Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   D0B54_RS17205 Genome accession   NZ_CP031704
Coordinates   3827787..3828848 (-) Length   353 a.a.
NCBI ID   WP_117292492.1    Uniprot ID   A0A346N415
Organism   Solimonas sp. K1W22B-7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3822787..3833848
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D0B54_RS17165 (D0B54_17165) - 3822867..3823592 (-) 726 WP_117292485.1 TetR/AcrR family transcriptional regulator -
  D0B54_RS17170 (D0B54_17170) phbB 3823803..3824549 (-) 747 WP_117292486.1 acetoacetyl-CoA reductase -
  D0B54_RS17175 (D0B54_17175) - 3824550..3825233 (-) 684 WP_117292487.1 carbonic anhydrase -
  D0B54_RS17180 (D0B54_17180) - 3825468..3826025 (-) 558 WP_117292488.1 DUF3228 family protein -
  D0B54_RS17190 (D0B54_17190) rpsI 3826225..3826623 (-) 399 WP_117292489.1 30S ribosomal protein S9 -
  D0B54_RS17195 (D0B54_17195) rplM 3826636..3827064 (-) 429 WP_117292490.1 50S ribosomal protein L13 -
  D0B54_RS17200 (D0B54_17200) - 3827259..3827759 (+) 501 WP_117292491.1 hypothetical protein -
  D0B54_RS17205 (D0B54_17205) pilU 3827787..3828848 (-) 1062 WP_117292492.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  D0B54_RS17210 (D0B54_17210) pilU 3828888..3830054 (-) 1167 WP_117292493.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  D0B54_RS17215 (D0B54_17215) pilT 3830087..3831130 (-) 1044 WP_117292494.1 type IV pilus twitching motility protein PilT Machinery gene
  D0B54_RS17220 (D0B54_17220) - 3831200..3831886 (+) 687 WP_117292495.1 YggS family pyridoxal phosphate-dependent enzyme -
  D0B54_RS17225 (D0B54_17225) rpiA 3831883..3832545 (+) 663 WP_117292496.1 ribose-5-phosphate isomerase RpiA -
  D0B54_RS17230 (D0B54_17230) - 3832647..3832919 (-) 273 WP_117292497.1 RNA-binding protein -

Sequence

Protein


Download         Length: 353 a.a.        Molecular weight: 39524.55 Da        Isoelectric Point: 6.7777

>NTDB_id=310633 D0B54_RS17205 WP_117292492.1 3827787..3828848(-) (pilU) [Solimonas sp. K1W22B-7]
MLKILPYLKLAVEKNASDIFFTANAPAMLKIEGEMHAVGKTLMTSEFIRELAYSILTPEQQAHLEEHWELDLATQAGGLG
RFRVNIFNQRGTLAMVLRYVRSQVPTIAELGVPEVLGDLIMLKRGLVLMVGATGSGKSTTLAAMINHRNELSSGHILTIE
DPVEFVHPNRRSIVNQREVGTDTITYERALKSSLREAPDMILIGEVRVRETMDACIQLANTGHLAVSTLHANNAYQALQR
IVNLYPPDLRDQLYMDLSLTLRAIISQRLVRRKDGRRVPAFEVMINSPYVQELILHKRIDEIKDAMNQSSDKGTLTFDAS
LYGLYRQGVIELDEALNNADSRTNLEAKINFGS

Nucleotide


Download         Length: 1062 bp        

>NTDB_id=310633 D0B54_RS17205 WP_117292492.1 3827787..3828848(-) (pilU) [Solimonas sp. K1W22B-7]
ATGCTGAAGATCCTGCCGTACCTGAAACTGGCGGTGGAGAAGAACGCCTCCGACATTTTCTTCACCGCCAACGCGCCGGC
GATGTTGAAGATCGAGGGCGAGATGCACGCCGTCGGCAAGACCCTGATGACCTCCGAGTTCATCCGGGAGCTGGCCTACA
GCATCCTGACCCCCGAGCAGCAGGCGCACCTGGAAGAGCACTGGGAGCTGGACCTGGCGACGCAGGCCGGCGGCCTGGGC
CGTTTCCGCGTCAACATCTTCAACCAGCGCGGCACGCTCGCCATGGTGCTGCGCTACGTCAGGTCGCAGGTGCCGACGAT
CGCCGAGCTGGGGGTGCCCGAGGTGCTGGGCGACCTGATCATGCTCAAGCGCGGCCTGGTCCTGATGGTCGGCGCTACCG
GCTCGGGCAAGTCCACCACGCTGGCGGCGATGATCAATCATCGCAACGAGCTGTCCAGCGGCCACATCCTGACGATCGAG
GATCCGGTGGAGTTCGTCCACCCGAACCGCCGCAGCATCGTCAACCAGCGTGAAGTCGGCACCGACACCATCACCTACGA
GCGCGCGCTGAAGAGCTCGCTGCGCGAGGCGCCGGACATGATCCTGATCGGCGAGGTACGTGTCCGCGAAACCATGGATG
CCTGCATCCAGCTGGCCAACACCGGTCACCTGGCGGTATCGACGCTGCACGCCAACAACGCCTACCAGGCGCTGCAGCGT
ATCGTGAACCTGTACCCGCCCGACTTGCGCGACCAGCTCTACATGGACCTGTCGCTGACCCTGCGCGCGATCATTTCGCA
GCGCCTGGTGCGCCGCAAGGACGGTCGCCGCGTGCCGGCCTTCGAGGTCATGATCAACTCTCCCTACGTGCAGGAACTGA
TCCTCCACAAGCGCATCGACGAGATCAAGGACGCCATGAACCAGTCCTCGGACAAGGGCACGCTGACCTTCGACGCCTCG
CTCTATGGGCTCTACCGCCAGGGCGTGATCGAACTCGACGAGGCGCTCAACAACGCCGATTCGCGCACGAATCTGGAAGC
CAAGATCAACTTCGGCAGCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A346N415

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.009

98.3

0.501
  pilU Vibrio cholerae strain A1552

47.647

96.317

0.459
  pilU Acinetobacter baylyi ADP1

45.533

98.3

0.448
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.953

96.034

0.422
  pilT Acinetobacter baylyi ADP1

39.412

96.317

0.38
  pilT Pseudomonas aeruginosa PAK

38.081

97.45

0.371
  pilT Pseudomonas stutzeri DSM 10701

38.081

97.45

0.371
  pilT Acinetobacter baumannii D1279779

38.906

93.201

0.363
  pilT Acinetobacter baumannii strain A118

38.906

93.201

0.363
  pilT Acinetobacter nosocomialis M2

38.906

93.201

0.363

Multiple sequence alignment