Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   H5715_RS17080 Genome accession   NZ_CP060084
Coordinates   4197760..4198902 (+) Length   380 a.a.
NCBI ID   WP_075186256.1    Uniprot ID   -
Organism   Teredinibacter haidensis strain Bs08     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4192760..4203902
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H5715_RS17055 - 4193711..4194298 (-) 588 WP_075186261.1 YggT family protein -
  H5715_RS17060 proC 4194307..4195131 (-) 825 WP_221892405.1 pyrroline-5-carboxylate reductase -
  H5715_RS17065 - 4195157..4195726 (-) 570 WP_075186259.1 hypothetical protein -
  H5715_RS17070 - 4195749..4196441 (-) 693 WP_075186258.1 YggS family pyridoxal phosphate-dependent enzyme -
  H5715_RS17075 pilT 4196564..4197598 (+) 1035 WP_075186257.1 type IV pilus twitching motility protein PilT Machinery gene
  H5715_RS17080 pilU 4197760..4198902 (+) 1143 WP_075186256.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  H5715_RS17085 ruvX 4199015..4199440 (-) 426 WP_075186255.1 Holliday junction resolvase RuvX -
  H5715_RS17090 - 4199433..4200017 (-) 585 WP_075186254.1 YqgE/AlgH family protein -
  H5715_RS17095 - 4200083..4200961 (-) 879 WP_075186253.1 TonB family protein -
  H5715_RS17100 gshB 4200970..4201926 (-) 957 WP_075186252.1 glutathione synthase -
  H5715_RS17105 pilG 4202163..4202558 (+) 396 WP_075186251.1 twitching motility response regulator PilG Regulator
  H5715_RS17110 pilH 4202613..4202975 (+) 363 WP_075186250.1 twitching motility response regulator PilH -
  H5715_RS17115 - 4203050..4203589 (+) 540 WP_075186249.1 chemotaxis protein CheW -

Sequence

Protein


Download         Length: 380 a.a.        Molecular weight: 42473.65 Da        Isoelectric Point: 6.0959

>NTDB_id=473761 H5715_RS17080 WP_075186256.1 4197760..4198902(+) (pilU) [Teredinibacter haidensis strain Bs08]
MDFDRLLSLMVEKGASDLFITAGVPPSMKLHGKIVPVTSTPMSPEKSRETVLSVMNEKQRKEFMETKELNFAISARGVGR
FRASAFYQRNLAGMVLRRIETTIPKIDDLGLPEVIKELAMVKRGLVLFVGATGTGKSTSLAAMVGHRNRNSRGHIISIED
PIEFIHQHEGCVITQREVGIDTESFEVALKNTLRQAPDVILIGEVRARETMDHAIAFAETGHLCLCTLHANNANQALDRI
IHFFPADRHSQLWMDLSLNLRAIVAQQLIPTPDGMGRRACLEVFLNTPLAQDLIRKGNVHELKELMKKSTELGMQTFDQA
LYDLYDSGEITYEDALSHADSPNDLRLMIKLGSESDAEYLSNAADELSIQEDESNRSRRF

Nucleotide


Download         Length: 1143 bp        

>NTDB_id=473761 H5715_RS17080 WP_075186256.1 4197760..4198902(+) (pilU) [Teredinibacter haidensis strain Bs08]
ATGGATTTTGATCGTTTGTTGTCATTGATGGTAGAAAAAGGCGCTTCGGATTTATTTATTACCGCTGGCGTGCCACCTTC
TATGAAGCTTCACGGTAAGATTGTTCCGGTGACTTCCACACCGATGTCTCCGGAAAAATCCCGCGAAACGGTGTTGAGCG
TAATGAACGAAAAACAGCGCAAGGAGTTTATGGAAACCAAGGAGCTTAACTTCGCGATCAGTGCGCGAGGAGTTGGGCGC
TTTCGTGCCAGTGCTTTCTATCAGCGCAATTTGGCCGGTATGGTCTTGCGACGAATTGAAACGACGATCCCAAAAATTGA
TGACCTCGGCTTACCCGAAGTGATTAAAGAGCTAGCTATGGTTAAACGAGGGCTGGTGTTGTTTGTGGGTGCGACCGGCA
CGGGTAAATCGACTTCGTTGGCGGCGATGGTGGGCCACCGCAACCGTAATTCCAGGGGGCACATTATCTCCATCGAAGAC
CCGATCGAATTTATTCACCAGCATGAGGGTTGTGTGATTACCCAGCGCGAAGTGGGAATTGACACGGAATCATTTGAGGT
GGCGCTGAAAAACACTTTGCGGCAGGCCCCAGACGTGATTCTGATAGGCGAGGTTCGCGCCCGGGAAACGATGGATCACG
CTATTGCCTTTGCAGAAACTGGTCATCTTTGCTTGTGTACCCTTCATGCCAACAACGCCAACCAGGCGTTGGATCGCATT
ATTCACTTCTTCCCCGCTGACAGGCACTCGCAATTGTGGATGGATTTGTCGCTGAATCTGCGAGCTATTGTTGCTCAACA
GCTAATTCCTACGCCGGACGGTATGGGCCGTCGTGCCTGTCTGGAGGTTTTCCTGAATACCCCCCTGGCACAGGATTTAA
TTCGCAAAGGCAATGTGCATGAGCTGAAAGAGCTGATGAAAAAGTCCACAGAGCTGGGTATGCAGACCTTTGATCAGGCG
CTATACGATTTGTACGATTCCGGTGAGATTACCTACGAAGATGCGCTCTCCCATGCAGATTCGCCCAACGATCTACGCTT
GATGATAAAGTTAGGCTCTGAAAGCGATGCCGAATATTTATCCAACGCGGCAGATGAATTGAGTATTCAGGAGGATGAAT
CCAATCGTTCTAGGCGCTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(92-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

77.895

100

0.779
  pilU Acinetobacter baylyi ADP1

66.106

93.947

0.621
  pilU Vibrio cholerae strain A1552

55.143

92.105

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.188

90.789

0.392
  pilT Pseudomonas aeruginosa PAK

40.29

90.789

0.366
  pilT Vibrio cholerae strain A1552

40.95

88.684

0.363
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.95

88.684

0.363
  pilT Pseudomonas stutzeri DSM 10701

40

90.789

0.363
  pilT Legionella pneumophila strain ERS1305867

41.194

88.158

0.363
  pilT Legionella pneumophila strain Lp02

41.194

88.158

0.363