Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   GWK36_RS03690 Genome accession   NZ_CP048029
Coordinates   731171..732370 (+) Length   399 a.a.
NCBI ID   WP_166270007.1    Uniprot ID   A0A6G7VBL1
Organism   Caldichromatium japonicum strain No.7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 726171..737370
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GWK36_RS03665 (GWK36_03665) - 726453..727802 (-) 1350 WP_166270002.1 ammonium transporter -
  GWK36_RS03670 (GWK36_03670) - 727826..728164 (-) 339 WP_166270003.1 P-II family nitrogen regulator -
  GWK36_RS03675 (GWK36_03675) - 728190..728909 (-) 720 WP_166270004.1 TorF family putative porin -
  GWK36_RS03680 (GWK36_03680) - 729283..729549 (+) 267 WP_166270005.1 accessory factor UbiK family protein -
  GWK36_RS03685 (GWK36_03685) comM 729553..731067 (+) 1515 WP_166270006.1 YifB family Mg chelatase-like AAA ATPase Machinery gene
  GWK36_RS03690 (GWK36_03690) pilU 731171..732370 (+) 1200 WP_166270007.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GWK36_RS03695 (GWK36_03695) pilU 732385..733446 (+) 1062 WP_166270008.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GWK36_RS03700 (GWK36_03700) - 733625..735019 (-) 1395 WP_166270009.1 SUMF1/EgtB/PvdO family nonheme iron enzyme -
  GWK36_RS03705 (GWK36_03705) - 735164..736474 (+) 1311 WP_166270010.1 DUF3422 domain-containing protein -

Sequence

Protein


Download         Length: 399 a.a.        Molecular weight: 44199.56 Da        Isoelectric Point: 5.9378

>NTDB_id=419572 GWK36_RS03690 WP_166270007.1 731171..732370(+) (pilU) [Caldichromatium japonicum strain No.7]
MDRQQAANFMLECLRKMMEKNGSDLFISAGFPPACKINGRMTPMSSQPLTGEQTNMLVRSIMNDRQVREFDAHKECNFAI
NPKGIGRFRVNAFVQQGQSGAVLRTINSQIPSIDELHLPPILKEIAMTKRGLVLVVGATSSGKSTTLAAMIGHRNANSYG
HIVTIEDPVEYVHPHGHCLVTQREVGVDTDSWDAALVNTLRQAPDVILIGEIRTRETMEHAINFAETGHLCLSTLHANNA
NQALDRIINLFPEERRGQLLMDLSFNLKAIISQRLLPCTQGGRIPAVEIMLNSPLIQDLIFKGDVGGIKEVMERSRELGM
QTFDMALFDLYEAGKISYEDALRNADSMNALRLKIKLQGRDAHEKDHFGAAEGLSILGDEAEEEPEPNPFGTSPFKPES

Nucleotide


Download         Length: 1200 bp        

>NTDB_id=419572 GWK36_RS03690 WP_166270007.1 731171..732370(+) (pilU) [Caldichromatium japonicum strain No.7]
ATGGATCGCCAACAGGCCGCTAACTTCATGCTCGAATGTCTGCGCAAGATGATGGAAAAGAACGGCTCGGACCTCTTCAT
CTCCGCTGGCTTTCCGCCTGCTTGCAAGATCAATGGGCGCATGACCCCGATGAGCAGTCAGCCCTTGACCGGCGAACAGA
CCAATATGCTGGTGCGTTCGATCATGAACGACCGTCAAGTGCGTGAGTTCGACGCCCACAAGGAGTGCAACTTTGCGATC
AACCCCAAGGGGATTGGGCGCTTTCGCGTCAATGCCTTTGTCCAGCAGGGTCAATCCGGGGCCGTATTGCGTACCATCAA
TTCGCAGATCCCCAGCATCGATGAACTGCATTTGCCGCCGATCTTGAAAGAGATCGCGATGACCAAGCGCGGCCTGGTAT
TGGTCGTCGGCGCCACGAGTTCAGGCAAATCGACGACCCTTGCCGCTATGATCGGGCATCGCAATGCTAATAGCTATGGG
CACATCGTCACCATCGAGGACCCGGTCGAATATGTCCATCCGCATGGTCACTGTCTGGTGACCCAGCGCGAGGTGGGCGT
GGATACCGACAGCTGGGACGCGGCACTGGTCAATACCTTGCGCCAAGCGCCCGATGTGATCCTGATCGGCGAGATCCGTA
CCCGTGAGACCATGGAGCATGCGATCAACTTCGCCGAGACCGGTCACCTGTGCCTGTCCACCCTGCATGCCAATAACGCC
AACCAGGCGCTCGACCGCATCATCAACCTCTTCCCCGAGGAAAGGCGCGGCCAACTCCTGATGGATCTTTCCTTCAATCT
GAAGGCGATCATCTCTCAGCGCCTCTTGCCCTGTACCCAGGGCGGACGCATCCCGGCCGTAGAGATCATGCTCAACTCTC
CCTTGATCCAAGACCTGATCTTCAAGGGCGATGTCGGCGGGATCAAGGAGGTGATGGAGCGTTCGCGTGAGTTGGGGATG
CAAACCTTCGATATGGCCCTGTTCGATCTCTACGAGGCCGGCAAGATCAGCTATGAAGACGCCCTGCGCAACGCCGACTC
GATGAACGCCCTGCGGCTCAAGATCAAGCTCCAGGGGCGAGATGCGCATGAAAAGGATCATTTCGGTGCCGCTGAGGGGC
TGTCGATCCTGGGTGATGAGGCCGAAGAGGAGCCTGAGCCCAATCCTTTCGGCACGTCCCCGTTCAAGCCCGAATCCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(87-256)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6G7VBL1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.836

91.729

0.549
  pilU Acinetobacter baylyi ADP1

58.333

87.218

0.509
  pilU Vibrio cholerae strain A1552

52.691

88.471

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.51

84.461

0.376
  pilT Legionella pneumophila strain Lp02

43.939

82.707

0.363
  pilT Legionella pneumophila strain ERS1305867

43.939

82.707

0.363
  pilT Pseudomonas aeruginosa PAK

43.114

83.709

0.361

Multiple sequence alignment