Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   TSC_RS00755 Genome accession   NC_014974
Coordinates   139048..141714 (-) Length   888 a.a.
NCBI ID   WP_015716089.1    Uniprot ID   -
Organism   Thermus scotoductus SA-01     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 134048..146714
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TSC_RS00735 (TSC_c01510) - 134875..135504 (-) 630 WP_015716085.1 histidine phosphatase family protein -
  TSC_RS00740 (TSC_c01520) purM 135501..136502 (-) 1002 WP_015716086.1 phosphoribosylformylglycinamidine cyclo-ligase -
  TSC_RS00745 (TSC_c01530) gatB 136508..137917 (-) 1410 WP_015716087.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB -
  TSC_RS00750 (TSC_c01540) pilT 137948..139036 (-) 1089 WP_015716088.1 type IV pilus twitching motility protein PilT Machinery gene
  TSC_RS00755 (TSC_c01550) pilF 139048..141714 (-) 2667 WP_015716089.1 type IV pilus assembly ATPase PilB Machinery gene
  TSC_RS00760 (TSC_c01560) - 141711..142193 (-) 483 WP_015716090.1 YqeG family HAD IIIA-type phosphatase -
  TSC_RS00765 (TSC_c01580) pgeF 142228..142953 (-) 726 WP_015716091.1 peptidoglycan editing factor PgeF -
  TSC_RS00770 (TSC_c01570) - 143033..143437 (+) 405 WP_041437980.1 DUF4395 family protein -
  TSC_RS00775 (TSC_c01590) ribH 143424..143897 (-) 474 WP_015716093.1 6,7-dimethyl-8-ribityllumazine synthase -
  TSC_RS00780 (TSC_c01600) crcB 143918..144295 (+) 378 WP_038029344.1 fluoride efflux transporter CrcB -
  TSC_RS00785 (TSC_c01610) - 144300..144632 (+) 333 WP_015716095.1 DUF190 domain-containing protein -
  TSC_RS00790 (TSC_c01620) - 144677..145291 (+) 615 WP_015716096.1 sulfite oxidase-like oxidoreductase -
  TSC_RS00795 (TSC_c01630) - 145288..145557 (+) 270 WP_015716097.1 hypothetical protein -
  TSC_RS00800 (TSC_c01640) - 145533..146336 (-) 804 WP_041437982.1 histidinol-phosphatase HisJ family protein -

Sequence

Protein


Download         Length: 888 a.a.        Molecular weight: 97878.91 Da        Isoelectric Point: 5.0875

>NTDB_id=39708 TSC_RS00755 WP_015716089.1 139048..141714(-) (pilF) [Thermus scotoductus SA-01]
MSVLTIGDKRLGAILLDAGLLTDEELQMALEKHREVGGSLAEVIVDSGLLSERRIAQAIEDHFGIPLVELHTLEIPPKVK
ALLPAEKAKELQAIPFALDEEAGVVRVAFVNPLDTLALEEVEDLTGMVVEPYQATKSAFLYALAKNYPELNLPLPPPPSG
PSREELKVGELLVEKGLLDRNTLEEALVEQEKTGDLLGRILVRKGLPEEVLYRVLAEQKGMEFLPSTRGLSPDPAATNLL
LRSDALRYSAVPVGFNNGEVEVVLADPRHKEAVAELLGRPARFLLTLPKEWEALFHQAYPEKSRLGEVLVQEGRLSQEEL
REALEVQRRLPKAKPLGEILVELGLARPEDVEEALKKQRQGGGRLEDTLVASGKLKPEALAQAVAAQLGYTYINPEENPP
DPGAALLLPEDLARRYGVFPHHLEGKTLVLLMKDPRNILALDDVRLALKRKGLAYEVQPAVATEAAITKLIERFYGKEEL
GEIAKELSKGYQEEEAVAPELDESAAQKFVKQVIREAYLQDASDIHIEPRQSDVLVRLRIDGTLRQYTTLPKGALNSIIS
VVKIMGGLNIAERRLPQDGRVRYREGAIDVDLRLSTLPTVYGEKAVMRLLKKAADIPEIEGLGFAPGVFERFQEVISKPY
GIFLITGPTGSGKSFTTFSILKRIATPDKNTQTIEDPVEYEIPGINQTQVNPQAGLTFARALRAFLRQDPDIIMVGEIRD
SETAKIATEAALTGHLVIATLHTNDAAQAITRLDEMGVELFNISAALIGVLSQRLVRRICDHCKVEVKPDPEVLRRLGLS
QEEIQGAKLYKGMGCERCGGTGYKGRYAIHELLVVDDEIRHAIVAGKSATEIKEIARKKGMKTLREDGIYKAFQGITTLE
EVLARTIE

Nucleotide


Download         Length: 2667 bp        

>NTDB_id=39708 TSC_RS00755 WP_015716089.1 139048..141714(-) (pilF) [Thermus scotoductus SA-01]
ATGAGCGTGCTCACCATCGGGGACAAACGGCTAGGTGCCATCCTATTGGATGCCGGCCTCCTCACCGACGAGGAGCTGCA
GATGGCCCTGGAGAAGCACCGGGAGGTGGGGGGTTCCTTGGCCGAGGTCATCGTGGACTCGGGGCTGCTTTCGGAACGGC
GGATCGCTCAGGCCATCGAGGACCACTTCGGCATCCCCCTGGTGGAGCTTCACACCCTGGAGATCCCTCCCAAGGTGAAG
GCCCTCCTGCCGGCGGAGAAGGCCAAGGAACTCCAGGCCATCCCCTTCGCCCTGGACGAGGAGGCAGGGGTGGTGCGGGT
GGCCTTCGTGAACCCCCTGGACACCTTGGCCCTCGAGGAGGTGGAGGACCTCACCGGGATGGTGGTGGAACCCTACCAGG
CCACCAAAAGCGCCTTCCTCTACGCCCTGGCCAAGAACTACCCCGAGCTCAACCTGCCCCTACCCCCTCCCCCCAGCGGC
CCCAGCCGGGAGGAGCTCAAGGTGGGCGAGCTTCTGGTGGAAAAGGGCCTCCTGGACCGGAACACCCTGGAGGAAGCCCT
GGTGGAGCAGGAAAAGACCGGGGATCTCCTGGGCCGCATCCTGGTGCGCAAGGGGCTACCCGAGGAAGTCCTCTACCGGG
TTTTGGCCGAGCAGAAGGGAATGGAGTTTCTCCCCTCCACCCGGGGCCTATCCCCCGACCCCGCCGCCACCAACCTTCTC
CTGCGCTCGGATGCCCTGCGCTATAGCGCCGTGCCCGTGGGGTTCAACAACGGGGAGGTGGAGGTGGTCCTCGCCGATCC
CCGGCACAAGGAGGCGGTGGCGGAGCTCCTTGGGCGCCCCGCCCGCTTCCTCCTCACCCTTCCCAAGGAGTGGGAAGCCC
TTTTCCACCAGGCCTATCCGGAAAAAAGCCGCCTGGGGGAGGTCCTGGTCCAGGAGGGGCGCCTAAGCCAGGAGGAGTTA
AGGGAAGCCCTCGAGGTGCAAAGGCGCCTGCCCAAGGCCAAGCCCCTGGGGGAGATCCTGGTGGAGCTGGGCCTGGCCCG
GCCCGAGGACGTGGAGGAAGCCCTGAAGAAGCAACGCCAGGGTGGGGGCCGCCTCGAGGACACCTTGGTGGCCTCGGGCA
AACTCAAACCCGAGGCCCTGGCCCAGGCGGTGGCTGCCCAGCTGGGCTACACCTACATCAACCCCGAGGAGAACCCCCCG
GACCCCGGGGCCGCCCTCCTCCTCCCCGAGGACCTGGCCCGGCGGTACGGGGTCTTCCCCCACCACCTGGAGGGCAAGAC
CCTGGTCCTCCTCATGAAGGATCCCAGGAACATCCTGGCCCTGGACGACGTGCGCCTGGCCCTGAAGCGCAAGGGCCTGG
CCTACGAGGTTCAGCCCGCCGTGGCCACGGAGGCCGCCATCACCAAGCTCATCGAGCGCTTCTACGGCAAGGAGGAACTG
GGCGAGATCGCCAAGGAACTTTCCAAGGGCTACCAGGAGGAGGAGGCCGTCGCCCCCGAGCTGGACGAAAGCGCCGCCCA
GAAGTTCGTCAAGCAGGTGATCCGGGAAGCCTACCTGCAGGATGCCTCCGACATCCACATAGAACCCCGGCAGTCCGATG
TGCTGGTGCGCCTCCGCATCGACGGCACCCTGCGCCAGTACACCACCCTGCCCAAGGGGGCCCTAAACTCCATCATCAGC
GTGGTCAAGATCATGGGCGGCCTCAACATCGCCGAAAGGCGCCTGCCCCAGGACGGGCGGGTGCGCTACCGGGAAGGGGC
CATCGACGTGGACCTCCGCCTCTCCACCCTGCCCACGGTCTACGGGGAAAAGGCGGTGATGCGCCTTTTGAAGAAGGCCG
CCGACATCCCCGAGATCGAGGGCCTGGGCTTCGCTCCCGGGGTCTTTGAGCGCTTCCAGGAGGTGATCTCCAAGCCCTAC
GGCATCTTCCTCATCACCGGGCCCACCGGAAGCGGCAAAAGCTTCACCACCTTCTCCATCCTCAAGCGCATCGCCACCCC
AGACAAGAACACCCAGACCATCGAGGATCCCGTGGAGTACGAGATCCCCGGCATCAACCAGACCCAGGTGAACCCCCAGG
CGGGCCTCACCTTTGCCCGGGCCCTCAGGGCCTTCCTCAGGCAGGACCCGGACATCATCATGGTGGGAGAGATCCGGGAC
TCGGAAACCGCCAAGATCGCCACCGAGGCCGCCCTCACCGGCCACCTGGTCATCGCCACCCTGCACACCAACGACGCCGC
CCAGGCCATCACCCGCCTGGACGAGATGGGGGTGGAGCTTTTCAATATCTCCGCCGCCCTCATCGGGGTTCTCTCCCAGC
GCCTGGTGCGGAGGATCTGCGACCACTGCAAGGTGGAGGTGAAGCCCGACCCCGAGGTCCTGCGGCGCCTGGGCCTTTCC
CAGGAGGAGATCCAAGGGGCCAAACTCTACAAGGGGATGGGGTGCGAGCGCTGCGGGGGAACGGGCTATAAGGGACGCTA
CGCCATCCACGAACTCCTGGTGGTGGACGACGAGATCCGCCACGCCATCGTGGCCGGCAAGTCGGCCACGGAGATCAAGG
AGATCGCCAGGAAAAAGGGTATGAAAACCCTGCGGGAGGACGGCATCTACAAGGCCTTCCAGGGAATCACCACCCTCGAG
GAGGTCCTGGCACGTACCATTGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(512-778)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(173-303)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(369-479)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

87.514

100

0.876
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

56.404

100

0.565

Multiple sequence alignment