Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   EJN92_RS13360 Genome accession   NZ_CP034464
Coordinates   3057234..3058370 (-) Length   378 a.a.
NCBI ID   WP_126128286.1    Uniprot ID   A0A3Q9BRM5
Organism   Undibacterium parvum strain DSM 23061     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3052234..3063370
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EJN92_RS13335 (EJN92_13335) - 3052594..3053709 (+) 1116 WP_126128281.1 SRPBCC family protein -
  EJN92_RS13340 (EJN92_13340) - 3053755..3054675 (+) 921 WP_126128282.1 DMT family transporter -
  EJN92_RS13345 (EJN92_13345) - 3054837..3055688 (+) 852 WP_126128283.1 sulfurtransferase -
  EJN92_RS13350 (EJN92_13350) - 3055734..3056435 (-) 702 WP_407701558.1 ZIP family metal transporter -
  EJN92_RS22010 - 3056371..3056505 (+) 135 WP_265415630.1 hypothetical protein -
  EJN92_RS13355 (EJN92_13355) - 3056755..3057237 (-) 483 WP_126128285.1 glutathione peroxidase -
  EJN92_RS13360 (EJN92_13360) pilU 3057234..3058370 (-) 1137 WP_126128286.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EJN92_RS13365 (EJN92_13365) pilT 3058465..3059508 (-) 1044 WP_126128287.1 type IV pilus twitching motility protein PilT Machinery gene
  EJN92_RS13370 (EJN92_13370) - 3059556..3060260 (+) 705 WP_126128288.1 YggS family pyridoxal phosphate-dependent enzyme -
  EJN92_RS13375 (EJN92_13375) proC 3060250..3061077 (+) 828 WP_126128289.1 pyrroline-5-carboxylate reductase -
  EJN92_RS13380 (EJN92_13380) - 3061144..3061464 (-) 321 WP_126128290.1 YqjK family protein -
  EJN92_RS13385 (EJN92_13385) - 3061468..3061845 (-) 378 WP_126128291.1 phage holin family protein -
  EJN92_RS13390 (EJN92_13390) - 3061849..3062157 (-) 309 WP_126128292.1 YqjD family protein -
  EJN92_RS13395 (EJN92_13395) ubiA 3062303..3063169 (-) 867 WP_126128293.1 4-hydroxybenzoate octaprenyltransferase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41786.19 Da        Isoelectric Point: 6.7720

>NTDB_id=331490 EJN92_RS13360 WP_126128286.1 3057234..3058370(-) (pilU) [Undibacterium parvum strain DSM 23061]
MERDQATKFMNDLLRLMLSKNGSDLFITSDFPPAFKIDGKVTPVSNQALSASHTVDLARAIMNDKQSAEFESTKECNFAI
NPAGMGRFRVSAFMQQGKVGMVMRTITTAIPKFEDLGLPEQLKEVAMTKRGLVIMVGATGSGKSTSLAAMIGYRNANSYG
HIITIEDPIEYVHPHNNCIITQREVGIDTADWGVALKNTLRQAPDVIQIGEIRERETMDFAIAFAETGHLCLATLHANSA
NQALDRIINFFPEDRRQQLLMDLSLNLKAIVSQRLLPLKAKKGRVAAIEILLNSPLISDLIFKGDVHEIKEIMKKSGELG
MQTFDQALFDLHEADMITYEDALRNADSVNELRLSIKLRGKEAKGRDLSAGTSHLGIL

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=331490 EJN92_RS13360 WP_126128286.1 3057234..3058370(-) (pilU) [Undibacterium parvum strain DSM 23061]
ATGGAACGCGATCAGGCCACCAAATTCATGAACGATCTGCTGCGCCTCATGCTCAGCAAGAATGGCTCAGACTTATTTAT
CACCTCCGATTTTCCACCCGCTTTCAAAATCGATGGCAAGGTCACGCCAGTTTCAAATCAAGCGCTATCGGCTTCGCACA
CGGTCGATCTGGCGCGCGCCATCATGAATGATAAACAGTCGGCAGAATTTGAATCGACCAAAGAATGTAATTTTGCGATC
AATCCAGCTGGCATGGGGCGCTTCCGTGTTTCCGCCTTTATGCAGCAGGGTAAAGTCGGGATGGTGATGCGTACCATCAC
CACCGCCATCCCTAAATTTGAAGATCTGGGCCTGCCCGAGCAATTAAAGGAAGTCGCCATGACCAAGCGTGGCTTGGTGA
TCATGGTCGGCGCCACTGGTTCAGGAAAATCCACCTCGCTGGCGGCGATGATAGGCTACCGCAATGCCAACAGCTACGGT
CACATCATCACGATTGAAGATCCAATTGAATACGTGCATCCGCATAATAATTGCATCATCACCCAGCGTGAAGTCGGGAT
CGATACCGCCGACTGGGGCGTTGCGCTGAAGAACACCTTGCGCCAGGCGCCAGACGTGATACAGATAGGCGAAATTCGTG
AACGCGAGACCATGGATTTTGCGATTGCCTTTGCCGAAACCGGACATCTGTGCCTGGCGACTCTGCATGCAAATAGCGCC
AATCAAGCACTCGATCGTATCATCAACTTTTTCCCTGAAGACCGGCGTCAGCAATTGCTGATGGATTTATCCCTGAATCT
AAAAGCCATCGTGTCGCAGCGCCTATTGCCGCTGAAAGCGAAAAAAGGCCGGGTCGCTGCGATCGAGATCTTGCTCAACT
CTCCTTTGATTTCTGATCTGATTTTTAAAGGCGACGTCCACGAGATCAAAGAAATCATGAAAAAATCCGGTGAATTGGGC
ATGCAGACCTTCGATCAGGCGTTGTTTGATTTGCATGAAGCCGACATGATTACCTATGAAGATGCGCTACGTAATGCCGA
CTCCGTCAATGAACTACGACTGTCTATCAAATTACGCGGCAAAGAAGCCAAAGGCCGCGATTTATCAGCGGGCACCAGCC
ATCTGGGCATACTATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(99-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3Q9BRM5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

65.363

94.709

0.619
  pilU Acinetobacter baylyi ADP1

60.446

94.974

0.574
  pilU Vibrio cholerae strain A1552

54.821

96.032

0.526
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.51

89.153

0.397
  pilT Pseudomonas stutzeri DSM 10701

41.246

89.153

0.368
  pilT Pseudomonas aeruginosa PAK

40.95

89.153

0.365
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.81

83.333

0.365
  pilT Vibrio cholerae strain A1552

43.81

83.333

0.365
  pilT Legionella pneumophila strain ERS1305867

41.692

87.566

0.365
  pilT Legionella pneumophila strain Lp02

41.692

87.566

0.365

Multiple sequence alignment