Detailed information NTG0035418

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: ADEG_RS08705
• Genome accession: NC_013385
• Coordinates: 1714453..1715535 (-)
• Length: 360 a.a.
• NCBI ID: WP_015739693.1
• Uniprot ID: C9R937
• Organism: Ammonifex degensii KC4
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1709453..1720535

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ADEG_RS08680 (Adeg_1719)	-	1710029..1711519 (-)	1491	WP_015739688.1	pilus assembly PilX N-terminal domain-containing protein	-
ADEG_RS08685 (Adeg_1720)	-	1711516..1711983 (-)	468	WP_015739689.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
ADEG_RS08690 (Adeg_1721)	-	1711980..1712546 (-)	567	WP_015739690.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
ADEG_RS08695 (Adeg_1722)	-	1712662..1713093 (-)	432	WP_015739691.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
ADEG_RS08700 (Adeg_1723)	-	1713217..1714446 (-)	1230	WP_015739692.1	type II secretion system F family protein	-
ADEG_RS08705 (Adeg_1724)	pilT	1714453..1715535 (-)	1083	WP_015739693.1	type IV pilus twitching motility protein PilT	Machinery gene
ADEG_RS08710 (Adeg_1725)	gspE	1715543..1717204 (-)	1662	WP_015739694.1	type II secretion system ATPase GspE	-
ADEG_RS08730 (Adeg_1726)	rpoD	1717804..1718913 (-)	1110	WP_015739695.1	RNA polymerase sigma factor RpoD	-

Sequence

Protein

• Length: 360 a.a.
• Molecular weight: 39695.99 Da
• Isoelectric Point: 8.5077

>NTDB_id=35418 ADEG_RS08705 WP_015739693.1 1714453..1715535(-) (pilT) [Ammonifex degensii KC4]
MRMADLVALAVRLGASDIHLTVGKPPVFRINGVLYPLDDPALSQEIFARPLAPEDTLRLAQEVMTPQQYARFEQEGEIDF
SFGFPGVARVRANIFKQRGSVALALRILSSRIPTFQELGLPDVVAYLARRPAGLVLVTGPTGSGKSTTLAAMINLINREK
RLHIITLEDPIEYLHRHEKSIVNQREVGQDTKSFARALRAALREDPDVIMVGEMRDLETIATAITAAETGHLVLATLHTV
SAAETVDRIVDVFPPSQQQQIRVQLANVIEGVISQLLLPRADGKGRVLALEIMVATPAIRNLIREGKTHQITSHIQTGGR
WGMQTMDAALASLYRQGLISREELLNRARDLELLQRLVGV

Nucleotide

• Length: 1083 bp

>NTDB_id=35418 ADEG_RS08705 WP_015739693.1 1714453..1715535(-) (pilT) [Ammonifex degensii KC4]
ATGCGCATGGCCGATCTGGTGGCGCTGGCCGTGAGGCTGGGGGCTTCCGACATTCACCTGACTGTCGGGAAGCCGCCCGT
TTTCCGTATAAACGGGGTGCTTTATCCCCTTGACGACCCGGCGCTTTCACAAGAAATCTTTGCCCGCCCCCTTGCGCCGG
AGGACACCCTACGGCTGGCCCAGGAGGTCATGACCCCCCAGCAGTACGCCCGCTTCGAGCAGGAGGGGGAGATCGACTTT
TCCTTCGGCTTTCCCGGGGTGGCGCGGGTACGGGCCAACATCTTCAAGCAGCGGGGGAGCGTGGCTTTAGCTCTGCGCAT
CTTGAGCTCCCGCATCCCCACCTTTCAGGAGTTGGGTCTGCCCGATGTGGTGGCCTACCTGGCGCGGCGCCCGGCGGGCT
TAGTGCTGGTGACCGGCCCCACCGGTTCGGGCAAGTCCACTACTCTTGCCGCCATGATAAACCTCATCAACCGGGAGAAG
CGGCTGCACATCATCACCCTGGAGGATCCCATCGAGTACCTGCACCGGCACGAGAAGAGCATCGTCAACCAGCGGGAGGT
GGGGCAGGACACCAAGTCTTTTGCTCGGGCCCTGCGGGCGGCTTTGCGGGAGGATCCGGACGTCATCATGGTGGGGGAGA
TGCGGGACCTGGAGACCATCGCCACGGCCATAACGGCGGCGGAGACGGGGCATTTGGTGCTGGCCACGCTACACACCGTC
TCGGCGGCGGAGACGGTGGACCGGATCGTGGACGTCTTCCCTCCCAGCCAGCAGCAACAGATAAGGGTGCAGCTGGCCAA
CGTGATCGAAGGGGTGATCTCGCAGCTTTTGCTGCCCCGCGCCGACGGGAAGGGAAGGGTGCTGGCCCTGGAGATCATGG
TGGCTACTCCCGCCATCCGCAACCTCATCCGGGAAGGGAAGACCCATCAGATAACTTCCCACATCCAGACCGGCGGGCGG
TGGGGGATGCAGACCATGGATGCGGCCTTAGCCTCCCTTTATCGCCAGGGGCTCATCTCCCGAGAGGAGCTCTTGAACCG
GGCCCGCGACCTGGAGCTACTCCAGAGACTGGTGGGGGTGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (12-269)

3D structure

• AlphaFold DB: C9R937

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	56.901	98.611	0.561
pilT	Neisseria meningitidis 8013	50.852	97.778	0.497
pilT	Pseudomonas aeruginosa PAK	51.003	96.944	0.494
pilT	Pseudomonas stutzeri DSM 10701	50.425	98.056	0.494
pilT	Neisseria gonorrhoeae MS11	50.568	97.778	0.494
pilT	Vibrio cholerae strain A1552	49.568	96.389	0.478
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	49.568	96.389	0.478
pilT	Acinetobacter baumannii D1279779	49.706	94.444	0.469
pilT	Acinetobacter nosocomialis M2	49.706	94.444	0.469
pilT	Acinetobacter baumannii strain A118	49.706	94.444	0.469
pilT	Legionella pneumophila strain Lp02	48.011	97.778	0.469
pilT	Legionella pneumophila strain ERS1305867	48.011	97.778	0.469
pilT	Acinetobacter baylyi ADP1	49.118	94.444	0.464
pilU	Pseudomonas stutzeri DSM 10701	41.389	100	0.414
pilU	Vibrio cholerae strain A1552	40.525	95.278	0.386
pilB/pilB1	Synechocystis sp. PCC 6803	32.84	100	0.369
pilU	Acinetobacter baylyi ADP1	38.109	96.944	0.369