Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   D0B54_RS17210 Genome accession   NZ_CP031704
Coordinates   3828888..3830054 (-) Length   388 a.a.
NCBI ID   WP_117292493.1    Uniprot ID   A0A346N416
Organism   Solimonas sp. K1W22B-7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3823888..3835054
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D0B54_RS17175 (D0B54_17175) - 3824550..3825233 (-) 684 WP_117292487.1 carbonic anhydrase -
  D0B54_RS17180 (D0B54_17180) - 3825468..3826025 (-) 558 WP_117292488.1 DUF3228 family protein -
  D0B54_RS17190 (D0B54_17190) rpsI 3826225..3826623 (-) 399 WP_117292489.1 30S ribosomal protein S9 -
  D0B54_RS17195 (D0B54_17195) rplM 3826636..3827064 (-) 429 WP_117292490.1 50S ribosomal protein L13 -
  D0B54_RS17200 (D0B54_17200) - 3827259..3827759 (+) 501 WP_117292491.1 hypothetical protein -
  D0B54_RS17205 (D0B54_17205) pilU 3827787..3828848 (-) 1062 WP_117292492.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  D0B54_RS17210 (D0B54_17210) pilU 3828888..3830054 (-) 1167 WP_117292493.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  D0B54_RS17215 (D0B54_17215) pilT 3830087..3831130 (-) 1044 WP_117292494.1 type IV pilus twitching motility protein PilT Machinery gene
  D0B54_RS17220 (D0B54_17220) - 3831200..3831886 (+) 687 WP_117292495.1 YggS family pyridoxal phosphate-dependent enzyme -
  D0B54_RS17225 (D0B54_17225) rpiA 3831883..3832545 (+) 663 WP_117292496.1 ribose-5-phosphate isomerase RpiA -
  D0B54_RS17230 (D0B54_17230) - 3832647..3832919 (-) 273 WP_117292497.1 RNA-binding protein -
  D0B54_RS17235 (D0B54_17235) - 3832988..3834325 (-) 1338 WP_117292498.1 DEAD/DEAH box helicase -
  D0B54_RS17240 (D0B54_17240) - 3834638..3835042 (+) 405 WP_117295337.1 DUF4399 domain-containing protein -

Sequence

Protein


Download         Length: 388 a.a.        Molecular weight: 43579.19 Da        Isoelectric Point: 6.8462

>NTDB_id=310634 D0B54_RS17210 WP_117292493.1 3828888..3830054(-) (pilU) [Solimonas sp. K1W22B-7]
MERDQAIKLVQQLLTLMKQKGASDLFISSGFPPAIKLDGQMTPVMDKPLQPEQAAIIMRSLMNDRNIKEFEASNECNFAI
SPPGIGRFRVNAFVQQGRVGGVMRTINTTIPTFEQLGLPEQLKKVCMEKRGLVLMVGATGSGKSTSLAAMVGHRNANSRG
HIITIEDPIEYVHPHNGCMVTQREVGQDTFSWENALKNTLRQAPDVIQIGEIRTRETMEYAVNFAETGHLVLSTLHANSA
NQALDRIINFFPEEKREQLLMDLSLNLKAIISQRLVRKDGGGRAAAMEIMINSPLVADMIFKGDVVGIKEVMGRSNEQGM
ITFDQYLFELFEDGVISYDEAVRNADSQNELRLRVKLESRRARQNLMDDDMVRKMGMKEEEKSQLVHR

Nucleotide


Download         Length: 1167 bp        

>NTDB_id=310634 D0B54_RS17210 WP_117292493.1 3828888..3830054(-) (pilU) [Solimonas sp. K1W22B-7]
ATGGAACGCGACCAGGCAATCAAACTCGTACAGCAGTTGCTGACCCTGATGAAGCAGAAGGGTGCATCCGATCTGTTCAT
CTCCTCCGGCTTCCCGCCGGCGATCAAGCTCGACGGCCAGATGACGCCGGTGATGGACAAGCCGCTGCAGCCCGAGCAGG
CGGCCATCATCATGCGTTCGCTGATGAACGACCGGAACATCAAGGAGTTCGAAGCCAGCAACGAGTGCAACTTCGCGATC
AGCCCGCCGGGTATCGGCCGCTTCCGCGTCAACGCCTTCGTGCAGCAGGGCCGTGTCGGCGGCGTGATGCGTACCATCAA
CACCACGATCCCCACCTTCGAGCAGCTGGGCCTGCCCGAGCAGCTCAAGAAGGTCTGCATGGAAAAGCGCGGCCTGGTGC
TGATGGTCGGCGCCACGGGTTCGGGCAAGTCGACCTCGCTGGCGGCGATGGTGGGCCACCGCAACGCCAATTCGCGCGGC
CACATCATCACGATCGAGGACCCGATCGAGTACGTGCATCCGCACAACGGCTGCATGGTGACCCAGCGCGAAGTCGGCCA
GGATACCTTCTCCTGGGAAAACGCCCTGAAGAACACCCTGCGCCAGGCGCCCGACGTCATCCAGATCGGCGAAATCCGTA
CCCGCGAGACCATGGAGTACGCGGTCAACTTCGCCGAAACCGGTCACCTCGTGCTGTCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGCATCATCAACTTCTTCCCCGAGGAGAAGCGCGAGCAGCTGCTGATGGACCTGTCGCTGAACCT
CAAGGCGATCATTTCCCAGCGCCTGGTGCGCAAGGACGGCGGCGGCCGTGCCGCGGCCATGGAAATCATGATCAATTCGC
CGCTGGTCGCCGACATGATCTTCAAGGGCGACGTGGTCGGCATCAAGGAAGTCATGGGCCGCTCCAACGAGCAGGGCATG
ATCACCTTCGACCAGTACCTGTTCGAGCTGTTCGAGGACGGCGTCATCAGCTACGACGAGGCGGTCCGCAACGCCGACTC
GCAGAACGAGCTGCGCCTGCGCGTCAAGCTCGAGTCCCGTCGTGCACGCCAGAACCTGATGGATGACGACATGGTGCGCA
AGATGGGCATGAAGGAAGAAGAAAAATCGCAGCTGGTGCACCGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A346N416

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.664

92.01

0.549
  pilU Acinetobacter baylyi ADP1

55.62

89.433

0.497
  pilU Vibrio cholerae strain A1552

53.448

89.691

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.481

88.402

0.402
  pilT Acinetobacter baumannii strain A118

42.815

87.887

0.376
  pilT Acinetobacter nosocomialis M2

42.815

87.887

0.376
  pilT Acinetobacter baumannii D1279779

42.815

87.887

0.376
  pilT Pseudomonas stutzeri DSM 10701

43.452

86.598

0.376
  pilT Pseudomonas aeruginosa PAK

43.452

86.598

0.376
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.598

85.309

0.363
  pilT Vibrio cholerae strain A1552

42.598

85.309

0.363

Multiple sequence alignment