Detailed information NTG0030645

Bioinformatically predicted

Overview

• Name: comGF/cglF
• Type: Machinery gene
• Locus tag: SPCG_RS10610
• Genome accession: NC_010582
• Coordinates: 1997972..1998433 (-)
• Length: 153 a.a.
• NCBI ID: WP_000250539.1
• Uniprot ID: -
• Organism: Streptococcus pneumoniae CGSP14
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1992972..2003433

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SPCG_RS10575 (SPCG_2007)	-	1993277..1994101 (-)	825	WP_000727904.1	membrane protein insertase YidC	-
SPCG_RS10580 (SPCG_2008)	rnpA	1994076..1994447 (-)	372	WP_000739246.1	ribonuclease P protein component	-
SPCG_RS14075 (SPCG_2009)	-	1994464..1994595 (-)	132	WP_000768904.1	hypothetical protein	-
SPCG_RS10585 (SPCG_2010)	-	1994596..1995786 (-)	1191	WP_000167766.1	acetate kinase	-
SPCG_RS10590 (SPCG_2011)	comYH	1995837..1996790 (-)	954	WP_000345133.1	class I SAM-dependent methyltransferase	Machinery gene
SPCG_RS12795	-	1996851..1997445 (-)	595	Protein_1978	class I SAM-dependent methyltransferase	-
SPCG_RS10605 (SPCG_2014)	comGG/cglG	1997548..1997994 (-)	447	WP_001811371.1	competence type IV pilus minor pilin ComGG	Machinery gene
SPCG_RS10610 (SPCG_2015)	comGF/cglF	1997972..1998433 (-)	462	WP_000250539.1	competence type IV pilus minor pilin ComGF	Machinery gene
SPCG_RS10615 (SPCG_2016)	comGE/cglE	1998396..1998698 (-)	303	WP_000413382.1	competence type IV pilus minor pilin ComGE	Machinery gene
SPCG_RS10620 (SPCG_2017)	comGD/cglD	1998661..1999065 (-)	405	WP_000588030.1	competence type IV pilus minor pilin ComGD	Machinery gene
SPCG_RS10625 (SPCG_2018)	comGC/cglC	1999058..1999384 (-)	327	WP_000738625.1	competence type IV pilus major pilin ComGC	Machinery gene
SPCG_RS10630 (SPCG_2019)	comGB/cglB	1999386..2000402 (-)	1017	WP_078134418.1	competence type IV pilus assembly protein ComGB	Machinery gene
SPCG_RS10635 (SPCG_2020)	comGA/cglA/cilD	2000350..2001291 (-)	942	WP_000249573.1	competence type IV pilus ATPase ComGA	Machinery gene
SPCG_RS10640 (SPCG_2021)	-	2001367..2001732 (-)	366	WP_000286415.1	DUF1033 family protein	-
SPCG_RS10645 (SPCG_2022)	-	2001883..2002941 (-)	1059	WP_000649468.1	zinc-dependent alcohol dehydrogenase family protein	-

Sequence

Protein

• Length: 153 a.a.
• Molecular weight: 17851.34 Da
• Isoelectric Point: 6.4832

>NTDB_id=30645 SPCG_RS10610 WP_000250539.1 1997972..1998433(-) (comGF/cglF) [Streptococcus pneumoniae CGSP14]
MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQTMSQLLISEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGN
RLYMKQDGKDIAIGKSKSDDFRKTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES

Nucleotide

• Length: 462 bp

>NTDB_id=30645 SPCG_RS10610 WP_000250539.1 1997972..1998433(-) (comGF/cglF) [Streptococcus pneumoniae CGSP14]
ATGGTTCAGAACAGTTGTTGGCAATCAAAGAGCCATAAGGTCAAGGCTTTTACCTTGTTAGAATCCCTGCTTGCCCTCAT
TGTCATCAGTGGGGGATTACTCCTTTTTCAAACTATGAGTCAGCTCCTCATTTCAGAAGTTCGCTACCAGCAACAAAGCG
AGCAAAAGGAGTGGCTCTTGTTTGTGGACCAACTTGAGGCAGAATTAGACCGTTCGCAGTTCGAAAAAGTAGAAGGCAAT
CGCCTATACATGAAGCAAGATGGCAAGGACATCGCCATCGGTAAGTCAAAGTCAGACGATTTTCGTAAAACCGATGCTAG
TGGACGGGGGTATCAGCCTATGGTTTATGGCCTCAAATCAGCTCAGATTACAGAGGACAATCAACTGGTTCATTTTCGTT
TCCAGTTTCAAAAAGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGAAGAAAGTTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (45-141)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF/cglF	Streptococcus pneumoniae Rx1	94.118	100	0.941
comGF/cglF	Streptococcus pneumoniae D39	94.118	100	0.941
comGF/cglF	Streptococcus pneumoniae R6	94.118	100	0.941
comGF/cglF	Streptococcus pneumoniae TIGR4	94.118	100	0.941
comGF/cglF	Streptococcus mitis SK321	92.81	100	0.928
comGF/cglF	Streptococcus mitis NCTC 12261	91.503	100	0.915