Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SynSYN20_RS00095 Genome accession   NZ_CP047959
Coordinates   22056..23129 (-) Length   357 a.a.
NCBI ID   WP_186525332.1    Uniprot ID   A0A7G8J1D3
Organism   Synechococcus sp. SYN20     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 17056..28129
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SynSYN20_RS00070 (SynSYN20_00014) - 17326..17943 (+) 618 WP_186524228.1 RNA-binding protein -
  SynSYN20_RS00075 (SynSYN20_00015) dusA 17962..18966 (-) 1005 WP_186524229.1 tRNA dihydrouridine(20/20a) synthase DusA -
  SynSYN20_RS00080 (SynSYN20_00016) msrB 19035..19547 (+) 513 WP_186524230.1 peptide-methionine (R)-S-oxide reductase MsrB -
  SynSYN20_RS00085 (SynSYN20_00017) - 19474..20790 (+) 1317 WP_186524231.1 NAD(P)/FAD-dependent oxidoreductase -
  SynSYN20_RS00090 (SynSYN20_00018) - 20765..22039 (-) 1275 WP_186524232.1 type II secretion system F family protein -
  SynSYN20_RS00095 (SynSYN20_00019) pilT 22056..23129 (-) 1074 WP_186525332.1 type IV pilus twitching motility protein PilT Machinery gene
  SynSYN20_RS00100 (SynSYN20_00020) pilB/pilB1 23141..24913 (-) 1773 WP_370586690.1 GspE/PulE family protein Machinery gene
  SynSYN20_RS00105 (SynSYN20_00021) grpE 24912..25718 (+) 807 WP_186524234.1 nucleotide exchange factor GrpE -
  SynSYN20_RS00110 (SynSYN20_00022) dnaJ 25761..26891 (+) 1131 WP_186524235.1 molecular chaperone DnaJ -
  SynSYN20_RS00115 (SynSYN20_00023) - 26895..27137 (+) 243 WP_186524236.1 sulfurtransferase TusA family protein -
  SynSYN20_RS00120 (SynSYN20_00024) rsgA 27124..28038 (+) 915 WP_370586691.1 ribosome small subunit-dependent GTPase A -

Sequence

Protein


Download         Length: 357 a.a.        Molecular weight: 39325.73 Da        Isoelectric Point: 6.1087

>NTDB_id=418750 SynSYN20_RS00095 WP_186525332.1 22056..23129(-) (pilT) [Synechococcus sp. SYN20]
MERMIEDLMEQLVNGGGSDLHIASGQPPYGRFSGELRPMQEEPLSEEGCNRLIFSMLNNSQRKTLEQTWELDCAYGLKGV
ARFRVNVYRQKGSYAACLRALGSKIPSVELLNLPPVVVETSKRPRGLVLVTGPTGSGKTTTLAALLDHINHTRAEHILTI
EDPIEFVYTSDKSLVHQRQLNEDTRSFANALRAALREDPDVILVGEMRDLETIQLAISAAETGHLVFGTLHTSSAAQTVD
RMVDVFPPGQQTQIRVQLSGSLTAVFSQTLCRRQNPAPGQFGRVMAQEIMINTPAIANLIREGKTAQLYSQIQTGGERGM
QTLERALADLIDRGEISLDEGHSKASKPAELERLINN

Nucleotide


Download         Length: 1074 bp        

>NTDB_id=418750 SynSYN20_RS00095 WP_186525332.1 22056..23129(-) (pilT) [Synechococcus sp. SYN20]
ATGGAACGAATGATCGAAGACCTGATGGAACAGCTGGTGAACGGAGGCGGAAGCGACCTCCACATCGCCAGCGGACAACC
GCCTTACGGCCGCTTCAGTGGCGAACTGCGACCGATGCAAGAGGAACCGCTCAGTGAAGAAGGCTGCAACCGCCTGATCT
TTTCGATGCTCAATAACAGCCAACGCAAAACACTCGAACAAACGTGGGAGCTGGATTGTGCCTATGGCTTGAAAGGGGTC
GCCCGTTTTCGCGTGAATGTGTACCGCCAGAAGGGCAGCTATGCCGCCTGCTTGCGTGCTCTTGGGAGCAAAATCCCCAG
CGTTGAGCTGCTGAATCTGCCTCCAGTCGTCGTGGAAACCAGCAAACGCCCCCGTGGTCTTGTTCTGGTCACGGGCCCGA
CGGGTTCAGGCAAAACCACCACGCTCGCAGCACTTCTCGATCACATCAACCACACACGGGCTGAGCACATCCTCACCATC
GAAGACCCGATCGAATTCGTTTACACCAGTGATAAGAGCTTGGTGCATCAACGCCAACTCAACGAGGACACGCGCAGTTT
TGCCAATGCGCTGCGCGCTGCTCTGCGCGAAGACCCAGACGTGATTTTGGTGGGTGAAATGCGTGATCTCGAAACGATTC
AACTGGCAATCAGCGCTGCGGAAACTGGGCATTTGGTGTTTGGAACCTTGCACACCAGTTCTGCCGCTCAGACTGTTGAT
CGGATGGTGGATGTGTTTCCACCTGGCCAGCAAACCCAGATCCGCGTGCAACTCTCAGGAAGCCTGACGGCAGTGTTCTC
TCAAACGCTCTGCCGACGCCAAAATCCTGCACCTGGGCAGTTCGGTCGCGTGATGGCTCAAGAAATCATGATCAACACCC
CAGCCATCGCCAACCTGATCCGTGAAGGCAAAACAGCTCAGCTGTATTCCCAAATTCAAACCGGAGGCGAACGGGGCATG
CAAACCCTTGAAAGAGCGCTTGCCGATCTGATTGATCGCGGCGAAATCAGTCTTGATGAAGGGCACAGCAAAGCCAGCAA
ACCCGCGGAACTGGAACGCCTAATCAACAACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(121-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G8J1D3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

53.779

96.359

0.518
  pilT Pseudomonas stutzeri DSM 10701

53.779

96.359

0.518
  pilT Acinetobacter nosocomialis M2

51.744

96.359

0.499
  pilT Acinetobacter baumannii D1279779

51.744

96.359

0.499
  pilT Acinetobacter baumannii strain A118

51.744

96.359

0.499
  pilT Legionella pneumophila strain Lp02

52.199

95.518

0.499
  pilT Legionella pneumophila strain ERS1305867

52.199

95.518

0.499
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.858

98.88

0.493
  pilT Acinetobacter baylyi ADP1

50.872

96.359

0.49
  pilT Vibrio cholerae strain A1552

51.176

95.238

0.487
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.176

95.238

0.487
  pilT Neisseria meningitidis 8013

51.952

93.277

0.485
  pilT Neisseria gonorrhoeae MS11

50.733

95.518

0.485
  pilU Pseudomonas stutzeri DSM 10701

38.177

98.319

0.375
  pilU Vibrio cholerae strain A1552

37.644

97.479

0.367
  pilU Acinetobacter baylyi ADP1

37.356

97.479

0.364

Multiple sequence alignment