Detailed information NTG0376948

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: FLM31_RS09675
• Genome accession: NZ_CP042317
• Coordinates: 1999741..2000541 (-)
• Length: 266 a.a.
• NCBI ID: WP_004213441.1
• Uniprot ID: A0A9Q4U0T4
• Organism: Klebsiella pneumoniae strain 74-17
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1994741..2005541

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FLM31_RS09655	dksA	1995321..1995776 (-)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-
FLM31_RS09660	sfsA	1995939..1996655 (-)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
FLM31_RS09665	thpR	1996655..1997191 (-)	537	WP_004177389.1	RNA 2',3'-cyclic phosphodiesterase	-
FLM31_RS09670	hrpB	1997264..1999693 (+)	2430	WP_032434212.1	ATP-dependent helicase HrpB	-
FLM31_RS09675	pilD	1999741..2000541 (-)	801	WP_004213441.1	A24 family peptidase	Machinery gene
FLM31_RS09680	-	2000538..2001284 (-)	747	WP_032434210.1	type II secretion system protein N	-
FLM31_RS09685	-	2001274..2001759 (-)	486	WP_004145896.1	type II secretion system protein M	-
FLM31_RS09690	gspL	2001752..2002945 (-)	1194	WP_032434208.1	type II secretion system protein GspL	-
FLM31_RS09695	gspK	2002932..2003918 (-)	987	WP_077254116.1	type II secretion system minor pseudopilin GspK	-
FLM31_RS09700	gspJ	2003915..2004511 (-)	597	WP_032411156.1	type II secretion system minor pseudopilin GspJ	-
FLM31_RS09705	gspI	2004508..2004873 (-)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
FLM31_RS09710	gspH	2004870..2005385 (-)	516	WP_032414529.1	type II secretion system minor pseudopilin GspH	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29405.01 Da
• Isoelectric Point: 8.8894

>NTDB_id=376948 FLM31_RS09675 WP_004213441.1 1999741..2000541(-) (pilD) [Klebsiella pneumoniae strain 74-17]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPISWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=376948 FLM31_RS09675 WP_004213441.1 1999741..2000541(-) (pilD) [Klebsiella pneumoniae strain 74-17]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCTCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGAGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425