Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GFH30_RS03170 Genome accession   NZ_CP045650
Coordinates   689353..690390 (-) Length   345 a.a.
NCBI ID   WP_153370865.1    Uniprot ID   A0A5Q0P1R3
Organism   Acinetobacter wanghuae strain dk386     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 684353..695390
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GFH30_RS03140 (GFH30_03145) - 685075..685530 (+) 456 WP_153370859.1 bacteriohemerythrin -
  GFH30_RS03145 (GFH30_03150) - 685672..686751 (+) 1080 WP_153370860.1 hypothetical protein -
  GFH30_RS03150 (GFH30_03155) - 686751..687089 (+) 339 WP_153370861.1 RnfH family protein -
  GFH30_RS03155 (GFH30_03160) - 687181..687579 (-) 399 WP_153370862.1 outer membrane protein assembly factor BamE -
  GFH30_RS03160 (GFH30_03165) fur 687692..688129 (+) 438 WP_153370863.1 ferric iron uptake transcriptional regulator -
  GFH30_RS03165 (GFH30_03170) pilU 688204..689325 (-) 1122 WP_153370864.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GFH30_RS03170 (GFH30_03175) pilT 689353..690390 (-) 1038 WP_153370865.1 type IV pilus twitching motility protein PilT Machinery gene
  GFH30_RS03175 (GFH30_03180) - 690514..691203 (+) 690 WP_153370866.1 YggS family pyridoxal phosphate-dependent enzyme -
  GFH30_RS03180 (GFH30_03185) - 691260..694859 (-) 3600 WP_153370867.1 AAA family ATPase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38256.92 Da        Isoelectric Point: 6.5778

>NTDB_id=395906 GFH30_RS03170 WP_153370865.1 689353..690390(-) (pilT) [Acinetobacter wanghuae strain dk386]
MDITELLAFSAKNGASDLHLSAGMPPMIRVDGEVRRINLPVLEHKEVHKLVYDIMNDKQRRDFEEHLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTIEDLGLGQIFKDICEFPRGLVLVTGPTGSGKSTTLAALIDYINDNRYDHILTVED
PIEFVHQSKKCLVNQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANYGMTTLDQSL
KTLVSKGMISPQVARTAAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=395906 GFH30_RS03170 WP_153370865.1 689353..690390(-) (pilT) [Acinetobacter wanghuae strain dk386]
ATGGATATTACAGAATTATTAGCCTTTTCTGCTAAAAACGGCGCATCAGACTTACATTTATCTGCGGGTATGCCACCGAT
GATTCGGGTCGATGGTGAAGTTCGTCGTATTAATCTACCGGTATTAGAACACAAAGAAGTCCATAAGCTTGTTTATGACA
TCATGAATGATAAACAGCGTCGTGACTTCGAAGAACACTTAGAAACTGACTTTTCATTTGAAGTTCCGGGTGTGGCTCGT
TTCCGTGTGAACGCATTTAACCAAAATCGTGGCGCAGGTGCTGTATTTCGTACTATTCCATCAAAAGTACTCACCATTGA
AGATTTAGGTTTAGGTCAAATCTTTAAAGATATCTGTGAATTCCCGCGTGGCTTAGTTTTAGTGACCGGTCCAACTGGTT
CAGGTAAATCAACCACACTGGCGGCTTTGATTGATTACATTAATGACAACCGTTATGACCATATTTTAACGGTCGAAGAT
CCAATCGAGTTTGTACACCAATCAAAAAAATGTTTGGTCAACCAACGTGAAGTACACCGTGATACGCATGGCTTTAACGA
AGCACTGCGTTCAGCACTACGTGAAGACCCTGATATTATTCTTGTCGGTGAGATGCGTGACTTAGAAACCATTCGTTTGG
CGCTAACAGCTGCGGAAACGGGTCACTTGGTCTTCGGTACACTACATACCACTTCTGCCGCAAAAACTATTGACCGTGTG
ATTGACGTGTTCCCTGCTGAAGAAAAAGACATGGTTCGTGCCATGTTGTCTGAATCACTACAAGCCGTCATTTCACAAAC
CCTGCTCAAGAAAAATGGCGGTGGTCGTGTGGCAGCCCATGAAATCATGATTGGTATTCCTGCAATTCGTAACCTCATCC
GTGAAAACAAAGTTGCTCAGATGTATTCTGCAATTCAAACGGGTGCCAACTATGGTATGACCACCCTTGACCAAAGCTTA
AAAACCTTGGTGTCTAAGGGCATGATTAGCCCACAAGTGGCACGTACTGCGGCAAAACAGCCAGAATCATTCCTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5Q0P1R3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

94.203

100

0.942
  pilT Acinetobacter baumannii D1279779

93.913

100

0.939
  pilT Acinetobacter baumannii strain A118

93.913

100

0.939
  pilT Acinetobacter baylyi ADP1

86.047

99.71

0.858
  pilT Pseudomonas aeruginosa PAK

84.302

99.71

0.841
  pilT Pseudomonas stutzeri DSM 10701

83.721

99.71

0.835
  pilT Legionella pneumophila strain Lp02

75.581

99.71

0.754
  pilT Legionella pneumophila strain ERS1305867

75.581

99.71

0.754
  pilT Neisseria meningitidis 8013

68.116

100

0.681
  pilT Neisseria gonorrhoeae MS11

67.536

100

0.675
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.675

96.232

0.661
  pilT Vibrio cholerae strain A1552

68.675

96.232

0.661
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.522
  pilU Vibrio cholerae strain A1552

41.593

98.261

0.409
  pilU Pseudomonas stutzeri DSM 10701

41.994

95.942

0.403
  pilU Acinetobacter baylyi ADP1

38.728

100

0.388

Multiple sequence alignment