Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   TSC_RS00750 Genome accession   NC_014974
Coordinates   137948..139036 (-) Length   362 a.a.
NCBI ID   WP_015716088.1    Uniprot ID   A0A430S5H3
Organism   Thermus scotoductus SA-01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 132948..144036
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TSC_RS00735 (TSC_c01510) - 134875..135504 (-) 630 WP_015716085.1 histidine phosphatase family protein -
  TSC_RS00740 (TSC_c01520) purM 135501..136502 (-) 1002 WP_015716086.1 phosphoribosylformylglycinamidine cyclo-ligase -
  TSC_RS00745 (TSC_c01530) gatB 136508..137917 (-) 1410 WP_015716087.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB -
  TSC_RS00750 (TSC_c01540) pilT 137948..139036 (-) 1089 WP_015716088.1 type IV pilus twitching motility protein PilT Machinery gene
  TSC_RS00755 (TSC_c01550) pilF 139048..141714 (-) 2667 WP_015716089.1 type IV pilus assembly ATPase PilB Machinery gene
  TSC_RS00760 (TSC_c01560) - 141711..142193 (-) 483 WP_015716090.1 YqeG family HAD IIIA-type phosphatase -
  TSC_RS00765 (TSC_c01580) pgeF 142228..142953 (-) 726 WP_015716091.1 peptidoglycan editing factor PgeF -
  TSC_RS00770 (TSC_c01570) - 143033..143437 (+) 405 WP_041437980.1 DUF4395 family protein -
  TSC_RS00775 (TSC_c01590) ribH 143424..143897 (-) 474 WP_015716093.1 6,7-dimethyl-8-ribityllumazine synthase -

Sequence

Protein


Download         Length: 362 a.a.        Molecular weight: 40057.32 Da        Isoelectric Point: 7.2367

>NTDB_id=39707 TSC_RS00750 WP_015716088.1 137948..139036(-) (pilT) [Thermus scotoductus SA-01]
MAKAPDIVDLLTLAADRGASDLVITVGLPPMIKVDGEFHPTEYETLSPQDTRRLMYALMDEKQQRIFEEEKELDFSFSLP
GKGRYRVNVFLQRGSVGGVLRVVPATIKSFEELGLPKNIAEIAMAPRGLVLVTGPTGSGKSTTLASMIDYINERKPVHIV
TIEDPIEFFHKHKKAIVNQREIGSDTHGFHKALRSVLRQAPDVILVGEMRDYETIAAAITAAETGHLVMGTLHTNSAPET
IDRIIDVFPENQQEQVRVQLSNNLVAVLTQQLLPKAFGGGRVLAYELMIATPAVRALIREGKSHQLRSVIQTGGQYGMIT
MDACLADLYRRKLITYEMGLSRAVDPKEFMRLAGVQEGSKRP

Nucleotide


Download         Length: 1089 bp        

>NTDB_id=39707 TSC_RS00750 WP_015716088.1 137948..139036(-) (pilT) [Thermus scotoductus SA-01]
ATGGCCAAAGCACCGGATATCGTGGATCTTCTGACCCTGGCGGCGGATCGGGGGGCCAGCGATTTGGTGATCACCGTGGG
CCTTCCCCCCATGATCAAGGTGGACGGGGAGTTCCACCCCACGGAGTACGAAACCCTCTCCCCCCAGGACACCCGCCGGC
TGATGTACGCCCTCATGGACGAGAAGCAGCAGCGGATCTTTGAGGAGGAAAAGGAGCTGGACTTCTCCTTCAGCCTCCCG
GGAAAAGGGCGCTACCGGGTGAACGTCTTCCTGCAACGGGGAAGCGTGGGAGGGGTCTTGAGGGTGGTGCCCGCCACCAT
CAAGAGCTTTGAGGAGCTGGGCCTGCCCAAGAACATCGCCGAGATCGCCATGGCCCCAAGGGGCCTGGTCCTGGTCACGG
GGCCCACCGGGTCGGGGAAGAGCACCACCCTGGCCTCCATGATCGACTACATCAACGAACGCAAGCCCGTGCACATCGTG
ACCATTGAGGATCCCATAGAGTTTTTCCACAAGCACAAGAAGGCCATCGTCAACCAGCGGGAGATCGGCTCCGATACCCA
CGGGTTCCACAAGGCCCTGAGAAGCGTTCTCCGCCAGGCCCCGGACGTGATCCTGGTGGGGGAGATGCGGGACTACGAGA
CCATCGCCGCCGCCATCACCGCCGCGGAAACCGGGCACTTGGTCATGGGGACCCTGCACACCAACTCCGCCCCCGAAACC
ATCGACCGCATCATCGACGTCTTCCCGGAAAACCAGCAGGAGCAGGTGCGGGTGCAGCTTTCCAACAACCTGGTGGCGGT
GCTCACCCAGCAGCTTCTGCCCAAGGCCTTCGGGGGCGGAAGGGTCTTGGCCTACGAGCTCATGATCGCCACCCCTGCGG
TAAGGGCCTTGATCCGGGAGGGCAAAAGCCACCAGCTCCGGAGCGTCATCCAGACCGGGGGCCAGTACGGGATGATCACC
ATGGATGCCTGCCTGGCGGATCTCTACCGGCGCAAGCTGATCACCTACGAGATGGGCTTGAGCCGGGCGGTGGACCCCAA
GGAGTTCATGCGCCTGGCCGGGGTGCAGGAGGGGAGCAAGCGCCCTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(15-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A430S5H3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

68.839

97.514

0.671
  pilT Pseudomonas stutzeri DSM 10701

54.36

95.028

0.517
  pilT Pseudomonas aeruginosa PAK

54.36

95.028

0.517
  pilT Acinetobacter baumannii strain A118

53.913

95.304

0.514
  pilT Acinetobacter nosocomialis M2

53.913

95.304

0.514
  pilT Acinetobacter baumannii D1279779

53.913

95.304

0.514
  pilT Acinetobacter baylyi ADP1

53.779

95.028

0.511
  pilT Neisseria meningitidis 8013

52.959

93.37

0.494
  pilT Neisseria gonorrhoeae MS11

52.226

93.094

0.486
  pilT Legionella pneumophila strain ERS1305867

51.163

95.028

0.486
  pilT Legionella pneumophila strain Lp02

51.163

95.028

0.486
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.28

90.884

0.475
  pilT Vibrio cholerae strain A1552

52.28

90.884

0.475
  pilU Pseudomonas stutzeri DSM 10701

46.647

94.751

0.442
  pilU Acinetobacter baylyi ADP1

41.341

98.895

0.409
  pilU Vibrio cholerae strain A1552

46.452

85.635

0.398

Multiple sequence alignment