Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FFU37_RS12975 Genome accession   NZ_CP040558
Coordinates   2903258..2904298 (-) Length   346 a.a.
NCBI ID   WP_039488318.1    Uniprot ID   A0A7W2YGM4
Organism   Pseudoalteromonas distincta strain 16-SW-7     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2898258..2909298
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FFU37_RS12945 (FFU37_12945) - 2898567..2899067 (+) 501 WP_002961951.1 hypothetical protein -
  FFU37_RS12950 (FFU37_12950) rsmE 2899137..2899871 (+) 735 WP_002961950.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  FFU37_RS12955 (FFU37_12955) gshB 2899926..2900876 (+) 951 WP_138489656.1 glutathione synthase -
  FFU37_RS12960 (FFU37_12960) - 2900992..2901549 (+) 558 WP_002961948.1 YqgE/AlgH family protein -
  FFU37_RS12965 (FFU37_12965) ruvX 2901587..2902036 (+) 450 WP_138489657.1 Holliday junction resolvase RuvX -
  FFU37_RS12970 (FFU37_12970) pilU 2902112..2903248 (-) 1137 WP_024596219.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FFU37_RS12975 (FFU37_12975) pilT 2903258..2904298 (-) 1041 WP_039488318.1 type IV pilus twitching motility protein PilT Machinery gene
  FFU37_RS12980 (FFU37_12980) - 2904301..2905014 (+) 714 WP_002962566.1 YggS family pyridoxal phosphate-dependent enzyme -
  FFU37_RS12985 (FFU37_12985) proC 2905032..2905853 (+) 822 WP_039488316.1 pyrroline-5-carboxylate reductase -
  FFU37_RS12990 (FFU37_12990) - 2905880..2906413 (+) 534 WP_002962568.1 YggT family protein -
  FFU37_RS12995 (FFU37_12995) - 2906474..2906917 (+) 444 WP_138489658.1 DUF4426 domain-containing protein -
  FFU37_RS13000 (FFU37_13000) - 2906922..2907521 (+) 600 WP_002962573.1 XTP/dITP diphosphatase -
  FFU37_RS13005 (FFU37_13005) hemW 2907518..2908654 (+) 1137 WP_138489659.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38073.38 Da        Isoelectric Point: 6.2308

>NTDB_id=365203 FFU37_RS12975 WP_039488318.1 2903258..2904298(-) (pilT) [Pseudoalteromonas distincta strain 16-SW-7]
MDITELLAFSVQHKASDLHLSSGVSPMIRVDGDVRRINIPALADKDVNSLVYDIMNDNQRKDYEQNLEVDFSFEVPNLAR
FRVNAFNSNRGPAAVFRTIPSEVLTLDDLGAPDIFKTISDTPRGLVLVTGPTGSGKSTTLAAMVDYINQNKHHHILTIED
PIEFVHDNKLSLINQREVHRDTHSFSNALRSALREDPDVILVGELRDLETIRLAMTAAETGHLVFGTLHTTSAPKTIDRI
IDVFPGEEKAMVRSMLSESLRAVISQTLLKKIGGGRVAAHEIMIAVPAIRNLIREDKIAQMYSSIQTGASVGMQTMDQCL
TNLVNHGIVTNAAAKEKAQDKTQFGG

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=365203 FFU37_RS12975 WP_039488318.1 2903258..2904298(-) (pilT) [Pseudoalteromonas distincta strain 16-SW-7]
ATGGATATTACCGAATTATTAGCGTTTAGTGTGCAACACAAAGCATCCGATTTACATTTATCGTCGGGTGTTTCACCTAT
GATACGCGTAGACGGTGATGTTCGCCGCATTAATATACCAGCCCTTGCAGACAAAGACGTAAACAGTCTGGTTTACGATA
TTATGAACGATAATCAACGCAAGGACTACGAACAAAATCTCGAAGTGGATTTTTCGTTTGAAGTGCCTAATTTAGCACGT
TTTCGTGTGAATGCATTTAACTCTAATCGCGGGCCTGCTGCGGTATTTCGTACCATACCAAGTGAAGTGTTAACGCTTGA
TGATTTAGGCGCACCAGATATTTTTAAAACAATCTCTGATACCCCACGTGGACTCGTGTTAGTAACAGGGCCAACGGGTT
CTGGTAAATCAACAACACTTGCCGCTATGGTCGACTATATAAATCAAAATAAACATCACCATATTCTAACAATAGAAGAT
CCAATCGAGTTTGTTCACGATAATAAATTAAGTTTAATTAACCAACGCGAAGTTCACCGCGATACACATAGCTTTTCAAA
CGCGCTACGTAGTGCATTGCGTGAAGACCCTGATGTAATACTTGTGGGTGAGTTACGTGATTTAGAAACAATTCGTCTTG
CAATGACCGCTGCCGAAACGGGTCACTTAGTGTTTGGCACGCTACATACAACATCTGCACCAAAAACTATTGACCGTATT
ATTGATGTATTCCCAGGTGAAGAAAAAGCTATGGTGCGCTCAATGTTATCTGAATCTTTACGTGCGGTAATTTCACAAAC
ACTGCTTAAAAAAATTGGTGGTGGGCGAGTTGCAGCACACGAAATTATGATTGCAGTTCCTGCTATTCGTAACTTAATTC
GTGAAGATAAAATTGCACAAATGTATTCGTCTATTCAAACCGGTGCGTCTGTGGGCATGCAAACAATGGATCAGTGTTTA
ACTAATTTAGTTAATCACGGTATTGTTACAAATGCGGCAGCGAAAGAAAAAGCGCAAGACAAAACACAATTTGGTGGGTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7W2YGM4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

76.453

99.422

0.76
  pilT Acinetobacter nosocomialis M2

76.163

99.422

0.757
  pilT Acinetobacter baumannii D1279779

76.163

99.422

0.757
  pilT Acinetobacter baumannii strain A118

76.163

99.422

0.757
  pilT Pseudomonas aeruginosa PAK

75.872

99.422

0.754
  pilT Acinetobacter baylyi ADP1

75.291

99.422

0.749
  pilT Legionella pneumophila strain Lp02

72.674

99.422

0.723
  pilT Legionella pneumophila strain ERS1305867

72.674

99.422

0.723
  pilT Neisseria gonorrhoeae MS11

70.381

98.555

0.694
  pilT Neisseria meningitidis 8013

70.381

98.555

0.694
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.796

97.977

0.694
  pilT Vibrio cholerae strain A1552

70.796

97.977

0.694
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

99.711

0.52
  pilU Vibrio cholerae strain A1552

42.462

93.931

0.399
  pilU Acinetobacter baylyi ADP1

39.118

98.266

0.384
  pilU Pseudomonas stutzeri DSM 10701

40.181

95.665

0.384

Multiple sequence alignment