Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AMBT_RS02030 Genome accession   NC_015554
Coordinates   477462..478574 (+) Length   370 a.a.
NCBI ID   WP_013782905.1    Uniprot ID   F5ZBN4
Organism   Alteromonas naphthalenivorans     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 472462..483574
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AMBT_RS02000 (ambt_02015) - 472667..473521 (-) 855 WP_013782899.1 hypothetical protein -
  AMBT_RS02005 (ambt_02020) - 473796..474236 (-) 441 WP_013782900.1 DUF4426 domain-containing protein -
  AMBT_RS02010 (ambt_02025) - 474259..474798 (-) 540 WP_013782901.1 YggT family protein -
  AMBT_RS02015 (ambt_02030) proC 474820..475641 (-) 822 WP_013782902.1 pyrroline-5-carboxylate reductase -
  AMBT_RS02020 (ambt_02035) - 475698..476384 (-) 687 WP_013782903.1 YggS family pyridoxal phosphate-dependent enzyme -
  AMBT_RS02025 (ambt_02040) pilT 476409..477452 (+) 1044 WP_013782904.1 type IV pilus twitching motility protein PilT Machinery gene
  AMBT_RS02030 (ambt_02045) pilU 477462..478574 (+) 1113 WP_013782905.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AMBT_RS02035 (ambt_02050) - 478580..478894 (+) 315 WP_013782906.1 DUF2007 domain-containing protein -
  AMBT_RS02040 (ambt_02055) ruvX 479273..479707 (-) 435 WP_013782907.1 Holliday junction resolvase RuvX -
  AMBT_RS02045 (ambt_02060) - 479700..480266 (-) 567 WP_013782908.1 YqgE/AlgH family protein -
  AMBT_RS02050 (ambt_02065) gshB 480309..481262 (-) 954 WP_013782909.1 glutathione synthase -
  AMBT_RS02055 (ambt_02070) - 481475..482203 (-) 729 WP_013782910.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AMBT_RS22545 (ambt_02075) - 482350..482514 (-) 165 WP_013782911.1 hypothetical protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40858.58 Da        Isoelectric Point: 5.4057

>NTDB_id=40718 AMBT_RS02030 WP_013782905.1 477462..478574(+) (pilU) [Alteromonas naphthalenivorans]
MLDSFLEKMVEKSASDLFVTAGFPVSAKVHGKLTPISENSFNDEDALALVHEAMNEKQKEAFHTTKECNFAIVREGLGRF
RCSAFWQRDQAGMVVRRIVTEIPKVDDLGLPSVLKDIIMSKRGLVLFVGGTGTGKSTSLAALIGHRNSNSYGHILTIEDP
IEFVHEHKSCVITQREVGIDTHSFDDALKSSLRQAPDVILIGEIRSMETMEYAMSFADTGHLCVATLHANNANQAIERIM
HLAPKDQHDKLRFDLSQNIKAIVAQQLLPTKDGEGRVAAIEILLNSPLVSDLIQRNEIGSLKEAMKKGKELGMQSFDMAL
YDLYREGSIDLDQALHHADSPNDLRLMIKLDSNDGSSLGTLSNVSIDMDD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=40718 AMBT_RS02030 WP_013782905.1 477462..478574(+) (pilU) [Alteromonas naphthalenivorans]
ATGTTAGATAGCTTTTTAGAAAAAATGGTAGAGAAATCTGCTTCTGACTTATTCGTTACCGCGGGCTTTCCCGTTAGCGC
CAAAGTGCACGGTAAGCTTACTCCCATTTCGGAAAATTCATTTAATGATGAAGATGCATTAGCACTTGTTCATGAGGCTA
TGAATGAAAAGCAAAAAGAGGCTTTTCATACTACCAAGGAATGTAATTTTGCCATTGTACGAGAAGGCCTAGGGCGTTTT
CGTTGTTCGGCCTTCTGGCAACGAGATCAAGCCGGGATGGTGGTACGTCGTATTGTGACCGAAATTCCTAAGGTTGATGA
CCTGGGTTTACCTTCGGTGTTAAAAGATATCATTATGTCAAAGCGTGGTTTGGTACTTTTTGTAGGTGGCACAGGCACGG
GTAAATCAACCTCGTTGGCCGCACTTATCGGTCATCGAAATAGTAATTCTTATGGGCATATTTTAACTATTGAAGATCCT
ATTGAATTTGTGCATGAGCATAAATCTTGCGTGATAACTCAGCGTGAAGTGGGTATAGACACCCACTCATTCGACGATGC
ATTGAAAAGCTCCTTGCGACAAGCGCCTGATGTCATTCTTATTGGTGAAATTCGTTCAATGGAGACAATGGAATACGCCA
TGTCGTTCGCTGATACGGGGCACTTATGTGTGGCAACACTCCATGCCAACAACGCTAACCAAGCAATTGAGCGTATTATG
CATCTAGCGCCAAAAGACCAACATGACAAGTTACGTTTTGACTTAAGCCAAAATATAAAAGCTATAGTGGCACAACAACT
TCTTCCAACCAAAGACGGCGAGGGAAGGGTAGCGGCAATTGAAATACTATTAAACTCTCCTCTAGTGAGCGACCTTATTC
AACGCAATGAAATAGGTAGCTTGAAAGAAGCCATGAAAAAGGGCAAAGAGTTAGGCATGCAAAGTTTCGATATGGCGTTG
TACGATTTATATCGCGAGGGTAGTATTGATTTAGACCAAGCCTTACACCATGCTGATTCTCCCAATGATTTGCGCCTAAT
GATTAAGCTAGATTCTAACGATGGATCGAGCTTGGGCACATTATCGAATGTCTCAATCGATATGGATGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(105-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F5ZBN4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.299

100

0.595
  pilU Vibrio cholerae strain A1552

55.707

99.459

0.554
  pilU Acinetobacter baylyi ADP1

56.164

98.649

0.554
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.21

95.405

0.403
  pilT Acinetobacter baumannii strain A118

41.566

89.73

0.373
  pilT Acinetobacter nosocomialis M2

41.566

89.73

0.373
  pilT Acinetobacter baumannii D1279779

41.566

89.73

0.373
  pilT Legionella pneumophila strain Lp02

42.188

86.486

0.365
  pilT Legionella pneumophila strain ERS1305867

42.188

86.486

0.365
  pilT Acinetobacter baylyi ADP1

40.663

89.73

0.365
  pilT Pseudomonas stutzeri DSM 10701

40.419

90.27

0.365
  pilT Pseudomonas aeruginosa PAK

40.12

90.27

0.362

Multiple sequence alignment