Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SALB1_RS17885 Genome accession   NZ_CP029488
Coordinates   3985266..3986300 (-) Length   344 a.a.
NCBI ID   WP_109995085.1    Uniprot ID   A0A2Z3J9J9
Organism   Salinisphaera sp. LB1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3980266..3991300
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SALB1_RS17865 (SALB1_3628) rimK 3981466..3982371 (-) 906 WP_109995081.1 30S ribosomal protein S6--L-glutamate ligase -
  SALB1_RS17870 (SALB1_3629) - 3982479..3982937 (-) 459 WP_109995082.1 RimK/LysX family protein -
  SALB1_RS17875 (SALB1_3630) - 3982975..3984057 (-) 1083 WP_109995083.1 PilT/PilU family type 4a pilus ATPase -
  SALB1_RS17880 (SALB1_3631) pilU 3984047..3985231 (-) 1185 WP_109995084.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SALB1_RS17885 (SALB1_3632) pilT 3985266..3986300 (-) 1035 WP_109995085.1 type IV pilus twitching motility protein PilT Machinery gene
  SALB1_RS17890 (SALB1_3633) - 3986404..3987123 (+) 720 WP_109995086.1 YggS family pyridoxal phosphate-dependent enzyme -
  SALB1_RS17895 (SALB1_3634) rpiA 3987202..3987864 (+) 663 WP_109995087.1 ribose-5-phosphate isomerase RpiA -
  SALB1_RS17900 (SALB1_3635) - 3987935..3989116 (+) 1182 WP_255414451.1 MFS transporter -
  SALB1_RS17905 (SALB1_3636) - 3989214..3989924 (+) 711 WP_109995088.1 molybdenum cofactor biosynthesis protein MoaE -
  SALB1_RS17910 (SALB1_3637) moaCB 3989979..3990896 (+) 918 WP_109995089.1 bifunctional molybdenum cofactor biosynthesis protein MoaC/MoaB -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38022.51 Da        Isoelectric Point: 6.2450

>NTDB_id=293135 SALB1_RS17885 WP_109995085.1 3985266..3986300(-) (pilT) [Salinisphaera sp. LB1]
MDISQLLAFSVKQGASDLHLSAGLPPMIRVDGDIRRINLPSMEDKEVFDLIYDIMTDRQRRDFDEHLETDFSFEISGLAR
FRVNAYNQNRGAAAVFRTIPADVLSLEDLEAPPIFREIASLPRGLVLVTGPTGSGKSTTLAAMVNHKNDTELGHILTIED
PIEFVHSSRKCLVNQREISRDTHSFSAALRSALREDPDTILVGELRDLETIRLALTAAETGHLVLGTLHTSSAAKTIDRI
IDVFPGEEKEMVRAMLSESLRAVISQALLKKKPGGRLAAHEIMMGTPAIRNLIREGKTAQMYSAIQTGQQAGMQTLDQCL
QRLVQRGMVDREEAARKAANKDSI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=293135 SALB1_RS17885 WP_109995085.1 3985266..3986300(-) (pilT) [Salinisphaera sp. LB1]
ATGGATATTTCGCAGCTGCTGGCGTTCTCGGTCAAACAGGGCGCCTCCGATCTGCACCTGTCGGCGGGGCTGCCGCCGAT
GATTCGCGTGGATGGCGATATCCGCCGCATCAACCTGCCGTCGATGGAAGACAAGGAAGTCTTCGATCTCATCTACGACA
TCATGACCGATCGCCAGCGGCGTGATTTCGACGAGCATCTGGAAACGGACTTCTCGTTCGAGATCAGCGGCCTGGCGCGC
TTTCGCGTCAACGCCTACAATCAGAATCGCGGCGCGGCCGCGGTGTTTCGTACCATTCCCGCGGACGTGCTGTCGCTGGA
GGATCTGGAAGCGCCGCCGATCTTTCGCGAGATCGCGTCGTTGCCGCGCGGGCTGGTGCTGGTCACCGGGCCGACCGGCT
CCGGCAAGTCGACCACGCTGGCGGCGATGGTCAATCACAAGAACGACACCGAGCTTGGGCATATCCTCACCATCGAGGAT
CCGATCGAATTCGTGCACAGCTCGCGCAAGTGCCTGGTCAATCAGCGCGAAATCTCGCGCGATACGCACTCCTTCAGCGC
AGCACTCAGATCCGCGCTGCGTGAGGATCCGGATACCATCCTGGTCGGCGAGCTGCGCGATCTGGAAACCATCCGGCTGG
CCTTGACCGCGGCCGAAACCGGGCATCTGGTGCTGGGTACGCTGCATACCTCGTCCGCGGCCAAGACCATCGACCGCATC
ATCGATGTGTTCCCCGGCGAGGAGAAGGAAATGGTGCGCGCGATGCTGTCGGAGTCGTTGCGCGCGGTGATTTCGCAGGC
GCTGCTCAAGAAAAAGCCGGGCGGCCGGCTGGCCGCGCACGAGATCATGATGGGCACGCCGGCGATTCGTAACCTGATTC
GCGAGGGCAAGACCGCGCAGATGTATTCGGCGATCCAGACCGGCCAGCAGGCGGGCATGCAGACACTCGATCAATGCCTG
CAGCGACTGGTTCAGCGCGGTATGGTCGATCGCGAGGAGGCCGCCCGCAAGGCGGCCAACAAGGATTCGATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2Z3J9J9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

74.128

100

0.741
  pilT Pseudomonas aeruginosa PAK

75.148

98.256

0.738
  pilT Acinetobacter baumannii D1279779

73.607

99.128

0.73
  pilT Acinetobacter baumannii strain A118

73.607

99.128

0.73
  pilT Acinetobacter nosocomialis M2

73.607

99.128

0.73
  pilT Acinetobacter baylyi ADP1

71.802

100

0.718
  pilT Legionella pneumophila strain Lp02

72.141

99.128

0.715
  pilT Legionella pneumophila strain ERS1305867

72.141

99.128

0.715
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.249

97.965

0.669
  pilT Vibrio cholerae strain A1552

68.249

97.965

0.669
  pilT Neisseria meningitidis 8013

65.797

100

0.66
  pilT Neisseria gonorrhoeae MS11

65.507

100

0.657
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.147

98.547

0.494
  pilU Pseudomonas stutzeri DSM 10701

41.194

97.384

0.401
  pilU Vibrio cholerae strain A1552

39.42

100

0.395
  pilU Acinetobacter baylyi ADP1

38.728

100

0.39

Multiple sequence alignment