Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   SALB1_RS17880 Genome accession   NZ_CP029488
Coordinates   3984047..3985231 (-) Length   394 a.a.
NCBI ID   WP_109995084.1    Uniprot ID   A0A2Z3JJW0
Organism   Salinisphaera sp. LB1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3979047..3990231
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SALB1_RS17855 (SALB1_3626) - 3979316..3980293 (+) 978 WP_109995079.1 aspartate carbamoyltransferase catalytic subunit -
  SALB1_RS17860 (SALB1_3627) - 3980260..3981339 (-) 1080 WP_109995080.1 succinylglutamate desuccinylase/aspartoacylase family protein -
  SALB1_RS17865 (SALB1_3628) rimK 3981466..3982371 (-) 906 WP_109995081.1 30S ribosomal protein S6--L-glutamate ligase -
  SALB1_RS17870 (SALB1_3629) - 3982479..3982937 (-) 459 WP_109995082.1 RimK/LysX family protein -
  SALB1_RS17875 (SALB1_3630) - 3982975..3984057 (-) 1083 WP_109995083.1 PilT/PilU family type 4a pilus ATPase -
  SALB1_RS17880 (SALB1_3631) pilU 3984047..3985231 (-) 1185 WP_109995084.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SALB1_RS17885 (SALB1_3632) pilT 3985266..3986300 (-) 1035 WP_109995085.1 type IV pilus twitching motility protein PilT Machinery gene
  SALB1_RS17890 (SALB1_3633) - 3986404..3987123 (+) 720 WP_109995086.1 YggS family pyridoxal phosphate-dependent enzyme -
  SALB1_RS17895 (SALB1_3634) rpiA 3987202..3987864 (+) 663 WP_109995087.1 ribose-5-phosphate isomerase RpiA -
  SALB1_RS17900 (SALB1_3635) - 3987935..3989116 (+) 1182 WP_255414451.1 MFS transporter -
  SALB1_RS17905 (SALB1_3636) - 3989214..3989924 (+) 711 WP_109995088.1 molybdenum cofactor biosynthesis protein MoaE -

Sequence

Protein


Download         Length: 394 a.a.        Molecular weight: 43686.82 Da        Isoelectric Point: 6.1931

>NTDB_id=293134 SALB1_RS17880 WP_109995084.1 3984047..3985231(-) (pilU) [Salinisphaera sp. LB1]
MDRAQATKLVTVLLERLIADGGSDLFITAGFAPAIKKDGQLTPLMERPLTEEQSALMVRSLMNDRQAREFDEFKEAQFAI
SPSSGRFRVSAFVQQGRVGAVLRVINTEIPEFDDLGLPPILKDVVMAERGLVIFVGATGSGKSTSLAAMLGHRNANQAGH
IITIEDPIEFVHRHRKSIVTQREVGVDTQSWENALKNTLRQAPNVILVGEVRTRETMEYALNFAETGHLVLCTLHANNAN
QALDRVINFFDEGKREQVLMDLSLNMRAIISQRLVRKVGGGRAAAVEVLLNTPLVTDLVFKGKVAELRDVMARGTELGMQ
TFDQHLFQLFEDGLIDYAEALRHADSQNELRLNIKLNSTRARQNLSMDPEVRSLTMAEHEDGARRGDSGDPHGQ

Nucleotide


Download         Length: 1185 bp        

>NTDB_id=293134 SALB1_RS17880 WP_109995084.1 3984047..3985231(-) (pilU) [Salinisphaera sp. LB1]
ATGGATCGCGCCCAGGCCACCAAACTCGTGACCGTGTTGCTGGAACGGCTGATTGCCGACGGCGGCTCGGATCTGTTCAT
CACCGCCGGCTTCGCGCCCGCAATCAAGAAGGACGGCCAGCTCACGCCCCTCATGGAGCGTCCGCTCACCGAGGAACAAT
CGGCGCTGATGGTGCGATCGCTCATGAACGATCGGCAGGCGCGCGAGTTCGACGAGTTCAAGGAGGCGCAGTTCGCGATC
TCGCCGTCGTCAGGGCGTTTTCGCGTTTCGGCGTTCGTGCAGCAGGGCCGGGTGGGCGCGGTGCTCCGCGTGATCAACAC
CGAAATCCCCGAGTTCGACGATCTGGGCCTGCCGCCGATCCTCAAGGATGTGGTCATGGCCGAGCGCGGCCTGGTGATCT
TCGTCGGCGCGACCGGCTCGGGCAAATCGACCTCGCTCGCGGCCATGCTCGGCCATCGCAATGCCAATCAGGCCGGCCAC
ATCATCACCATCGAGGACCCGATCGAGTTCGTGCACCGGCATCGCAAGTCCATCGTGACCCAGCGCGAGGTCGGCGTGGA
CACCCAGTCCTGGGAGAACGCGCTCAAGAATACGCTGCGTCAGGCGCCCAACGTGATCCTGGTGGGCGAGGTGCGCACGC
GCGAGACCATGGAATACGCGCTCAACTTCGCCGAGACCGGGCATCTGGTGCTGTGCACGCTGCATGCGAACAACGCCAAC
CAGGCGCTCGACCGAGTGATCAATTTCTTCGACGAGGGCAAGCGCGAACAGGTGCTCATGGATCTGTCGCTCAACATGCG
CGCAATCATTTCCCAGCGTCTGGTGCGCAAGGTCGGCGGCGGTCGTGCGGCGGCGGTCGAGGTGTTGCTCAACACGCCGC
TGGTGACCGACCTGGTCTTCAAGGGCAAGGTCGCCGAACTGCGCGATGTCATGGCGCGCGGCACGGAGTTGGGCATGCAG
ACCTTCGATCAGCACTTGTTCCAGCTGTTCGAAGACGGGTTGATCGACTACGCCGAGGCGCTGCGCCACGCCGATTCGCA
GAACGAACTGCGCCTGAACATCAAGCTCAACAGCACCCGGGCGCGCCAGAACCTGTCGATGGACCCGGAGGTGCGCTCGC
TGACCATGGCCGAGCACGAAGACGGCGCACGGCGCGGCGACTCCGGGGATCCGCATGGACAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2Z3JJW0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

57.796

94.416

0.546
  pilU Acinetobacter baylyi ADP1

54.188

96.954

0.525
  pilU Vibrio cholerae strain A1552

51.791

92.132

0.477
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.953

96.193

0.404
  pilT Vibrio cholerae strain A1552

42.442

87.31

0.371
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.442

87.31

0.371
  pilT Acinetobacter nosocomialis M2

42.815

86.548

0.371
  pilT Acinetobacter baumannii D1279779

42.815

86.548

0.371
  pilT Acinetobacter baumannii strain A118

42.815

86.548

0.371
  pilT Pseudomonas aeruginosa PAK

43.373

84.264

0.365

Multiple sequence alignment