Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   EJN92_RS13365 Genome accession   NZ_CP034464
Coordinates   3058465..3059508 (-) Length   347 a.a.
NCBI ID   WP_126128287.1    Uniprot ID   A0A3Q9BRL2
Organism   Undibacterium parvum strain DSM 23061     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3053465..3064508
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EJN92_RS13340 (EJN92_13340) - 3053755..3054675 (+) 921 WP_126128282.1 DMT family transporter -
  EJN92_RS13345 (EJN92_13345) - 3054837..3055688 (+) 852 WP_126128283.1 sulfurtransferase -
  EJN92_RS13350 (EJN92_13350) - 3055734..3056435 (-) 702 WP_407701558.1 ZIP family metal transporter -
  EJN92_RS22010 - 3056371..3056505 (+) 135 WP_265415630.1 hypothetical protein -
  EJN92_RS13355 (EJN92_13355) - 3056755..3057237 (-) 483 WP_126128285.1 glutathione peroxidase -
  EJN92_RS13360 (EJN92_13360) pilU 3057234..3058370 (-) 1137 WP_126128286.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EJN92_RS13365 (EJN92_13365) pilT 3058465..3059508 (-) 1044 WP_126128287.1 type IV pilus twitching motility protein PilT Machinery gene
  EJN92_RS13370 (EJN92_13370) - 3059556..3060260 (+) 705 WP_126128288.1 YggS family pyridoxal phosphate-dependent enzyme -
  EJN92_RS13375 (EJN92_13375) proC 3060250..3061077 (+) 828 WP_126128289.1 pyrroline-5-carboxylate reductase -
  EJN92_RS13380 (EJN92_13380) - 3061144..3061464 (-) 321 WP_126128290.1 YqjK family protein -
  EJN92_RS13385 (EJN92_13385) - 3061468..3061845 (-) 378 WP_126128291.1 phage holin family protein -
  EJN92_RS13390 (EJN92_13390) - 3061849..3062157 (-) 309 WP_126128292.1 YqjD family protein -
  EJN92_RS13395 (EJN92_13395) ubiA 3062303..3063169 (-) 867 WP_126128293.1 4-hydroxybenzoate octaprenyltransferase -
  EJN92_RS13400 (EJN92_13400) - 3063286..3064230 (-) 945 WP_126128294.1 LysR substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38111.63 Da        Isoelectric Point: 6.8708

>NTDB_id=331491 EJN92_RS13365 WP_126128287.1 3058465..3059508(-) (pilT) [Undibacterium parvum strain DSM 23061]
MDISELLAFSVKNNASDLHLSSGLPPMIRVHGDVRRINLPALEHKDVHGLIYDIMNDSQRKHYEENLECDFSFSIPGLAR
FRVNAFNQERGAAAVMRTIPSKILTLEQLNAPKIFADFALKARGLVLVTGPTGSGKSTTLAGMVNHINEHEYGHILTVED
PIEFVHESKKCLINQREVGPHTHSFNNALRSALREDPDVILVGELRDLETIRLALTAAETGHMVFGTLHTSSAAKTIDRI
VDVFPAEEKEMVRSMLSESLQAVISQSLLKTKDGSGRVAVHEIMVGTPAIRNLIREAKIAQMYSAIQTGSNVGMQTLDQN
LTDLVRRNVISASAARAAAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=331491 EJN92_RS13365 WP_126128287.1 3058465..3059508(-) (pilT) [Undibacterium parvum strain DSM 23061]
ATGGACATTTCAGAATTACTGGCATTTTCGGTCAAAAATAACGCCTCCGATCTGCACCTATCTTCAGGTCTTCCCCCCAT
GATACGTGTACATGGCGATGTCCGGCGCATCAATTTGCCCGCATTGGAACATAAAGATGTACATGGTCTGATTTATGACA
TCATGAACGATTCCCAGCGCAAGCACTATGAAGAGAATCTGGAATGCGATTTTTCTTTTTCAATTCCTGGTTTGGCGCGT
TTCCGTGTCAATGCATTTAATCAGGAACGTGGTGCAGCAGCAGTAATGCGGACCATTCCATCAAAAATTCTGACGCTAGA
ACAATTAAATGCACCAAAAATTTTTGCTGATTTCGCGCTCAAAGCGCGTGGCTTGGTTTTGGTAACCGGGCCTACCGGTT
CGGGTAAATCAACCACCCTCGCTGGCATGGTCAACCACATCAATGAACATGAGTACGGGCATATTTTGACGGTGGAAGAT
CCGATTGAATTCGTCCATGAATCAAAAAAATGCCTGATTAATCAGCGCGAAGTCGGGCCACACACGCATTCGTTTAACAA
CGCCTTGCGCTCGGCCTTGCGCGAAGATCCGGATGTGATTTTGGTCGGTGAGTTACGCGATCTGGAAACCATACGTCTGG
CCCTAACCGCAGCAGAAACCGGCCACATGGTGTTCGGCACACTGCATACCTCGTCGGCCGCCAAAACCATAGACAGGATA
GTCGACGTCTTTCCGGCAGAAGAAAAAGAAATGGTGCGCTCCATGCTTTCTGAATCTCTGCAAGCGGTAATCTCGCAGTC
ACTGCTCAAAACCAAAGATGGCTCGGGTCGCGTTGCCGTGCATGAGATCATGGTTGGCACACCAGCGATACGCAATCTGA
TACGCGAAGCAAAAATCGCCCAGATGTATTCTGCCATCCAGACCGGTAGCAATGTGGGCATGCAAACTCTGGATCAAAAT
TTAACTGACTTGGTGAGACGCAATGTGATCTCCGCCAGTGCCGCGCGCGCTGCCGCCAAGATCCCAGAGAATTTCCCGGG
CTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3Q9BRL2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.391

99.424

0.769
  pilT Acinetobacter baumannii D1279779

77.391

99.424

0.769
  pilT Acinetobacter baumannii strain A118

77.391

99.424

0.769
  pilT Pseudomonas aeruginosa PAK

75.362

99.424

0.749
  pilT Acinetobacter baylyi ADP1

75.072

99.424

0.746
  pilT Pseudomonas stutzeri DSM 10701

74.783

99.424

0.744
  pilT Legionella pneumophila strain Lp02

74.631

97.695

0.729
  pilT Legionella pneumophila strain ERS1305867

74.631

97.695

0.729
  pilT Neisseria gonorrhoeae MS11

68.696

99.424

0.683
  pilT Neisseria meningitidis 8013

68.696

99.424

0.683
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.326

97.118

0.683
  pilT Vibrio cholerae strain A1552

70.326

97.118

0.683
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

99.424

0.507
  pilU Vibrio cholerae strain A1552

42.388

96.542

0.409
  pilU Pseudomonas stutzeri DSM 10701

41.593

97.695

0.406
  pilU Acinetobacter baylyi ADP1

40.751

99.712

0.406

Multiple sequence alignment