprot ID   -
Organism   Teredinibacter haidensis strain Bs08     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4191564..4202598
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H5715_RS17045 metW 4191823..4192413 (-) 591 WP_075186263.1 methionine biosynthesis protein MetW -
  H5715_RS17050 - 4192413..4193564 (-) 1152 WP_075186262.1 homoserine O-acetyltransferase -
  H5715_RS17055 - 4193711..4194298 (-) 588 WP_075186261.1 YggT family protein -
  H5715_RS17060 proC 4194307..4195131 (-) 825 WP_221892405.1 pyrroline-5-carboxylate reductase -
  H5715_RS17065 - 4195157..4195726 (-) 570 WP_075186259.1 hypothetical protein -
  H5715_RS17070 - 4195749..4196441 (-) 693 WP_075186258.1 YggS family pyridoxal phosphate-dependent enzyme -
  H5715_RS17075 pilT 4196564..4197598 (+) 1035 WP_075186257.1 type IV pilus twitching motility protein PilT Machinery gene
  H5715_RS17080 pilU 4197760..4198902 (+) 1143 WP_075186256.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  H5715_RS17085 ruvX 4199015..4199440 (-) 426 WP_075186255.1 Holliday junction resolvase RuvX -
  H5715_RS17090 - 4199433..4200017 (-) 585 WP_075186254.1 YqgE/AlgH family protein -
  H5715_RS17095 - 4200083..4200961 (-) 879 WP_075186253.1 TonB family protein -
  H5715_RS17100 gshB 4200970..4201926 (-) 957 WP_075186252.1 glutathione synthase -
  H5715_RS17105 pilG 4202163..4202558 (+) 396 WP_075186251.1 twitching motility response regulator PilG Regulator

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38193.92 Da        Isoelectric Point: 6.5070

>NTDB_id=473760 H5715_RS17075 WP_075186257.1 4196564..4197598(+) (pilT) [Teredinibacter haidensis strain Bs08]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPMEHKQVHGLIYEIMNDKQRKDYEEFLETDFSFEVPGVAR
FRVNAFNQNRGSGAVFRTIPSKVLTMEELGMGQVFRDVSSVPRGLVLVTGPTGSGKSTTLAAMIDFINENKYHHVLTIED
PIEFVHESKKCLVNQREVHRDTLGFAEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTVDRI
VDVFPANEKSMVRSMLSESLEAVVSQTLMKRTGGGRVAAHEIMRGTPAIRNLIREDKVAQMYSAIQTGGAIGMQTMDQCL
ADMVERRIIARDVAKEKAKMPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=473760 H5715_RS17075 WP_075186257.1 4196564..4197598(+) (pilT) [Teredinibacter haidensis strain Bs08]
ATGGATATTACTGAACTACTTGCTTTCTCCGCCAAGCAGGGTGCTTCTGATTTGCACTTGTCTGCTGGCCTACCGCCGAT
GATTCGTGTTGATGGCGATGTGCGTCGTATTAATTTGCCGCCGATGGAGCACAAGCAGGTTCACGGTCTGATCTACGAGA
TTATGAACGACAAGCAGCGTAAGGACTATGAAGAATTTCTGGAAACTGACTTTTCTTTCGAAGTTCCGGGTGTTGCCCGC
TTTCGTGTGAACGCATTTAATCAGAATCGTGGTTCTGGTGCAGTATTTCGTACAATTCCATCCAAGGTGCTTACAATGGA
GGAGTTGGGAATGGGGCAGGTATTCCGTGATGTCTCGTCTGTTCCGCGCGGGCTGGTCTTGGTTACTGGTCCAACGGGTT
CTGGCAAGTCCACAACTCTTGCAGCGATGATCGATTTTATCAACGAGAATAAATACCACCATGTTCTCACCATCGAAGAC
CCCATCGAATTTGTGCATGAGAGCAAAAAATGCCTGGTAAACCAGCGTGAAGTTCATCGCGACACCCTCGGTTTTGCCGA
AGCGCTGCGCTCTGCTTTGCGTGAAGACCCGGATATTATTCTGGTGGGTGAAATGCGAGATTTGGAAACGATTCGTTTGG
CGCTGACCGCAGCCGAAACAGGCCACCTAGTATTTGGCACCTTACACACCACTTCGGCGGCAAAAACCGTGGACCGCATT
GTGGACGTATTTCCGGCGAACGAAAAATCCATGGTGCGTTCCATGCTATCGGAATCGCTGGAAGCGGTTGTTTCGCAAAC
GTTGATGAAACGCACTGGCGGTGGTCGCGTAGCCGCGCACGAAATTATGCGTGGTACACCGGCTATTCGTAACCTTATTC
GCGAGGATAAAGTTGCGCAGATGTATTCTGCTATTCAAACCGGTGGAGCCATCGGTATGCAGACCATGGATCAGTGTTTG
GCCGATATGGTCGAACGCCGAATCATTGCTCGGGATGTGGCAAAAGAGAAAGCCAAGATGCCCGATAATTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

87.209

100

0.872
  pilT Pseudomonas stutzeri DSM 10701

86.628

100

0.866
  pilT Acinetobacter nosocomialis M2

79.942

100

0.799
  pilT Acinetobacter baumannii D1279779

79.942

100

0.799
  pilT Acinetobacter baumannii strain A118

79.942

100

0.799
  pilT Acinetobacter baylyi ADP1

78.198

100

0.782
  pilT Legionella pneumophila strain Lp02

73.669

98.256

0.724
  pilT Legionella pneumophila strain ERS1305867

73.669

98.256

0.724
  pilT Neisseria meningitidis 8013

68.529

98.837

0.677
  pilT Neisseria gonorrhoeae MS11

67.941

98.837

0.672
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.693

95.64

0.657
  pilT Vibrio cholerae strain A1552

68.693

95.64

0.657
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.043

100

0.532
  pilU Pseudomonas stutzeri DSM 10701

40.741

94.186

0.384
  pilU Acinetobacter baylyi ADP1

37.861

100

0.381
  pilU Vibrio cholerae strain A1552

39.385

94.477

0.372