Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   C9E88_RS04090 Genome accession   NZ_CP035934
Coordinates   870936..872057 (-) Length   373 a.a.
NCBI ID   WP_106985502.1    Uniprot ID   A0A7Y8Q3Y9
Organism   Acinetobacter cumulans strain WCHAc060092     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 865936..877057
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C9E88_RS04060 (C9E88_000190) - 866702..867550 (+) 849 WP_106985522.1 class II glutamine amidotransferase -
  C9E88_RS04065 (C9E88_000185) - 867827..868282 (+) 456 WP_106985498.1 bacteriohemerythrin -
  C9E88_RS04070 (C9E88_000180) - 868424..869506 (+) 1083 WP_106985499.1 hypothetical protein -
  C9E88_RS04075 (C9E88_000175) - 869506..869829 (+) 324 WP_106985500.1 RnfH family protein -
  C9E88_RS04080 (C9E88_000170) bamE 869873..870271 (-) 399 WP_106985501.1 outer membrane protein assembly factor BamE -
  C9E88_RS04085 (C9E88_000165) fur 870384..870821 (+) 438 WP_067766225.1 ferric iron uptake transcriptional regulator -
  C9E88_RS04090 (C9E88_000160) pilU 870936..872057 (-) 1122 WP_106985502.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  C9E88_RS04095 (C9E88_000155) pilT 872089..873126 (-) 1038 WP_106985503.1 type IV pilus twitching motility protein PilT Machinery gene
  C9E88_RS04100 (C9E88_000150) - 873250..873939 (+) 690 WP_106985504.1 YggS family pyridoxal phosphate-dependent enzyme -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41722.94 Da        Isoelectric Point: 6.5944

>NTDB_id=345154 C9E88_RS04090 WP_106985502.1 870936..872057(-) (pilU) [Acinetobacter cumulans strain WCHAc060092]
MDFNGLLDFMVEKKASDLFITADVEPSMKINGQIVPIGSTKLPGSTVGQLLNAIMNDKQRKEFAETRECNFAISNPSKSA
RFRVSAFQQRDEPGMVLRRIETIIPTMEQLKLPPILKELAMTKRGIIIFVGATGTGKSTSLASLVGYRNQNSKGHIITIE
DPIEFVHEHQGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVFATLHANNANQALDR
IIHFFEADRHNQLFMDLSLNLKAVVAQQLIPTIDGNSRRAAIEILINSPLISDMIRKGDVHEIKDLMKRSRELGMQTFDQ
ALFDLYKAKQISYKDALKHADSPNDLRLQIKLSEEGGDRLMNASTNITFDGQV

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=345154 C9E88_RS04090 WP_106985502.1 870936..872057(-) (pilU) [Acinetobacter cumulans strain WCHAc060092]
ATGGATTTTAACGGTTTGCTCGACTTTATGGTGGAAAAAAAGGCCTCTGACCTTTTTATTACTGCCGATGTCGAGCCGTC
CATGAAAATTAATGGTCAAATTGTACCTATTGGCTCTACAAAACTGCCAGGTTCAACGGTTGGCCAATTATTAAATGCCA
TCATGAACGATAAACAGCGTAAAGAGTTTGCAGAAACACGTGAATGTAACTTTGCAATCAGCAATCCATCTAAATCAGCG
CGTTTCCGTGTGAGTGCATTTCAGCAACGTGATGAGCCAGGCATGGTGCTCCGTCGTATTGAAACGATCATTCCGACTAT
GGAGCAACTCAAGTTACCGCCAATTTTAAAAGAATTAGCGATGACCAAGCGCGGTATTATCATCTTTGTGGGTGCAACAG
GTACAGGTAAATCTACATCCCTAGCATCACTCGTGGGTTATCGTAACCAAAACTCAAAAGGTCATATCATCACCATTGAA
GACCCGATTGAATTTGTACACGAGCATCAAGGTTGCATTATTACCCAGCGTGAAGTGGGTATTGATACCGATTCTTTTGA
AATCGCACTGAAAAATACCTTACGTCAGGCACCTGATGTCATTTTGATTGGTGAGATTCGTTCACGTGAAACAATGGACT
ATGCCATTGCCTTTGCTGAAACAGGTCACTTGGTATTTGCGACACTACATGCCAACAACGCCAACCAAGCATTAGATCGT
ATCATTCACTTCTTTGAAGCAGATCGTCACAACCAATTGTTTATGGATTTATCGTTAAATCTAAAAGCCGTGGTTGCACA
ACAGTTGATTCCAACCATTGATGGTAACTCTCGCCGTGCCGCGATTGAAATTTTGATCAACTCACCTTTAATTTCAGACA
TGATCCGTAAAGGTGATGTACATGAAATTAAAGACCTGATGAAACGTTCTCGTGAATTAGGCATGCAAACCTTTGACCAA
GCCCTGTTTGACCTTTATAAAGCCAAGCAAATTAGCTACAAAGATGCACTCAAACACGCCGATTCACCAAACGACTTACG
CCTACAAATCAAATTGTCTGAAGAAGGTGGAGATCGCTTAATGAATGCCAGCACCAACATTACCTTCGATGGTCAAGTGT
AG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Y8Q3Y9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

74.603

100

0.756
  pilU Pseudomonas stutzeri DSM 10701

68.464

99.464

0.681
  pilU Vibrio cholerae strain A1552

56.216

99.196

0.558
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.311

94.102

0.389
  pilT Vibrio cholerae strain A1552

41.311

94.102

0.389
  pilT Acinetobacter nosocomialis M2

41.983

91.957

0.386
  pilT Acinetobacter baumannii D1279779

41.983

91.957

0.386
  pilT Acinetobacter baumannii strain A118

41.983

91.957

0.386
  pilT Legionella pneumophila strain Lp02

42.857

90.08

0.386
  pilT Legionella pneumophila strain ERS1305867

42.857

90.08

0.386
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.618

92.761

0.386
  pilT Pseudomonas aeruginosa PAK

41.329

92.761

0.383
  pilT Acinetobacter baylyi ADP1

41.399

91.957

0.381
  pilT Pseudomonas stutzeri DSM 10701

40.173

92.761

0.373
  pilT Neisseria meningitidis 8013

39.306

92.761

0.365
  pilT Neisseria gonorrhoeae MS11

39.306

92.761

0.365

Multiple sequence alignment