Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   DXT74_RS00710 Genome accession   NZ_CP031514
Coordinates   133086..133505 (-) Length   139 a.a.
NCBI ID   WP_194427090.1    Uniprot ID   -
Organism   Chromobacterium sp. Rain0013     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 128086..138505
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DXT74_RS00695 (DXT74_00700) - 128904..130688 (-) 1785 WP_194427087.1 PglL family O-oligosaccharyltransferase -
  DXT74_RS00700 (DXT74_00705) - 130764..132524 (-) 1761 WP_194427088.1 PglL family O-oligosaccharyltransferase -
  DXT74_RS00705 (DXT74_00710) pilA 132636..133073 (-) 438 WP_194427089.1 pilin Machinery gene
  DXT74_RS00710 (DXT74_00715) pilA2 133086..133505 (-) 420 WP_194427090.1 pilin Machinery gene
  DXT74_RS00715 (DXT74_00720) - 133702..135054 (+) 1353 WP_194427091.1 adenosylmethionine--8-amino-7-oxononanoate transaminase -
  DXT74_RS00720 (DXT74_00725) - 135156..136658 (+) 1503 WP_194427092.1 hypothetical protein -
  DXT74_RS00725 (DXT74_00730) proB 136858..137976 (+) 1119 WP_019100517.1 glutamate 5-kinase -

Sequence

Protein


Download         Length: 139 a.a.        Molecular weight: 14317.33 Da        Isoelectric Point: 6.9379

>NTDB_id=307237 DXT74_RS00710 WP_194427090.1 133086..133505(-) (pilA2) [Chromobacterium sp. Rain0013]
MRKEAGFTLIELMIVVAIIGILAAIAIPAYQNYVIRAKVAEGLTYADQAKTSVSEYYLAGNTWPSTNASAGLPNTLAGNY
VSNVQVGVSSGVSTVTVLFNSSVVSGLTVVFTPVASGGTVAWSCSTGAAYAQYVPSSCH

Nucleotide


Download         Length: 420 bp        

>NTDB_id=307237 DXT74_RS00710 WP_194427090.1 133086..133505(-) (pilA2) [Chromobacterium sp. Rain0013]
ATGAGGAAGGAGGCTGGTTTTACCTTGATTGAACTAATGATTGTTGTCGCTATTATCGGCATTTTGGCAGCGATTGCGAT
TCCAGCGTACCAGAACTATGTGATTCGAGCCAAAGTGGCGGAAGGCTTGACCTATGCGGATCAGGCCAAAACCTCGGTCT
CAGAATACTATCTGGCTGGCAATACTTGGCCAAGCACTAATGCTAGCGCGGGCTTGCCGAATACTTTGGCAGGAAACTAT
GTCAGTAATGTACAGGTTGGAGTTAGTAGTGGAGTCAGTACTGTGACCGTGTTGTTTAATAGCAGTGTGGTCTCTGGTTT
GACTGTGGTCTTTACCCCTGTAGCTAGTGGTGGTACTGTTGCTTGGTCGTGCTCAACCGGTGCGGCTTATGCTCAATATG
TGCCTTCGTCTTGTCATTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(36-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

50

99.281

0.496
  pilA2 Legionella pneumophila strain ERS1305867

49.275

99.281

0.489
  comP Acinetobacter baylyi ADP1

44.295

100

0.475
  pilE Neisseria gonorrhoeae MS11

38.217

100

0.432
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

31.351

100

0.417
  pilA Ralstonia pseudosolanacearum GMI1000

45.6

89.928

0.41
  pilE Neisseria gonorrhoeae strain FA1090

36.538

100

0.41
  pilA/pilA1 Eikenella corrodens VA1

37.333

100

0.403
  pilA Pseudomonas aeruginosa PAK

35.762

100

0.388
  pilA Vibrio cholerae strain A1552

36.552

100

0.381
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

36.552

100

0.381
  pilA Vibrio cholerae C6706

36.552

100

0.381
  pilA Vibrio parahaemolyticus RIMD 2210633

41.6

89.928

0.374
  pilA/pilAII Pseudomonas stutzeri DSM 10701

37.681

99.281

0.374

Multiple sequence alignment