Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AB849_RS13050 Genome accession   NZ_CP036487
Coordinates   2555775..2556344 (-) Length   189 a.a.
NCBI ID   WP_165489751.1    Uniprot ID   -
Organism   Thermoactinomyces vulgaris strain CDF     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2550775..2561344
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB849_RS13020 (AB849_013020) - 2550822..2551880 (-) 1059 WP_037993509.1 rod shape-determining protein -
  AB849_RS13025 (AB849_013025) ysxA/radC 2551924..2552631 (-) 708 WP_037993511.1 RadC family protein Machinery gene
  AB849_RS13030 (AB849_013030) - 2552721..2553320 (-) 600 WP_037993512.1 Maf family protein -
  AB849_RS13035 (AB849_013035) - 2553517..2554632 (-) 1116 WP_037993514.1 SPOR domain-containing protein -
  AB849_RS13040 (AB849_013040) - 2554680..2555381 (-) 702 WP_037993517.1 prepilin peptidase -
  AB849_RS13045 (AB849_013045) pilT 2555374..2555772 (-) 399 WP_084712858.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  AB849_RS13050 (AB849_013050) pilT 2555775..2556344 (-) 570 WP_165489751.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  AB849_RS13055 (AB849_013055) pptA 2556510..2557244 (+) 735 WP_049719902.1 ABC transporter ATP-binding protein Regulator
  AB849_RS13060 (AB849_013060) - 2557241..2558470 (+) 1230 WP_037993520.1 ABC transporter permease -
  AB849_RS13065 (AB849_013065) - 2558501..2559142 (-) 642 WP_037993522.1 SCO family protein -
  AB849_RS13070 (AB849_013070) murC 2559242..2560627 (-) 1386 WP_037993770.1 UDP-N-acetylmuramate--L-alanine ligase -

Sequence

Protein


Download         Length: 189 a.a.        Molecular weight: 21215.55 Da        Isoelectric Point: 7.8215

>NTDB_id=307941 AB849_RS13050 WP_165489751.1 2555775..2556344(-) (pilT) [Thermoactinomyces vulgaris strain CDF]
MLLEEAVHLALKEGATDLHYIEGEHPCVRVNGKVFVLCAGRGKREEIEKCMASGADPARESDFTWYSGHHRARVHCYQKQ
ERLALAIRFLPDRIPTPKELHLPPVLYQKVMFQTGLLLVTGPTGSGKTTTLASLIQELNRTEALHILTLEDPIEYLHASK
KSLVIQREIGKDTESYVSGMLSALRQGRM

Nucleotide


Download         Length: 570 bp        

>NTDB_id=307941 AB849_RS13050 WP_165489751.1 2555775..2556344(-) (pilT) [Thermoactinomyces vulgaris strain CDF]
ATGCTTTTGGAGGAAGCCGTTCATCTTGCCTTGAAAGAAGGCGCGACCGATTTGCATTACATTGAGGGGGAGCATCCTTG
TGTGCGGGTGAACGGGAAGGTCTTTGTTTTGTGTGCCGGCCGGGGAAAAAGAGAAGAAATTGAAAAGTGCATGGCATCCG
GGGCCGATCCGGCGAGGGAGTCGGATTTCACATGGTATTCAGGTCATCACCGGGCGAGGGTCCATTGTTATCAAAAACAA
GAACGGCTGGCGCTGGCCATCCGCTTTTTGCCGGATCGCATTCCGACTCCGAAAGAGCTTCATCTTCCCCCGGTGCTGTA
TCAAAAAGTGATGTTTCAAACCGGTTTGCTGTTGGTCACGGGCCCGACCGGAAGCGGAAAAACGACAACGCTGGCTTCCT
TGATCCAGGAATTGAATCGTACGGAAGCCTTACATATCCTGACTTTGGAAGATCCGATCGAGTATCTCCATGCGTCAAAA
AAGTCGCTGGTCATCCAACGGGAAATCGGGAAAGACACCGAAAGTTATGTAAGCGGCATGTTGTCCGCTTTGCGGCAAGG
TCGGATGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(114-186)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain Lp02

36.453

100

0.392
  pilT Legionella pneumophila strain ERS1305867

36.453

100

0.392
  pilT Pseudomonas aeruginosa PAK

36.41

100

0.376
  pilT Pseudomonas stutzeri DSM 10701

35.897

100

0.37
  pilU Vibrio cholerae strain A1552

35.897

100

0.37
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

35.751

100

0.365

Multiple sequence alignment