Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   D0436_RS15425 Genome accession   NZ_CP031775
Coordinates   3538552..3539664 (-) Length   370 a.a.
NCBI ID   WP_039977794.1    Uniprot ID   A0A5B8QW01
Organism   Shewanella decolorationis strain Ni1-3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3533552..3544664
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D0436_RS15390 (D0436_07200) - 3534106..3534666 (-) 561 WP_023266509.1 DUF4136 domain-containing protein -
  D0436_RS15395 (D0436_07195) - 3534846..3535160 (+) 315 WP_023266510.1 hypothetical protein -
  D0436_RS15400 (D0436_07190) yciH 3535310..3535639 (+) 330 WP_208662024.1 stress response translation initiation inhibitor YciH -
  D0436_RS15405 (D0436_07185) - 3535708..3536271 (+) 564 WP_133178770.1 YqgE/AlgH family protein -
  D0436_RS15410 (D0436_07180) ruvX 3536302..3536727 (+) 426 WP_011621986.1 Holliday junction resolvase RuvX -
  D0436_RS15415 (D0436_07175) hemH 3536789..3537772 (-) 984 WP_133178769.1 ferrochelatase -
  D0436_RS15420 (D0436_07170) - 3537842..3538375 (-) 534 WP_086904528.1 glutathione peroxidase -
  D0436_RS15425 (D0436_07165) pilU 3538552..3539664 (-) 1113 WP_039977794.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  D0436_RS15430 (D0436_07160) pilT 3539674..3540711 (-) 1038 WP_023266515.1 type IV pilus twitching motility protein PilT Machinery gene
  D0436_RS15435 (D0436_07155) - 3540747..3541463 (+) 717 WP_133178767.1 YggS family pyridoxal phosphate-dependent enzyme -
  D0436_RS23595 - 3541460..3541591 (-) 132 WP_302852162.1 hypothetical protein -
  D0436_RS15440 (D0436_07150) proC 3541688..3542506 (+) 819 WP_208662022.1 pyrroline-5-carboxylate reductase -
  D0436_RS15445 (D0436_07145) - 3542592..3543140 (+) 549 WP_023266519.1 YggT family protein -
  D0436_RS15450 (D0436_07140) yggU 3543140..3543430 (+) 291 WP_133178765.1 DUF167 family protein YggU -
  D0436_RS15455 (D0436_07135) - 3543499..3543933 (+) 435 WP_039977795.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41171.42 Da        Isoelectric Point: 6.3728

>NTDB_id=311141 D0436_RS15425 WP_039977794.1 3538552..3539664(-) (pilU) [Shewanella decolorationis strain Ni1-3]
MDVRPFLKVMVERKASDLFITAGFPPSAKIDGELRPLAESAFTPAQSLDFVESVMTEAQKKEFHTTRECNFAFAVKELGR
FRVSAFWQRESPGCVMRRIETKIPEVEDLKLPPILKDLVMSKRGLIIMVGGTGTGKSTSLAALVGYRNAHARGHILTIED
PVEFVHDHRKSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHQQLLFDLSLNLRGIVAQQLVPKVDGTGRRAAIEVLINTPRVASLIAKNELHLLKETMAKSNEQGMQTFDQA
LLQLYIDGEISYADALHHADSPNDLRLMIKLQNKEPASSSFMEGVTLDMD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=311141 D0436_RS15425 WP_039977794.1 3538552..3539664(-) (pilU) [Shewanella decolorationis strain Ni1-3]
ATGGATGTCCGTCCGTTTTTAAAAGTCATGGTGGAGCGTAAAGCCTCGGACTTGTTTATTACCGCAGGTTTTCCGCCGAG
CGCCAAAATCGATGGTGAGTTACGCCCATTAGCCGAGAGTGCCTTCACGCCCGCGCAGTCTTTGGATTTTGTTGAATCCG
TGATGACTGAGGCGCAAAAGAAGGAGTTTCACACCACTCGCGAGTGTAACTTTGCTTTTGCAGTGAAGGAGTTAGGTCGT
TTTCGCGTGAGTGCATTCTGGCAGCGTGAATCTCCAGGATGCGTGATGCGCCGGATTGAGACCAAAATCCCTGAGGTGGA
AGACTTAAAACTGCCACCGATTTTAAAAGATCTGGTGATGAGTAAACGGGGTCTTATCATCATGGTTGGGGGAACGGGAA
CTGGTAAGTCGACCTCCTTGGCGGCATTAGTGGGTTATCGTAATGCCCATGCCCGTGGTCATATCCTCACCATCGAAGAC
CCGGTGGAATTTGTGCATGACCATCGCAAAAGTATCATTACCCAACGAGAAGTCGGAATTGATACAGAGTCCTTTGATGC
GGCGCTGAAGAGTTCGCTGCGTCAGGCGCCCGATGTGATTTTGATTGGTGAGATCCGTACTCAAGAAACCATGGAGTTTG
CGCTTTCTTTCGCCGAAACGGGTCACCTCTGTATGGCGACATTGCACGCGAACAACGCTAACCAAGCGTTAGACCGGATC
ATGCACTTAGTGCCTGAGAGTAAACACCAGCAGTTGTTATTCGACTTGTCACTCAACCTGCGCGGCATTGTGGCGCAGCA
ACTGGTGCCTAAGGTTGATGGCACAGGACGACGGGCGGCGATTGAAGTCTTGATCAATACGCCACGTGTCGCGAGTTTGA
TTGCTAAAAACGAGCTGCATTTGCTTAAAGAAACCATGGCTAAATCGAATGAGCAGGGGATGCAGACCTTCGACCAAGCT
TTGCTGCAACTCTATATCGATGGGGAAATCAGCTATGCCGATGCGCTTCACCATGCTGACTCGCCAAACGACTTACGTTT
AATGATCAAATTGCAAAATAAAGAGCCCGCCAGTTCAAGCTTTATGGAAGGCGTGACCTTGGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5B8QW01

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.377

100

0.605
  pilU Vibrio cholerae strain A1552

58.919

100

0.589
  pilU Acinetobacter baylyi ADP1

60.795

95.135

0.578
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.333

89.189

0.386
  pilT Vibrio cholerae strain A1552

43.333

89.189

0.386
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.58

93.243

0.378
  pilT Acinetobacter nosocomialis M2

39.766

92.432

0.368
  pilT Acinetobacter baumannii D1279779

39.766

92.432

0.368
  pilT Acinetobacter baumannii strain A118

39.766

92.432

0.368
  pilT Acinetobacter baylyi ADP1

39.474

92.432

0.365
  pilT Legionella pneumophila strain Lp02

41.358

87.568

0.362
  pilT Legionella pneumophila strain ERS1305867

41.358

87.568

0.362

Multiple sequence alignment