Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   D0436_RS15430 Genome accession   NZ_CP031775
Coordinates   3539674..3540711 (-) Length   345 a.a.
NCBI ID   WP_023266515.1    Uniprot ID   A0A5B8QWG8
Organism   Shewanella decolorationis strain Ni1-3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3534674..3545711
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D0436_RS15395 (D0436_07195) - 3534846..3535160 (+) 315 WP_023266510.1 hypothetical protein -
  D0436_RS15400 (D0436_07190) yciH 3535310..3535639 (+) 330 WP_208662024.1 stress response translation initiation inhibitor YciH -
  D0436_RS15405 (D0436_07185) - 3535708..3536271 (+) 564 WP_133178770.1 YqgE/AlgH family protein -
  D0436_RS15410 (D0436_07180) ruvX 3536302..3536727 (+) 426 WP_011621986.1 Holliday junction resolvase RuvX -
  D0436_RS15415 (D0436_07175) hemH 3536789..3537772 (-) 984 WP_133178769.1 ferrochelatase -
  D0436_RS15420 (D0436_07170) - 3537842..3538375 (-) 534 WP_086904528.1 glutathione peroxidase -
  D0436_RS15425 (D0436_07165) pilU 3538552..3539664 (-) 1113 WP_039977794.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  D0436_RS15430 (D0436_07160) pilT 3539674..3540711 (-) 1038 WP_023266515.1 type IV pilus twitching motility protein PilT Machinery gene
  D0436_RS15435 (D0436_07155) - 3540747..3541463 (+) 717 WP_133178767.1 YggS family pyridoxal phosphate-dependent enzyme -
  D0436_RS23595 - 3541460..3541591 (-) 132 WP_302852162.1 hypothetical protein -
  D0436_RS15440 (D0436_07150) proC 3541688..3542506 (+) 819 WP_208662022.1 pyrroline-5-carboxylate reductase -
  D0436_RS15445 (D0436_07145) - 3542592..3543140 (+) 549 WP_023266519.1 YggT family protein -
  D0436_RS15450 (D0436_07140) yggU 3543140..3543430 (+) 291 WP_133178765.1 DUF167 family protein YggU -
  D0436_RS15455 (D0436_07135) - 3543499..3543933 (+) 435 WP_039977795.1 DUF4426 domain-containing protein -
  D0436_RS15460 (D0436_07130) rdgB 3544090..3544707 (+) 618 WP_011625625.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38310.00 Da        Isoelectric Point: 6.9096

>NTDB_id=311142 D0436_RS15430 WP_023266515.1 3539674..3540711(-) (pilT) [Shewanella decolorationis strain Ni1-3]
MEITELLAFSVKHKASDLHLSAGISPMIRVDGEVRKINLPALDHQGVHSLVYDIMNDKQRKDFEEHLEIDFSFEVPNLAR
FRVNAFNQSRGAAAVFRTIPSDILSLEQLGAPEIFKKIASFPRGLVLVTGPTGSGKSTTLAAMVDYINENRHDHILTIED
PIEFVHQNKQCLINQREVHRHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAGEKDMVRTMLSESLQAVISQTLIKKIGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLEQCL
QNLVNRGLITREDAMAKSSNKQATF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=311142 D0436_RS15430 WP_023266515.1 3539674..3540711(-) (pilT) [Shewanella decolorationis strain Ni1-3]
ATGGAAATCACTGAGTTATTAGCCTTTAGTGTAAAACACAAAGCCTCGGATCTACACCTCTCTGCAGGGATATCTCCCAT
GATCCGTGTCGACGGTGAAGTGAGAAAAATTAACCTGCCCGCGCTCGATCATCAGGGGGTACACAGCCTTGTGTACGACA
TTATGAATGATAAACAGCGTAAGGACTTTGAAGAGCATTTAGAAATCGACTTTTCGTTCGAAGTCCCTAATTTAGCGCGT
TTCCGTGTGAACGCCTTTAACCAATCCCGCGGTGCGGCGGCGGTATTTCGTACCATTCCTAGTGATATATTGTCACTTGA
GCAGCTAGGTGCGCCTGAAATTTTTAAAAAAATCGCCAGTTTTCCCCGTGGCTTAGTGTTAGTCACTGGCCCTACTGGTT
CTGGTAAGAGTACCACACTTGCGGCCATGGTGGATTACATCAATGAGAACCGCCACGACCATATCTTAACCATTGAAGAT
CCTATCGAATTCGTTCACCAGAATAAGCAATGTTTGATTAACCAACGGGAAGTGCATCGTCATACCCACAGCTTTAACGC
TGCGCTACGTAGTGCACTGCGTGAAGACCCTGACGTTATCCTCGTCGGTGAGATGCGTGACCTTGAAACCATTCGTCTGG
CGATGACGGCGGCCGAAACGGGTCACTTAGTTTTTGGTACCTTGCACACCACCTCGGCGGCTAAGACCATCGACCGTGTG
GTTGACGTGTTCCCTGCCGGTGAAAAGGACATGGTGCGTACCATGTTGTCTGAATCATTACAGGCGGTTATTTCGCAAAC
TCTGATTAAGAAAATCGGTGGTGGCCGTGTGGCAGCTCACGAAATCATGATGGGAACGCCCGCCATCCGTAACCTTATCC
GTGAAGATAAAGTGGCGCAGATGTACTCAGCTATTCAAACGGGGATGGCCCATGGCATGCAAACGCTTGAACAGTGCCTG
CAAAACTTAGTCAACCGTGGTCTCATCACCCGTGAGGATGCCATGGCGAAGAGCTCAAACAAACAAGCGACCTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5B8QWG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

80.409

99.13

0.797
  pilT Acinetobacter baumannii strain A118

80.409

99.13

0.797
  pilT Acinetobacter nosocomialis M2

80.409

99.13

0.797
  pilT Pseudomonas aeruginosa PAK

79.29

97.971

0.777
  pilT Acinetobacter baylyi ADP1

77.843

99.42

0.774
  pilT Pseudomonas stutzeri DSM 10701

77.485

99.13

0.768
  pilT Legionella pneumophila strain Lp02

75.073

98.841

0.742
  pilT Legionella pneumophila strain ERS1305867

75.073

98.841

0.742
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.404

97.681

0.707
  pilT Vibrio cholerae strain A1552

72.404

97.681

0.707
  pilT Neisseria meningitidis 8013

69.767

99.71

0.696
  pilT Neisseria gonorrhoeae MS11

69.477

99.71

0.693
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.147

98.261

0.493
  pilU Pseudomonas stutzeri DSM 10701

42.274

99.42

0.42
  pilU Vibrio cholerae strain A1552

41.691

99.42

0.414
  pilU Acinetobacter baylyi ADP1

40.059

97.681

0.391

Multiple sequence alignment