Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   GWK36_RS03695 Genome accession   NZ_CP048029
Coordinates   732385..733446 (+) Length   353 a.a.
NCBI ID   WP_166270008.1    Uniprot ID   A0A6G7VAZ7
Organism   Caldichromatium japonicum strain No.7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 727385..738446
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GWK36_RS03670 (GWK36_03670) - 727826..728164 (-) 339 WP_166270003.1 P-II family nitrogen regulator -
  GWK36_RS03675 (GWK36_03675) - 728190..728909 (-) 720 WP_166270004.1 TorF family putative porin -
  GWK36_RS03680 (GWK36_03680) - 729283..729549 (+) 267 WP_166270005.1 accessory factor UbiK family protein -
  GWK36_RS03685 (GWK36_03685) comM 729553..731067 (+) 1515 WP_166270006.1 YifB family Mg chelatase-like AAA ATPase Machinery gene
  GWK36_RS03690 (GWK36_03690) pilU 731171..732370 (+) 1200 WP_166270007.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GWK36_RS03695 (GWK36_03695) pilU 732385..733446 (+) 1062 WP_166270008.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GWK36_RS03700 (GWK36_03700) - 733625..735019 (-) 1395 WP_166270009.1 SUMF1/EgtB/PvdO family nonheme iron enzyme -
  GWK36_RS03705 (GWK36_03705) - 735164..736474 (+) 1311 WP_166270010.1 DUF3422 domain-containing protein -
  GWK36_RS03710 (GWK36_03710) - 736745..737434 (+) 690 WP_166270011.1 transposase -
  GWK36_RS03715 (GWK36_03715) - 737469..738017 (-) 549 WP_246237656.1 TIGR02281 family clan AA aspartic protease -

Sequence

Protein


Download         Length: 353 a.a.        Molecular weight: 39027.10 Da        Isoelectric Point: 6.4324

>NTDB_id=419573 GWK36_RS03695 WP_166270008.1 732385..733446(+) (pilU) [Caldichromatium japonicum strain No.7]
MDIKPYLELMVRHKASDLFFAPGAPVKIKIEGVIRSVGESTLTAALCREVVEGIMTPEQVETFRRELELDFAIALEGRAR
FRVNAYFQRGQPAMVLRYIRAEIPTLEELNAPPVLSQLIMHKRGIILMVGATGSGKSTTLAAMINHRNEHSTGHIVTIED
PIEFTHPHKQCLISQREIGIDTKSYAAALKSVLREAPDVVLIGEIRDRETMEAAIELAGTGHLAVSTLHANNAYQAMERI
VNLFPADRRKTLFMDLAVYLRAILSQRLVRTTAGTRCAAIEVMISTPHIADLIREGRIEELSQAMQETGRSEGMCTFDDA
LLALYQAGTISLEEALANADSRANLEAKIHFGA

Nucleotide


Download         Length: 1062 bp        

>NTDB_id=419573 GWK36_RS03695 WP_166270008.1 732385..733446(+) (pilU) [Caldichromatium japonicum strain No.7]
ATGGACATCAAGCCCTATCTCGAACTCATGGTCCGGCACAAGGCATCGGACCTGTTTTTTGCCCCGGGTGCGCCGGTCAA
GATCAAGATCGAGGGTGTCATCCGTTCGGTCGGCGAATCCACCCTGACCGCTGCCCTCTGTCGCGAGGTCGTCGAAGGGA
TTATGACCCCTGAGCAGGTCGAGACCTTTAGGCGCGAACTCGAGCTCGATTTTGCCATCGCCCTCGAGGGGCGGGCGCGC
TTCCGGGTCAATGCCTATTTCCAGCGCGGCCAGCCGGCGATGGTGTTGCGCTATATCCGCGCCGAGATCCCAACCCTTGA
GGAACTCAATGCCCCACCGGTCTTATCCCAGCTCATCATGCATAAGCGCGGGATCATCCTGATGGTTGGGGCCACGGGTT
CGGGCAAATCGACCACCCTGGCGGCCATGATCAATCACCGCAATGAGCATTCAACAGGACATATCGTCACCATCGAGGAC
CCAATCGAGTTCACTCATCCGCACAAACAATGCCTGATCAGCCAGCGCGAGATCGGGATCGACACCAAGAGCTATGCCGC
GGCCCTCAAGAGCGTCCTACGTGAGGCGCCGGATGTGGTCCTGATCGGCGAGATCCGCGACCGCGAGACCATGGAGGCGG
CGATCGAGTTGGCGGGTACCGGTCACCTAGCGGTCTCGACCCTGCACGCCAATAACGCCTATCAAGCCATGGAGCGCATC
GTCAATCTCTTCCCTGCGGATCGGCGCAAGACCCTATTCATGGACCTTGCGGTCTATCTGCGCGCCATCCTCTCGCAGCG
TCTGGTGCGCACCACTGCGGGTACCCGCTGTGCGGCGATCGAGGTCATGATCAGTACCCCGCACATCGCCGACCTCATCC
GCGAAGGTCGCATCGAGGAACTCAGCCAGGCCATGCAGGAGACCGGGCGCAGTGAGGGGATGTGCACCTTCGACGACGCC
CTGCTTGCGCTCTATCAGGCCGGCACCATCAGCCTGGAAGAGGCCCTGGCCAACGCCGATTCGCGCGCCAACCTCGAGGC
CAAGATCCATTTCGGTGCCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6G7VAZ7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

50.565

100

0.507
  pilU Acinetobacter baylyi ADP1

50.427

99.433

0.501
  pilU Vibrio cholerae strain A1552

48.563

98.584

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.765

96.317

0.402
  pilT Neisseria gonorrhoeae MS11

42.262

95.184

0.402
  pilT Neisseria meningitidis 8013

42.262

95.184

0.402
  pilT Pseudomonas aeruginosa PAK

41.159

97.734

0.402
  pilT Vibrio cholerae strain A1552

41.916

94.618

0.397
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.916

94.618

0.397
  pilT Pseudomonas stutzeri DSM 10701

40.29

97.734

0.394
  pilT Acinetobacter baylyi ADP1

40.762

96.601

0.394
  pilT Acinetobacter baumannii strain A118

40.896

94.901

0.388
  pilT Acinetobacter nosocomialis M2

40.896

94.901

0.388
  pilT Acinetobacter baumannii D1279779

40.896

94.901

0.388
  pilT Legionella pneumophila strain ERS1305867

41.088

93.768

0.385
  pilT Legionella pneumophila strain Lp02

41.088

93.768

0.385

Multiple sequence alignment