Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   GFH30_RS03165 Genome accession   NZ_CP045650
Coordinates   688204..689325 (-) Length   373 a.a.
NCBI ID   WP_153370864.1    Uniprot ID   -
Organism   Acinetobacter wanghuae strain dk386     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 683204..694325
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GFH30_RS03135 (GFH30_03140) - 683959..684807 (+) 849 WP_153370858.1 class II glutamine amidotransferase -
  GFH30_RS03140 (GFH30_03145) - 685075..685530 (+) 456 WP_153370859.1 bacteriohemerythrin -
  GFH30_RS03145 (GFH30_03150) - 685672..686751 (+) 1080 WP_153370860.1 hypothetical protein -
  GFH30_RS03150 (GFH30_03155) - 686751..687089 (+) 339 WP_153370861.1 RnfH family protein -
  GFH30_RS03155 (GFH30_03160) - 687181..687579 (-) 399 WP_153370862.1 outer membrane protein assembly factor BamE -
  GFH30_RS03160 (GFH30_03165) fur 687692..688129 (+) 438 WP_153370863.1 ferric iron uptake transcriptional regulator -
  GFH30_RS03165 (GFH30_03170) pilU 688204..689325 (-) 1122 WP_153370864.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GFH30_RS03170 (GFH30_03175) pilT 689353..690390 (-) 1038 WP_153370865.1 type IV pilus twitching motility protein PilT Machinery gene
  GFH30_RS03175 (GFH30_03180) - 690514..691203 (+) 690 WP_153370866.1 YggS family pyridoxal phosphate-dependent enzyme -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41654.72 Da        Isoelectric Point: 6.7811

>NTDB_id=395905 GFH30_RS03165 WP_153370864.1 688204..689325(-) (pilU) [Acinetobacter wanghuae strain dk386]
MDFNGLLDYMIEKKASDLFVTADVEPSIKINGQIHPIGSVKLPGSAVAQLVHSIMSDKQRREFAETRECNFAISNASQTA
RFRVSAFQQRDQPGMVLRRIETTIPTMDELKLPPVLKELAMTKRGIIIFVGATGTGKSTSLASLIGYRNENSKGHIITIE
DPIEFVHQHKGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVFATLHANNANQAIDR
IIHFFEADRHSQLFMDLSLNLKAMVAQQLIPTPDGTGRRAAIEVLINSPLISDLIRKGDVHEIKEIMKRSRELGMQTFDQ
ALYDLYKAKQITYKDALKHADSPNDLRLQIKLSEEGGTHLMNANSNITFDGQS

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=395905 GFH30_RS03165 WP_153370864.1 688204..689325(-) (pilU) [Acinetobacter wanghuae strain dk386]
ATGGATTTTAATGGCTTACTTGATTATATGATCGAGAAGAAAGCATCGGATTTGTTTGTTACCGCCGATGTTGAACCTTC
TATTAAAATTAATGGTCAAATTCATCCAATTGGATCGGTCAAACTTCCGGGTTCTGCGGTAGCACAATTGGTTCATTCAA
TTATGTCCGATAAGCAGCGCCGTGAATTTGCAGAAACACGCGAGTGTAACTTTGCCATTAGCAATGCCAGTCAAACCGCA
CGTTTTCGTGTGAGTGCATTTCAGCAACGTGATCAACCGGGCATGGTGTTACGTCGTATTGAAACCACTATTCCGACGAT
GGATGAACTGAAGTTACCGCCTGTTCTGAAAGAATTGGCGATGACCAAGCGCGGTATTATTATTTTTGTAGGTGCAACGG
GTACAGGTAAATCAACCTCATTGGCATCGCTCATTGGCTATCGTAATGAAAATTCCAAAGGTCATATCATTACCATTGAA
GATCCAATCGAGTTTGTACACCAACATAAAGGCTGTATTATCACCCAGCGTGAAGTAGGTATAGATACAGATTCATTTGA
AATTGCGTTAAAAAATACTTTGCGTCAGGCACCTGATGTGATTTTGATTGGTGAGATTCGTTCACGCGAAACGATGGATT
ATGCGATTGCCTTTGCAGAAACAGGTCACTTGGTATTTGCAACCCTGCATGCCAACAATGCCAACCAAGCGATTGATCGT
ATTATTCACTTCTTTGAAGCCGATCGTCATAGCCAGTTGTTTATGGATTTATCCTTAAACTTAAAAGCAATGGTGGCGCA
GCAACTGATTCCAACACCAGATGGTACAGGTCGCCGTGCAGCGATTGAAGTGTTGATCAATTCACCTTTAATTTCAGACT
TGATCCGTAAAGGTGACGTGCATGAAATTAAAGAAATTATGAAACGTTCACGCGAACTCGGGATGCAAACCTTTGACCAA
GCTTTATATGATCTCTATAAAGCCAAGCAAATCACGTACAAAGATGCATTGAAACATGCTGACTCGCCAAACGATTTACG
TCTGCAAATCAAACTGTCCGAAGAAGGTGGCACTCATCTCATGAATGCCAATTCAAATATCACCTTTGATGGTCAATCTT
AA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-250)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

73.28

100

0.743
  pilU Pseudomonas stutzeri DSM 10701

69.272

99.464

0.689
  pilU Vibrio cholerae strain A1552

55.676

99.196

0.552
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.353

92.761

0.402
  pilT Acinetobacter baumannii strain A118

41.983

91.957

0.386
  pilT Acinetobacter nosocomialis M2

41.983

91.957

0.386
  pilT Acinetobacter baumannii D1279779

41.983

91.957

0.386
  pilT Legionella pneumophila strain Lp02

42.56

90.08

0.383
  pilT Legionella pneumophila strain ERS1305867

42.56

90.08

0.383
  pilT Acinetobacter baylyi ADP1

40.816

91.957

0.375
  pilT Pseudomonas aeruginosa PAK

40.462

92.761

0.375
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.601

94.102

0.373
  pilT Vibrio cholerae strain A1552

39.601

94.102

0.373
  pilT Neisseria meningitidis 8013

39.595

92.761

0.367
  pilT Neisseria gonorrhoeae MS11

39.595

92.761

0.367
  pilT Pseudomonas stutzeri DSM 10701

39.595

92.761

0.367

Multiple sequence alignment