Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   C9E88_RS04095 Genome accession   NZ_CP035934
Coordinates   872089..873126 (-) Length   345 a.a.
NCBI ID   WP_106985503.1    Uniprot ID   A0A7Y8Q403
Organism   Acinetobacter cumulans strain WCHAc060092     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 867089..878126
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C9E88_RS04065 (C9E88_000185) - 867827..868282 (+) 456 WP_106985498.1 bacteriohemerythrin -
  C9E88_RS04070 (C9E88_000180) - 868424..869506 (+) 1083 WP_106985499.1 hypothetical protein -
  C9E88_RS04075 (C9E88_000175) - 869506..869829 (+) 324 WP_106985500.1 RnfH family protein -
  C9E88_RS04080 (C9E88_000170) bamE 869873..870271 (-) 399 WP_106985501.1 outer membrane protein assembly factor BamE -
  C9E88_RS04085 (C9E88_000165) fur 870384..870821 (+) 438 WP_067766225.1 ferric iron uptake transcriptional regulator -
  C9E88_RS04090 (C9E88_000160) pilU 870936..872057 (-) 1122 WP_106985502.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  C9E88_RS04095 (C9E88_000155) pilT 872089..873126 (-) 1038 WP_106985503.1 type IV pilus twitching motility protein PilT Machinery gene
  C9E88_RS04100 (C9E88_000150) - 873250..873939 (+) 690 WP_106985504.1 YggS family pyridoxal phosphate-dependent enzyme -
  C9E88_RS04105 (C9E88_000145) - 873997..877593 (-) 3597 WP_106985505.1 AAA family ATPase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38303.96 Da        Isoelectric Point: 6.6657

>NTDB_id=345155 C9E88_RS04095 WP_106985503.1 872089..873126(-) (pilT) [Acinetobacter cumulans strain WCHAc060092]
MDITELLAFSAKNGASDLHLSAGMPPLIRVDGEVRRINLPALEHKEVHKLVYDIMNDKQRRDYEEKLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMEDLGMGQIFKEICEYPRGIVLVTGPTGSGKSTTLAAMMDYINDNRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANYGMTTLDQSL
KTLVSKGIISPQTARTAAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=345155 C9E88_RS04095 WP_106985503.1 872089..873126(-) (pilT) [Acinetobacter cumulans strain WCHAc060092]
ATGGATATCACAGAATTATTGGCATTTTCGGCTAAAAATGGCGCTTCAGATTTACACTTATCAGCAGGTATGCCTCCACT
GATCCGTGTAGATGGTGAAGTTCGTCGTATTAACTTGCCTGCATTAGAACATAAAGAAGTACACAAATTGGTGTACGACA
TTATGAATGACAAGCAACGCCGTGATTATGAAGAAAAATTGGAAACAGACTTTTCATTTGAAGTTCCAGGCGTGGCACGT
TTCCGTGTGAACGCATTTAACCAAAACCGCGGTGCTGGTGCGGTATTCCGTACCATTCCATCAAAAGTGCTTTCAATGGA
AGATTTGGGCATGGGGCAAATCTTTAAAGAGATTTGTGAATACCCACGCGGTATCGTACTGGTGACTGGGCCTACAGGTT
CGGGTAAATCGACGACATTGGCAGCCATGATGGACTACATCAATGACAACCGTTATGACCATATTTTGACGGTCGAAGAT
CCAATCGAATTTGTACACCAATCAAAAAAATGCCTAATTAACCAGCGTGAAGTCCACCGCGATACCCACGGCTTTAACGA
AGCACTTCGTTCAGCACTGCGTGAAGACCCTGACATTATCTTGGTCGGTGAGATGCGTGACCTTGAAACGATTCGTTTGG
CGCTTACAGCTGCTGAAACTGGTCACTTAGTATTTGGTACGCTACATACCACATCTGCGGCTAAAACCATTGACCGTGTG
ATTGACGTATTCCCTGCTGAAGAAAAAGATATGGTTCGTGCCATGTTGTCAGAATCCCTACAAGCGGTTATTTCACAAAC
ACTATTAAAGAAAAATGGTGGTGGCCGTGTGGCAGCGCATGAAATCATGATCGGTATTCCTGCAATCCGTAACTTGATTC
GTGAAAACAAAGTCGCGCAAATGTACTCTGCCATTCAAACGGGTGCAAACTACGGCATGACAACGCTAGACCAAAGCTTA
AAAACTTTAGTGTCTAAAGGCATCATTAGCCCACAAACTGCACGTACTGCAGCAAAACAGCCAGAATCTTTCTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Y8Q403

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

95.652

100

0.957
  pilT Acinetobacter baumannii D1279779

95.362

100

0.954
  pilT Acinetobacter baumannii strain A118

95.362

100

0.954
  pilT Acinetobacter baylyi ADP1

86.919

99.71

0.867
  pilT Pseudomonas aeruginosa PAK

83.721

99.71

0.835
  pilT Pseudomonas stutzeri DSM 10701

83.43

99.71

0.832
  pilT Legionella pneumophila strain Lp02

75.581

99.71

0.754
  pilT Legionella pneumophila strain ERS1305867

75.581

99.71

0.754
  pilT Neisseria meningitidis 8013

68.406

100

0.684
  pilT Neisseria gonorrhoeae MS11

68.116

100

0.681
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.75

97.391

0.67
  pilT Vibrio cholerae strain A1552

68.75

97.391

0.67
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

100

0.51
  pilU Vibrio cholerae strain A1552

41.003

98.261

0.403
  pilU Acinetobacter baylyi ADP1

39.306

100

0.394
  pilU Pseudomonas stutzeri DSM 10701

40

97.101

0.388

Multiple sequence alignment