Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AMBT_RS02025 Genome accession   NC_015554
Coordinates   476409..477452 (+) Length   347 a.a.
NCBI ID   WP_013782904.1    Uniprot ID   F5ZBN3
Organism   Alteromonas naphthalenivorans     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 471409..482452
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AMBT_RS02000 (ambt_02015) - 472667..473521 (-) 855 WP_013782899.1 hypothetical protein -
  AMBT_RS02005 (ambt_02020) - 473796..474236 (-) 441 WP_013782900.1 DUF4426 domain-containing protein -
  AMBT_RS02010 (ambt_02025) - 474259..474798 (-) 540 WP_013782901.1 YggT family protein -
  AMBT_RS02015 (ambt_02030) proC 474820..475641 (-) 822 WP_013782902.1 pyrroline-5-carboxylate reductase -
  AMBT_RS02020 (ambt_02035) - 475698..476384 (-) 687 WP_013782903.1 YggS family pyridoxal phosphate-dependent enzyme -
  AMBT_RS02025 (ambt_02040) pilT 476409..477452 (+) 1044 WP_013782904.1 type IV pilus twitching motility protein PilT Machinery gene
  AMBT_RS02030 (ambt_02045) pilU 477462..478574 (+) 1113 WP_013782905.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AMBT_RS02035 (ambt_02050) - 478580..478894 (+) 315 WP_013782906.1 DUF2007 domain-containing protein -
  AMBT_RS02040 (ambt_02055) ruvX 479273..479707 (-) 435 WP_013782907.1 Holliday junction resolvase RuvX -
  AMBT_RS02045 (ambt_02060) - 479700..480266 (-) 567 WP_013782908.1 YqgE/AlgH family protein -
  AMBT_RS02050 (ambt_02065) gshB 480309..481262 (-) 954 WP_013782909.1 glutathione synthase -
  AMBT_RS02055 (ambt_02070) - 481475..482203 (-) 729 WP_013782910.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38316.02 Da        Isoelectric Point: 6.5064

>NTDB_id=40717 AMBT_RS02025 WP_013782904.1 476409..477452(+) (pilT) [Alteromonas naphthalenivorans]
MDITELLAFSAKNKASDLHLSAGLPPLIRVDGEMRKLNVPALDHKQVHALIYEIMNDMQRKEYEENLETDFSFEISGLSR
FRVNAFVQNRGAAAVLRTIPNTVLTLDDLGAPEIFKEIINQPTGIVLVTGATGSGKSTTLAAMVDHINSHKREHILTIED
PIEFVHENKLSLVNQREVHRDTHSFSNALRSALREDPDVILVGELRDLETIRLAISAAETGHLVFGTLHTNSAPKTIDRI
IDVFPAEEKAMVRSMLSESLRAVISQTLLKKVGGGRIAAHEIMVGIPAIRNLIREDKVPQMYSVIQTGQATGMQTMDQCL
QRLLALGAISKEDAAAKSKDKQSMSNF

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=40717 AMBT_RS02025 WP_013782904.1 476409..477452(+) (pilT) [Alteromonas naphthalenivorans]
GTGGATATTACCGAACTTTTGGCTTTCAGCGCTAAAAATAAAGCGTCAGATTTGCACTTATCAGCAGGTCTGCCACCTCT
TATCCGCGTAGACGGAGAAATGCGTAAGCTTAATGTACCCGCATTAGATCATAAACAAGTCCATGCACTTATTTATGAAA
TAATGAATGACATGCAACGTAAAGAATACGAAGAGAACTTGGAAACCGATTTCTCATTCGAAATTAGTGGCTTGTCGCGG
TTTCGTGTAAATGCTTTTGTGCAAAATCGCGGTGCAGCAGCTGTGCTTCGTACTATCCCCAACACGGTTCTTACGCTTGA
TGATTTAGGTGCGCCTGAAATATTCAAAGAAATAATCAACCAACCTACCGGCATTGTTCTAGTCACTGGCGCCACAGGGT
CGGGTAAGAGTACAACCCTAGCTGCTATGGTTGACCATATCAATTCACATAAGCGCGAGCACATTCTTACTATCGAAGAT
CCTATCGAGTTTGTGCACGAAAATAAATTAAGTCTTGTAAACCAACGGGAAGTCCATCGAGACACCCACTCTTTCAGTAA
CGCACTTCGCTCTGCATTACGTGAAGATCCAGATGTTATTTTAGTTGGTGAATTACGTGATTTAGAGACCATCCGCTTAG
CTATTTCAGCAGCGGAAACTGGGCATTTAGTATTTGGCACCCTGCATACTAACTCAGCACCTAAAACAATAGACCGTATT
ATTGATGTGTTTCCTGCCGAAGAAAAAGCGATGGTGCGCTCTATGTTGTCTGAATCGCTAAGAGCTGTAATTTCTCAAAC
CCTGTTGAAGAAAGTAGGGGGAGGGCGAATCGCAGCGCATGAAATCATGGTAGGTATCCCTGCAATACGAAATCTTATTC
GTGAAGATAAAGTGCCTCAAATGTATTCGGTTATTCAAACGGGCCAAGCTACGGGTATGCAAACTATGGATCAGTGCCTA
CAACGCTTGTTGGCATTAGGTGCCATTTCTAAAGAAGACGCCGCTGCTAAATCGAAAGACAAGCAATCGATGAGTAATTT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F5ZBN3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

75.291

99.135

0.746
  pilT Pseudomonas aeruginosa PAK

75.516

97.695

0.738
  pilT Acinetobacter baylyi ADP1

73.256

99.135

0.726
  pilT Acinetobacter baumannii D1279779

73.256

99.135

0.726
  pilT Acinetobacter baumannii strain A118

73.256

99.135

0.726
  pilT Acinetobacter nosocomialis M2

72.965

99.135

0.723
  pilT Legionella pneumophila strain Lp02

73.054

96.254

0.703
  pilT Legionella pneumophila strain ERS1305867

73.054

96.254

0.703
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.217

97.118

0.692
  pilT Vibrio cholerae strain A1552

71.217

97.118

0.692
  pilT Neisseria meningitidis 8013

65.318

99.712

0.651
  pilT Neisseria gonorrhoeae MS11

65.029

99.712

0.648
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.846

93.66

0.504
  pilU Acinetobacter baylyi ADP1

42.262

96.83

0.409
  pilU Vibrio cholerae strain A1552

42.388

96.542

0.409
  pilU Pseudomonas stutzeri DSM 10701

41.194

96.542

0.398

Multiple sequence alignment