Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   D0B54_RS17215 Genome accession   NZ_CP031704
Coordinates   3830087..3831130 (-) Length   347 a.a.
NCBI ID   WP_117292494.1    Uniprot ID   A0A346N417
Organism   Solimonas sp. K1W22B-7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3825087..3836130
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D0B54_RS17180 (D0B54_17180) - 3825468..3826025 (-) 558 WP_117292488.1 DUF3228 family protein -
  D0B54_RS17190 (D0B54_17190) rpsI 3826225..3826623 (-) 399 WP_117292489.1 30S ribosomal protein S9 -
  D0B54_RS17195 (D0B54_17195) rplM 3826636..3827064 (-) 429 WP_117292490.1 50S ribosomal protein L13 -
  D0B54_RS17200 (D0B54_17200) - 3827259..3827759 (+) 501 WP_117292491.1 hypothetical protein -
  D0B54_RS17205 (D0B54_17205) pilU 3827787..3828848 (-) 1062 WP_117292492.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  D0B54_RS17210 (D0B54_17210) pilU 3828888..3830054 (-) 1167 WP_117292493.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  D0B54_RS17215 (D0B54_17215) pilT 3830087..3831130 (-) 1044 WP_117292494.1 type IV pilus twitching motility protein PilT Machinery gene
  D0B54_RS17220 (D0B54_17220) - 3831200..3831886 (+) 687 WP_117292495.1 YggS family pyridoxal phosphate-dependent enzyme -
  D0B54_RS17225 (D0B54_17225) rpiA 3831883..3832545 (+) 663 WP_117292496.1 ribose-5-phosphate isomerase RpiA -
  D0B54_RS17230 (D0B54_17230) - 3832647..3832919 (-) 273 WP_117292497.1 RNA-binding protein -
  D0B54_RS17235 (D0B54_17235) - 3832988..3834325 (-) 1338 WP_117292498.1 DEAD/DEAH box helicase -
  D0B54_RS17240 (D0B54_17240) - 3834638..3835042 (+) 405 WP_117295337.1 DUF4399 domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38534.39 Da        Isoelectric Point: 7.0676

>NTDB_id=310635 D0B54_RS17215 WP_117292494.1 3830087..3831130(-) (pilT) [Solimonas sp. K1W22B-7]
MDIAQLLTFSVKQGASDLHLSAGVEPMIRVDGDVKRINLPPLDHKQVHDMVYDIMNDKQRKAWDEFLETDFSFEIPGLAR
FRVNAFTHARGSGAVFRTIPSKILSLEELKCPSIFKDIAEYPRGVVLVTGPTGSGKSTTLAGIVNHINENEYGHILTIED
PIEFVHQSKKCLINQREVHRDTLGFNESLRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAAEKEMVRAMLSESLRAVISQTLLKKKGGGRVAAHEIMIGNPAIRNLIREAKIAQMYSAIQTGQKEGMQTLDQCL
KDMVKRGIVGLEEARIKSADPRNFTVN

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=310635 D0B54_RS17215 WP_117292494.1 3830087..3831130(-) (pilT) [Solimonas sp. K1W22B-7]
ATGGATATCGCGCAGTTGCTGACTTTCAGCGTCAAACAGGGCGCGTCAGACCTGCACCTTTCCGCTGGCGTCGAGCCGAT
GATCCGGGTCGACGGCGACGTCAAGCGCATCAACCTGCCGCCGCTGGACCACAAACAGGTCCACGACATGGTGTACGACA
TCATGAACGACAAGCAGCGCAAGGCCTGGGACGAATTCCTCGAAACCGACTTCTCGTTCGAAATCCCCGGCCTGGCGCGC
TTCCGCGTCAACGCCTTCACCCATGCGCGCGGCTCGGGCGCCGTGTTCCGTACCATTCCCTCCAAGATCCTGTCGCTGGA
GGAGCTCAAGTGCCCGTCCATCTTCAAGGACATCGCCGAGTATCCGCGCGGCGTGGTGCTGGTCACCGGCCCGACCGGCT
CGGGCAAGTCCACGACCCTGGCCGGCATCGTCAACCACATCAACGAGAACGAGTACGGTCACATCCTCACCATCGAGGAT
CCGATCGAATTCGTGCACCAGTCGAAGAAGTGCCTGATCAACCAGCGCGAAGTGCACCGCGACACGCTGGGCTTCAACGA
GTCGCTGCGCTCGGCCTTGCGTGAAGACCCCGACGTCATCCTGGTCGGCGAAATGCGCGACCTGGAAACGATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGACACCTGGTGTTCGGTACCCTGCATACCTCGTCGGCGGCCAAGACCATCGACCGTATC
ATCGACGTGTTCCCGGCGGCGGAAAAGGAAATGGTGCGCGCGATGCTGTCCGAATCGCTGCGTGCGGTGATCTCGCAGAC
CCTGCTCAAGAAGAAGGGCGGCGGCCGTGTCGCGGCGCACGAGATCATGATCGGCAACCCGGCCATCCGCAACCTGATCC
GCGAGGCCAAGATCGCGCAGATGTACTCGGCCATCCAGACCGGCCAGAAAGAGGGCATGCAGACACTGGACCAGTGCCTC
AAGGACATGGTCAAGAGAGGTATCGTCGGGCTGGAAGAAGCACGCATCAAGTCCGCCGATCCGCGTAACTTCACCGTCAA
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A346N417

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.616

99.135

0.769
  pilT Acinetobacter baumannii D1279779

77.616

99.135

0.769
  pilT Acinetobacter baumannii strain A118

77.616

99.135

0.769
  pilT Pseudomonas aeruginosa PAK

76.744

99.135

0.761
  pilT Pseudomonas stutzeri DSM 10701

75.291

99.135

0.746
  pilT Acinetobacter baylyi ADP1

75.291

99.135

0.746
  pilT Legionella pneumophila strain Lp02

73.03

95.101

0.695
  pilT Legionella pneumophila strain ERS1305867

73.03

95.101

0.695
  pilT Neisseria meningitidis 8013

67.919

99.712

0.677
  pilT Neisseria gonorrhoeae MS11

67.63

99.712

0.674
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.546

97.118

0.666
  pilT Vibrio cholerae strain A1552

68.546

97.118

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.855

99.424

0.496
  pilU Acinetobacter baylyi ADP1

40.58

99.424

0.403
  pilU Pseudomonas stutzeri DSM 10701

40.116

99.135

0.398
  pilU Vibrio cholerae strain A1552

38.953

99.135

0.386

Multiple sequence alignment