NTDB

Overview

Name	pilT	Type	Machinery gene
Locus tag	AB849_RS13045	Genome accession	NZ_CP036487
Coordinates	2555374..2555772 (-)	Length	132 a.a.
NCBI ID	WP_084712858.1	Uniprot ID	-
Organism	Thermoactinomyces vulgaris strain CDF
Function	power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2550374..2560772

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AB849_RS13020 (AB849_013020)	-	2550822..2551880 (-)	1059	WP_037993509.1	rod shape-determining protein	-
AB849_RS13025 (AB849_013025)	ysxA/radC	2551924..2552631 (-)	708	WP_037993511.1	RadC family protein	Machinery gene
AB849_RS13030 (AB849_013030)	-	2552721..2553320 (-)	600	WP_037993512.1	Maf family protein	-
AB849_RS13035 (AB849_013035)	-	2553517..2554632 (-)	1116	WP_037993514.1	SPOR domain-containing protein	-
AB849_RS13040 (AB849_013040)	-	2554680..2555381 (-)	702	WP_037993517.1	prepilin peptidase	-
AB849_RS13045 (AB849_013045)	pilT	2555374..2555772 (-)	399	WP_084712858.1	ATPase, T2SS/T4P/T4SS family	Machinery gene
AB849_RS13050 (AB849_013050)	pilT	2555775..2556344 (-)	570	WP_165489751.1	ATPase, T2SS/T4P/T4SS family	Machinery gene
AB849_RS13055 (AB849_013055)	pptA	2556510..2557244 (+)	735	WP_049719902.1	ABC transporter ATP-binding protein	Regulator
AB849_RS13060 (AB849_013060)	-	2557241..2558470 (+)	1230	WP_037993520.1	ABC transporter permease	-
AB849_RS13065 (AB849_013065)	-	2558501..2559142 (-)	642	WP_037993522.1	SCO family protein	-
AB849_RS13070 (AB849_013070)	murC	2559242..2560627 (-)	1386	WP_037993770.1	UDP-N-acetylmuramate--L-alanine ligase	-

Sequence

Protein

Download Length: 132 a.a. Molecular weight: 14745.04 Da Isoelectric Point: 9.1534

                                >NTDB_id=307940 AB849_RS13045 WP_084712858.1 2555374..2555772(-) (pilT) [Thermoactinomyces vulgaris strain CDF]

MVGELRDTESIMAALHLAETGKRVMATFHAGNVEKAIYRVIDSFPADRQRWVRSQLAASLSAVVAQKMAGPNGRQKRPVF

EVLLNHRSVAHLIRNGEIHQLPSVMQAHQASGMQTMEMELRKLQEEGISCIE

Nucleotide

Download Length: 399 bp

                                >NTDB_id=307940 AB849_RS13045 WP_084712858.1 2555374..2555772(-) (pilT) [Thermoactinomyces vulgaris strain CDF]

ATGGTCGGGGAGTTAAGAGACACTGAAAGCATCATGGCCGCCCTTCACCTTGCCGAAACGGGGAAACGGGTCATGGCCAC

TTTCCATGCCGGGAATGTGGAAAAAGCGATTTACCGGGTCATTGACAGTTTTCCCGCGGATCGGCAAAGATGGGTGCGGT

CACAACTGGCTGCTTCGCTGTCGGCAGTGGTGGCACAAAAGATGGCCGGACCAAACGGCAGGCAGAAGCGGCCAGTGTTT

GAAGTTTTGTTGAACCATCGTTCTGTTGCCCATCTGATTCGAAATGGCGAGATCCACCAGCTCCCTTCCGTGATGCAGGC

TCATCAGGCTTCAGGCATGCAGACCATGGAAATGGAATTGAGAAAATTGCAAGAGGAAGGAATCAGTTGCATTGAATGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(1-63)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilT	Neisseria gonorrhoeae MS11	41.353	100	0.417
pilT	Neisseria meningitidis 8013	41.353	100	0.417
pilU	Pseudomonas stutzeri DSM 10701	42.188	96.97	0.409
pilT	Vibrio cholerae strain A1552	41.406	96.97	0.402
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	41.406	96.97	0.402
pilT	Legionella pneumophila strain Lp02	41.406	96.97	0.402
pilT	Legionella pneumophila strain ERS1305867	41.406	96.97	0.402
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.129	93.939	0.386
pilU	Vibrio cholerae strain A1552	38.71	93.939	0.364
pilT	Pseudomonas stutzeri DSM 10701	37.5	96.97	0.364
pilT	Acinetobacter baylyi ADP1	37.5	96.97	0.364
pilT	Acinetobacter nosocomialis M2	37.5	96.97	0.364
pilT	Pseudomonas aeruginosa PAK	37.5	96.97	0.364
pilT	Acinetobacter baumannii D1279779	37.5	96.97	0.364
pilT	Acinetobacter baumannii strain A118	37.5	96.97	0.364

Detailed information NTG0307940