Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   FFU37_RS12970 Genome accession   NZ_CP040558
Coordinates   2902112..2903248 (-) Length   378 a.a.
NCBI ID   WP_024596219.1    Uniprot ID   A0A7W2YG75
Organism   Pseudoalteromonas distincta strain 16-SW-7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2897112..2908248
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FFU37_RS12940 (FFU37_12940) - 2897609..2898502 (+) 894 WP_039488320.1 hypothetical protein -
  FFU37_RS12945 (FFU37_12945) - 2898567..2899067 (+) 501 WP_002961951.1 hypothetical protein -
  FFU37_RS12950 (FFU37_12950) rsmE 2899137..2899871 (+) 735 WP_002961950.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  FFU37_RS12955 (FFU37_12955) gshB 2899926..2900876 (+) 951 WP_138489656.1 glutathione synthase -
  FFU37_RS12960 (FFU37_12960) - 2900992..2901549 (+) 558 WP_002961948.1 YqgE/AlgH family protein -
  FFU37_RS12965 (FFU37_12965) ruvX 2901587..2902036 (+) 450 WP_138489657.1 Holliday junction resolvase RuvX -
  FFU37_RS12970 (FFU37_12970) pilU 2902112..2903248 (-) 1137 WP_024596219.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FFU37_RS12975 (FFU37_12975) pilT 2903258..2904298 (-) 1041 WP_039488318.1 type IV pilus twitching motility protein PilT Machinery gene
  FFU37_RS12980 (FFU37_12980) - 2904301..2905014 (+) 714 WP_002962566.1 YggS family pyridoxal phosphate-dependent enzyme -
  FFU37_RS12985 (FFU37_12985) proC 2905032..2905853 (+) 822 WP_039488316.1 pyrroline-5-carboxylate reductase -
  FFU37_RS12990 (FFU37_12990) - 2905880..2906413 (+) 534 WP_002962568.1 YggT family protein -
  FFU37_RS12995 (FFU37_12995) - 2906474..2906917 (+) 444 WP_138489658.1 DUF4426 domain-containing protein -
  FFU37_RS13000 (FFU37_13000) - 2906922..2907521 (+) 600 WP_002962573.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41944.81 Da        Isoelectric Point: 5.4587

>NTDB_id=365202 FFU37_RS12970 WP_024596219.1 2902112..2903248(-) (pilU) [Pseudoalteromonas distincta strain 16-SW-7]
MALSLNDFLLIMIEKKGSDLFVSSQLPVSAKINGELTALSDDRLTDEQSLELVESAMSEKQKAEFHTTKECNFAIATDEG
RFRISAFWQRDRAGMVIRRIVTQIPDVSDLGLPSTLTDVIMSKRGLVLFVGGTGTGKSTSLAALIGYRNRNQRGHILTIE
DPIEFVHEHRKSIITQREVGLDTESFESALKSSLRQAPDVILIGEIRSQETMEYALSFAETGHLCVATLHANNANQAIDR
IMHLVPKEKHDKLKYDLALNLRAIVAQQLVPTADGESRVAAIEILLNSPMVAELIKNGDIGGIKEAMAKSKEMGMQTFDQ
ALFELYRQQRINYADALHHADSPNDLRLMIKLRNNEQQGAGFLQGVTIDGLDDKNSDQ

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=365202 FFU37_RS12970 WP_024596219.1 2902112..2903248(-) (pilU) [Pseudoalteromonas distincta strain 16-SW-7]
ATGGCTTTATCTTTAAACGACTTTTTACTCATAATGATTGAGAAAAAGGGTTCTGACCTATTTGTATCGAGTCAGTTACC
TGTAAGTGCAAAAATAAACGGTGAACTAACAGCTCTCAGTGACGATAGACTGACTGATGAGCAATCGCTTGAGTTAGTAG
AATCTGCAATGAGCGAAAAGCAAAAAGCAGAGTTTCATACAACGAAAGAATGTAACTTTGCGATTGCTACCGACGAAGGG
CGTTTTCGTATTTCTGCTTTTTGGCAGCGCGATAGAGCAGGTATGGTTATTCGCCGTATTGTGACGCAAATTCCTGATGT
GAGTGATTTAGGTCTGCCGTCTACACTGACAGATGTAATTATGTCTAAACGCGGTCTTGTCTTATTTGTAGGTGGTACAG
GTACGGGTAAGTCGACCTCACTAGCTGCACTTATTGGTTATCGAAACCGTAATCAACGAGGTCATATACTGACCATTGAA
GATCCGATTGAATTTGTTCATGAGCATCGTAAAAGTATTATTACTCAGCGCGAAGTAGGGCTTGATACAGAAAGCTTTGA
GTCGGCACTTAAAAGCTCATTACGCCAAGCTCCAGATGTTATTTTGATTGGTGAAATTCGATCGCAAGAAACCATGGAAT
ACGCCCTAAGCTTTGCTGAAACCGGTCACCTATGTGTTGCGACACTACATGCTAATAATGCGAACCAAGCGATTGACCGT
ATTATGCATTTAGTACCAAAAGAAAAACACGATAAGCTTAAGTACGACTTAGCACTTAACTTACGCGCCATTGTTGCTCA
GCAATTAGTACCGACTGCTGATGGTGAAAGCCGAGTTGCAGCTATTGAAATATTGCTTAACTCACCAATGGTCGCTGAAC
TGATTAAAAATGGTGATATTGGCGGAATTAAAGAGGCAATGGCTAAGTCGAAAGAAATGGGCATGCAAACCTTTGACCAA
GCGTTATTTGAGTTGTACAGACAGCAGCGAATTAACTACGCTGATGCACTGCACCATGCTGACTCACCAAATGATTTACG
CTTAATGATAAAACTGCGCAATAACGAACAACAAGGTGCGGGCTTTTTACAGGGCGTTACTATTGATGGCCTTGATGATA
AAAATAGCGATCAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(28-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7W2YG75

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

57.067

99.206

0.566
  pilU Acinetobacter baylyi ADP1

56.499

99.735

0.563
  pilU Vibrio cholerae strain A1552

55.586

97.09

0.54
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.659

95.503

0.407

Multiple sequence alignment