Detailed information NTG0307236

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: DXT74_RS00705
• Genome accession: NZ_CP031514
• Coordinates: 132636..133073 (-)
• Length: 145 a.a.
• NCBI ID: WP_194427089.1
• Uniprot ID: -
• Organism: Chromobacterium sp. Rain0013
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 127636..138073

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DXT74_RS00690 (DXT74_00695)	rpoH	127969..128832 (+)	864	WP_019102027.1	RNA polymerase sigma factor RpoH	-
DXT74_RS00695 (DXT74_00700)	-	128904..130688 (-)	1785	WP_194427087.1	PglL family O-oligosaccharyltransferase	-
DXT74_RS00700 (DXT74_00705)	-	130764..132524 (-)	1761	WP_194427088.1	PglL family O-oligosaccharyltransferase	-
DXT74_RS00705 (DXT74_00710)	pilA	132636..133073 (-)	438	WP_194427089.1	pilin	Machinery gene
DXT74_RS00710 (DXT74_00715)	pilA2	133086..133505 (-)	420	WP_194427090.1	pilin	Machinery gene
DXT74_RS00715 (DXT74_00720)	-	133702..135054 (+)	1353	WP_194427091.1	adenosylmethionine--8-amino-7-oxononanoate transaminase	-
DXT74_RS00720 (DXT74_00725)	-	135156..136658 (+)	1503	WP_194427092.1	hypothetical protein	-
DXT74_RS00725 (DXT74_00730)	proB	136858..137976 (+)	1119	WP_019100517.1	glutamate 5-kinase	-

Sequence

Protein

• Length: 145 a.a.
• Molecular weight: 14821.70 Da
• Isoelectric Point: 8.4688

>NTDB_id=307236 DXT74_RS00705 WP_194427089.1 132636..133073(-) (pilA) [Chromobacterium sp. Rain0013]
MQKSQQGFTLIELMIVVAIIGILAAIAIPAYQDYTKRARVSEGLSLASAAKTAVAEYYASNGSFPSNNTSAGLQASISGN
SVTSVQVGTNGVITVTYSSTLIDTSKNQLTLTPTASGGSIAWTCGASGTTGLNPQWLPANCRAGN

Nucleotide

• Length: 438 bp

>NTDB_id=307236 DXT74_RS00705 WP_194427089.1 132636..133073(-) (pilA) [Chromobacterium sp. Rain0013]
ATGCAAAAATCCCAACAAGGTTTTACTCTTATCGAACTGATGATAGTGGTAGCTATCATTGGTATTCTGGCCGCAATTGC
CATTCCCGCGTATCAGGATTACACAAAGCGGGCCAGGGTTTCTGAAGGCTTAAGTTTGGCCTCTGCTGCCAAAACAGCTG
TTGCTGAGTATTATGCTTCAAACGGGTCATTCCCCTCAAATAACACCTCTGCTGGTTTGCAGGCATCAATTAGTGGCAAC
TCGGTAACTAGTGTGCAGGTTGGTACAAACGGTGTTATTACAGTTACCTATAGCTCCACATTGATTGATACTAGCAAGAA
TCAATTGACACTAACCCCTACTGCATCGGGTGGATCGATTGCCTGGACATGTGGAGCTTCGGGGACTACTGGACTCAATC
CTCAATGGCTACCCGCTAACTGCCGTGCCGGCAACTGA

Domains

Predicted by InterproScan.

• Pilin (bacterial filament) (Pilin) (37-142)

• Prokaryotic N-terminal methylation motif (N_methyl) (3-27)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Ralstonia pseudosolanacearum GMI1000	43.713	100	0.503
pilE	Neisseria gonorrhoeae MS11	44.375	100	0.49
pilA2	Legionella pneumophila str. Paris	51.079	95.862	0.49
pilA2	Legionella pneumophila strain ERS1305867	51.079	95.862	0.49
pilE	Neisseria gonorrhoeae strain FA1090	43.396	100	0.476
comP	Acinetobacter baylyi ADP1	45.946	100	0.469
pilA/pilA1	Eikenella corrodens VA1	39.241	100	0.428
pilA	Haemophilus influenzae 86-028NP	43.357	98.621	0.428
pilA	Haemophilus influenzae Rd KW20	41.667	99.31	0.414
pilA	Vibrio cholerae strain A1552	38.961	100	0.414
pilA	Vibrio cholerae C6706	38.961	100	0.414
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	38.961	100	0.414
pilA	Acinetobacter baumannii strain A118	40.411	100	0.407
pilA/pilAII	Pseudomonas stutzeri DSM 10701	39.726	100	0.4
pilA	Pseudomonas aeruginosa PAK	37.255	100	0.393
pilA	Vibrio parahaemolyticus RIMD 2210633	42.969	88.276	0.379
pilA/pilAI	Pseudomonas stutzeri DSM 10701	38.298	97.241	0.372