Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   SynSYN20_RS00100 Genome accession   NZ_CP047959
Coordinates   23141..24913 (-) Length   590 a.a.
NCBI ID   WP_370586690.1    Uniprot ID   -
Organism   Synechococcus sp. SYN20     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 18141..29913
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SynSYN20_RS00080 (SynSYN20_00016) msrB 19035..19547 (+) 513 WP_186524230.1 peptide-methionine (R)-S-oxide reductase MsrB -
  SynSYN20_RS00085 (SynSYN20_00017) - 19474..20790 (+) 1317 WP_186524231.1 NAD(P)/FAD-dependent oxidoreductase -
  SynSYN20_RS00090 (SynSYN20_00018) - 20765..22039 (-) 1275 WP_186524232.1 type II secretion system F family protein -
  SynSYN20_RS00095 (SynSYN20_00019) pilT 22056..23129 (-) 1074 WP_186525332.1 type IV pilus twitching motility protein PilT Machinery gene
  SynSYN20_RS00100 (SynSYN20_00020) pilB/pilB1 23141..24913 (-) 1773 WP_370586690.1 GspE/PulE family protein Machinery gene
  SynSYN20_RS00105 (SynSYN20_00021) grpE 24912..25718 (+) 807 WP_186524234.1 nucleotide exchange factor GrpE -
  SynSYN20_RS00110 (SynSYN20_00022) dnaJ 25761..26891 (+) 1131 WP_186524235.1 molecular chaperone DnaJ -
  SynSYN20_RS00115 (SynSYN20_00023) - 26895..27137 (+) 243 WP_186524236.1 sulfurtransferase TusA family protein -
  SynSYN20_RS00120 (SynSYN20_00024) rsgA 27124..28038 (+) 915 WP_370586691.1 ribosome small subunit-dependent GTPase A -
  SynSYN20_RS00125 (SynSYN20_00025) - 28013..28354 (-) 342 WP_186524237.1 YbaB/EbfC family nucleoid-associated protein -
  SynSYN20_RS00130 (SynSYN20_00026) murB 28379..29317 (-) 939 WP_186524238.1 UDP-N-acetylmuramate dehydrogenase -

Sequence

Protein


Download         Length: 590 a.a.        Molecular weight: 64090.08 Da        Isoelectric Point: 5.5550

>NTDB_id=418751 SynSYN20_RS00100 WP_370586690.1 23141..24913(-) (pilB/pilB1) [Synechococcus sp. SYN20]
MSADSTRMPLPPALPAKLISEAELAAGKALLHSGQVLDLETWKQLQALPIHLSDDGLMVAIASSSDRNSREQLKQVLKSH
GYISKLVLANGADLKQILDPQEFNSGSNISQTSISETAKSLLDGFDTEGILSADPDEAEIQSNSASSLDINSSITGASPV
VTLVDRILIKALDMNASDIHVEPQQAGLQIRLRKDGVLTNLAQPLPTKLIPAITSRFKIMADLDIAERRQAQDGRIRRQY
KGRTVDFRVNSLPSRYGEKICLRLLDSQSTQLGLDKLISNPNTLELVRTLGSKPFGMILVTGPTGSGKSTTLYSLLAERN
EPGINISTVEDPIEYTLPGITQCQVNREKGFDFSTALRAFMRQDPDVLLVGETRDQETAKTAIEAALTGHLVLTTLHCND
APSAIARLDEMGVEPFMVSASLLGIVSQRLLRRVCSDCRIPYHPDSRELGRFGLMTSDEGNVTFFKAKHHEGPETPCPTC
QGTGYKGRIGVYEVLRMNEALAASVAKGATTDLVRQLALEGGMKTLLGYSLDLVREGHTTLEEVDRMVLTDAGLESEQRA
RALSTVTCRGCGGGLQEGWLECPYCLTPRQ

Nucleotide


Download         Length: 1773 bp        

>NTDB_id=418751 SynSYN20_RS00100 WP_370586690.1 23141..24913(-) (pilB/pilB1) [Synechococcus sp. SYN20]
ATGAGCGCCGACTCCACCCGAATGCCGCTACCGCCTGCTCTGCCGGCAAAGCTGATTTCAGAGGCAGAGCTTGCCGCTGG
AAAAGCACTCCTCCATTCCGGGCAGGTTTTAGACCTTGAAACTTGGAAGCAACTTCAAGCGCTCCCCATCCATCTCAGCG
ACGACGGCCTCATGGTGGCCATCGCAAGCAGCAGCGACCGAAACAGTCGTGAACAACTCAAGCAGGTTTTAAAAAGCCAT
GGCTACATCTCCAAACTTGTACTAGCCAATGGAGCAGATCTAAAACAGATTCTTGATCCACAAGAATTCAACTCAGGTTC
AAATATCAGCCAAACATCAATTAGCGAGACAGCAAAATCGCTTCTCGATGGATTTGATACTGAAGGAATCTTAAGTGCCG
ATCCAGACGAAGCTGAAATTCAAAGCAATTCGGCCTCCTCGCTAGACATCAATTCATCAATAACCGGTGCATCTCCAGTC
GTCACATTGGTGGATCGTATCTTAATTAAGGCATTAGACATGAATGCCAGTGATATCCATGTAGAGCCGCAACAAGCAGG
ATTGCAAATTAGGCTTAGAAAGGATGGTGTACTCACCAATTTAGCGCAACCACTCCCCACTAAATTAATCCCAGCGATTA
CCTCTCGCTTCAAGATCATGGCTGATCTTGATATCGCTGAACGCAGGCAAGCACAAGATGGTCGCATCCGTAGACAATAC
AAAGGACGAACTGTTGACTTTCGCGTCAACAGCCTTCCCAGCCGCTATGGCGAAAAGATTTGCTTGCGGTTACTCGATAG
TCAATCCACTCAATTAGGACTGGATAAGCTCATCTCCAATCCCAACACACTCGAGCTTGTACGAACCCTTGGATCAAAAC
CATTCGGCATGATTCTTGTGACCGGTCCAACAGGATCTGGTAAATCGACAACTCTTTATTCGCTGTTAGCAGAGCGCAAC
GAACCCGGGATCAATATCTCCACCGTAGAAGATCCAATTGAATACACATTGCCTGGTATTACTCAATGTCAGGTCAATCG
AGAGAAAGGATTTGACTTCAGCACGGCCCTTCGCGCCTTCATGCGCCAGGACCCAGATGTTCTGTTAGTGGGTGAGACCC
GTGATCAAGAAACAGCGAAAACTGCTATCGAAGCAGCACTGACTGGGCACTTGGTCTTGACCACCCTGCACTGCAACGAT
GCACCGAGCGCCATCGCTCGACTCGACGAAATGGGCGTGGAACCGTTCATGGTGAGCGCATCCTTGCTCGGCATCGTTTC
CCAACGCTTGCTCAGACGCGTATGTAGTGATTGCCGGATCCCCTACCACCCAGATTCGCGAGAATTAGGCCGCTTTGGCC
TCATGACGAGCGATGAAGGCAATGTCACCTTTTTCAAAGCCAAACACCACGAAGGGCCAGAAACGCCATGCCCCACCTGT
CAGGGCACCGGTTACAAGGGCCGTATCGGTGTGTACGAGGTGCTGCGCATGAATGAAGCCTTAGCGGCATCTGTCGCCAA
AGGTGCAACCACTGATCTTGTCCGCCAGCTAGCTCTGGAAGGAGGCATGAAAACCTTGCTTGGCTACAGCCTCGATCTCG
TGCGAGAAGGTCACACCACTCTGGAAGAGGTGGACCGCATGGTTTTAACGGATGCGGGCCTGGAATCCGAACAACGCGCC
AGGGCCCTTAGCACTGTTACTTGCCGTGGATGTGGTGGCGGACTGCAAGAAGGTTGGCTGGAATGCCCGTATTGCCTGAC
ACCCCGTCAGTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(19-99)

Type II/IV secretion system protein (T2SSE)

(167-433)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

54.833

96.441

0.529

Multiple sequence alignment