Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FSZ17_RS16930 Genome accession   NZ_CP042593
Coordinates   3533515..3534555 (-) Length   346 a.a.
NCBI ID   WP_057771772.1    Uniprot ID   A0A5B8Z6W2
Organism   Cytobacillus dafuensis strain FJAT-25496     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3528515..3539555
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FSZ17_RS16900 (FSZ17_16895) - 3528540..3529337 (-) 798 WP_057771760.1 hypothetical protein -
  FSZ17_RS16905 (FSZ17_16900) - 3529334..3529960 (-) 627 WP_057771762.1 hypothetical protein -
  FSZ17_RS16910 (FSZ17_16905) pilM 3529963..3530955 (-) 993 WP_057771764.1 type IV pilus biogenesis protein PilM -
  FSZ17_RS16915 (FSZ17_16910) comC 3530986..3531735 (-) 750 WP_057771766.1 A24 family peptidase Machinery gene
  FSZ17_RS16920 (FSZ17_16915) - 3531777..3532208 (-) 432 WP_057771768.1 type IV pilin protein -
  FSZ17_RS16925 (FSZ17_16920) - 3532308..3533513 (-) 1206 WP_057771770.1 type II secretion system F family protein -
  FSZ17_RS16930 (FSZ17_16925) pilT 3533515..3534555 (-) 1041 WP_057771772.1 type IV pilus twitching motility protein PilT Machinery gene
  FSZ17_RS16935 (FSZ17_16930) - 3534567..3536228 (-) 1662 WP_057771774.1 GspE/PulE family protein -
  FSZ17_RS16940 (FSZ17_16935) - 3536253..3537575 (-) 1323 WP_228460360.1 G5 domain-containing protein -
  FSZ17_RS16945 (FSZ17_16940) - 3537617..3538456 (-) 840 WP_057771778.1 hypothetical protein -
  FSZ17_RS16950 (FSZ17_16945) - 3538640..3539062 (+) 423 WP_057771780.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FSZ17_RS16955 (FSZ17_16950) - 3539068..3539538 (+) 471 WP_057771781.1 PilW family protein -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38623.76 Da        Isoelectric Point: 8.7716

>NTDB_id=378597 FSZ17_RS16930 WP_057771772.1 3533515..3534555(-) (pilT) [Cytobacillus dafuensis strain FJAT-25496]
MKDKIEYLLRAAFELKASDLHLTVGVQPIMRINGELKKYGKEILKPDETEGMAKAVIPDRMWTHFKDKGELDFSYGIPGV
SRFRVNTYHQRSCISLAIRVVPTKIPSIDELELPNVLRKISEKPQGLVLVTGPTGSGKSTTLASMIQYMNNTMRKHIITL
EDPIEYLHKHGNSIIDQREVGFDTMNFANGLRAALRQDPDIILVGEMRDLETIQTAITAAETGHLVLGTLHTSSAPATIN
RIIDVFPPSQQPQIRIQLASVLVAVISQRLFRREDKQGRKAATEILMNNAAVGNLIRNEKIHQIISIMQTSRAQGMHTLE
SSINDLVHAGIISKEAAEPFLQEIII

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=378597 FSZ17_RS16930 WP_057771772.1 3533515..3534555(-) (pilT) [Cytobacillus dafuensis strain FJAT-25496]
GTGAAAGATAAAATAGAATATTTATTACGCGCCGCATTTGAATTAAAAGCTTCAGATCTTCATTTAACGGTAGGCGTTCA
ACCTATTATGCGGATCAATGGCGAATTGAAAAAATATGGAAAAGAGATCCTGAAGCCCGATGAGACAGAAGGAATGGCAA
AAGCGGTTATACCGGATCGTATGTGGACTCATTTTAAGGATAAGGGAGAATTAGATTTTTCATATGGCATCCCAGGTGTT
TCTCGGTTTCGTGTAAATACATATCATCAACGTTCTTGTATTTCTCTGGCGATAAGGGTGGTTCCTACGAAAATTCCATC
CATTGATGAGTTGGAATTGCCCAATGTATTACGGAAGATTTCAGAAAAACCACAGGGCCTTGTCCTTGTGACAGGCCCAA
CTGGAAGCGGCAAATCAACAACCCTTGCTTCTATGATTCAGTACATGAATAATACGATGCGAAAACATATTATTACCCTT
GAAGATCCAATTGAGTATCTCCATAAACATGGGAATTCAATTATTGATCAGCGAGAAGTAGGATTTGATACGATGAATTT
TGCCAATGGCCTAAGAGCGGCATTACGGCAGGACCCTGACATAATCCTTGTCGGGGAAATGCGAGATTTGGAAACGATAC
AAACAGCGATTACGGCAGCTGAGACGGGGCATTTAGTGTTAGGAACCCTGCATACATCAAGTGCTCCAGCAACGATTAAT
CGAATAATTGATGTCTTTCCACCGTCCCAGCAGCCTCAAATTAGAATTCAACTGGCTTCTGTTTTAGTAGCGGTCATATC
CCAACGGCTTTTTCGCCGAGAAGATAAGCAAGGGCGGAAAGCAGCAACTGAAATATTAATGAATAATGCGGCGGTTGGCA
ATCTTATTCGCAATGAAAAGATCCATCAAATCATAAGCATTATGCAAACGTCTCGTGCTCAAGGAATGCATACACTTGAG
AGTAGTATTAATGATCTCGTCCATGCAGGCATTATTTCCAAAGAAGCTGCAGAACCCTTTTTACAGGAGATTATCATTTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5B8Z6W2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.755

95.665

0.486
  pilT Neisseria gonorrhoeae MS11

48.503

96.532

0.468
  pilT Neisseria meningitidis 8013

48.503

96.532

0.468
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.64

95.665

0.465
  pilT Vibrio cholerae strain A1552

48.64

95.665

0.465
  pilT Legionella pneumophila strain ERS1305867

47.147

96.243

0.454
  pilT Legionella pneumophila strain Lp02

47.147

96.243

0.454
  pilT Acinetobacter baylyi ADP1

47.72

95.087

0.454
  pilT Pseudomonas aeruginosa PAK

47.561

94.798

0.451
  pilT Pseudomonas stutzeri DSM 10701

47.416

95.087

0.451
  pilT Acinetobacter baumannii D1279779

47.256

94.798

0.448
  pilT Acinetobacter nosocomialis M2

47.256

94.798

0.448
  pilT Acinetobacter baumannii strain A118

47.256

94.798

0.448
  pilU Pseudomonas stutzeri DSM 10701

40.419

96.532

0.39
  pilU Acinetobacter baylyi ADP1

41.562

92.486

0.384
  pilU Vibrio cholerae strain A1552

40.373

93.064

0.376

Multiple sequence alignment