Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PING_RS15655 Genome accession   NC_008709
Coordinates   3738841..3739875 (-) Length   344 a.a.
NCBI ID   WP_011771295.1    Uniprot ID   A1SZ26
Organism   Psychromonas ingrahamii 37     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3733841..3744875
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PING_RS15640 (Ping_3036) srmB 3735448..3736809 (+) 1362 WP_011771292.1 ATP-dependent RNA helicase SrmB -
  PING_RS15645 (Ping_3037) yaaA 3736861..3737634 (-) 774 WP_011771293.1 peroxide stress protein YaaA -
  PING_RS15650 (Ping_3038) pilU 3737713..3738828 (-) 1116 WP_011771294.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PING_RS15655 (Ping_3039) pilT 3738841..3739875 (-) 1035 WP_011771295.1 type IV pilus twitching motility protein PilT Machinery gene
  PING_RS15660 (Ping_3040) - 3739894..3740592 (+) 699 WP_011771296.1 YggS family pyridoxal phosphate-dependent enzyme -
  PING_RS15665 (Ping_3041) proC 3740848..3741672 (+) 825 WP_011771297.1 pyrroline-5-carboxylate reductase -
  PING_RS15670 (Ping_3042) - 3741704..3742246 (+) 543 WP_011771298.1 YggT family protein -
  PING_RS15675 (Ping_3043) yggU 3742246..3742542 (+) 297 WP_011771299.1 DUF167 family protein YggU -
  PING_RS15680 (Ping_3044) - 3742561..3743001 (+) 441 WP_011771300.1 DUF4426 domain-containing protein -
  PING_RS15685 (Ping_3045) - 3743034..3743636 (+) 603 WP_011771301.1 XTP/dITP diphosphatase -
  PING_RS15690 (Ping_3046) hemW 3743707..3744849 (+) 1143 WP_011771302.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38113.88 Da        Isoelectric Point: 6.7324

>NTDB_id=27116 PING_RS15655 WP_011771295.1 3738841..3739875(-) (pilT) [Psychromonas ingrahamii 37]
MDITELLAFSVKHNASDLHLSSGVPPMIRVDGEVRKLNVPALDPKEAQTLIYDIMNDKQRKDFEEDLETDFSFEIPGLAR
FRVNAFNQNRGVSAVFRTIPSKVLTLDELGAPEIFKTISEYPRGLVLVTGPTGSGKSTTLAAMIDYINESRHEHILTIED
PVEFVHETKKSLINQREVYRHTLSFQRALKSALREDPDIILVGELRDLETIRLAITAAETGHLVFATLHTTSAAKTINRI
IDVFPAGEKSMVRSMLSESLRAVISQALLKKPGGGRVAAHEIMLGIPAIRNLIREDKVAQMYSVIQTGMVHGMQTLDQSL
RDLVGQGLIAHADATKKAVDPKTF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=27116 PING_RS15655 WP_011771295.1 3738841..3739875(-) (pilT) [Psychromonas ingrahamii 37]
ATGGATATAACAGAATTATTGGCTTTCAGTGTAAAGCATAATGCGTCAGATCTACACCTTTCTTCAGGAGTTCCTCCAAT
GATTAGGGTCGATGGGGAGGTGCGTAAATTAAATGTCCCTGCTTTAGATCCTAAAGAGGCACAGACTCTTATTTACGACA
TTATGAATGATAAACAGCGTAAAGATTTTGAAGAGGATTTAGAAACCGACTTTTCCTTTGAAATTCCAGGCTTAGCGCGT
TTCCGTGTTAATGCTTTTAACCAGAACCGTGGTGTATCGGCTGTCTTTCGTACTATCCCAAGCAAAGTGTTAACATTAGA
TGAACTGGGCGCACCTGAAATATTTAAAACAATATCAGAATACCCTCGCGGTTTAGTCTTAGTAACGGGGCCAACGGGCT
CGGGTAAGTCAACCACCTTAGCAGCAATGATTGACTATATTAATGAAAGTCGTCATGAGCACATTTTAACCATCGAAGAT
CCTGTCGAATTTGTCCATGAAACAAAAAAATCGCTGATCAACCAGCGTGAAGTTTATAGACACACCTTAAGTTTTCAGCG
CGCACTAAAAAGTGCATTGCGGGAAGATCCCGATATTATTTTAGTCGGAGAACTGCGTGATTTAGAAACCATTCGTTTAG
CAATCACAGCAGCGGAAACGGGTCACTTGGTCTTTGCTACCCTGCATACCACTTCTGCGGCTAAAACGATTAACCGTATT
ATTGATGTCTTTCCTGCGGGAGAGAAAAGCATGGTGCGCTCAATGTTATCTGAATCATTACGTGCCGTTATTTCGCAAGC
TTTACTTAAAAAACCGGGTGGCGGGCGAGTTGCTGCGCATGAAATTATGTTGGGCATTCCCGCAATTCGGAATTTGATTC
GTGAAGATAAAGTTGCGCAAATGTACTCAGTAATTCAAACCGGAATGGTACATGGCATGCAAACACTAGATCAAAGTTTA
CGTGATTTAGTTGGCCAGGGTCTTATTGCACACGCTGATGCTACGAAAAAAGCGGTCGATCCAAAAACATTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A1SZ26

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

77.035

100

0.77
  pilT Pseudomonas stutzeri DSM 10701

76.744

100

0.767
  pilT Acinetobacter baumannii strain A118

76.453

100

0.765
  pilT Acinetobacter nosocomialis M2

76.453

100

0.765
  pilT Acinetobacter baumannii D1279779

76.453

100

0.765
  pilT Pseudomonas aeruginosa PAK

76.163

100

0.762
  pilT Legionella pneumophila strain Lp02

71.512

100

0.715
  pilT Legionella pneumophila strain ERS1305867

71.512

100

0.715
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.855

100

0.701
  pilT Vibrio cholerae strain A1552

69.855

100

0.701
  pilT Neisseria gonorrhoeae MS11

67.246

100

0.674
  pilT Neisseria meningitidis 8013

67.246

100

0.674
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

100

0.515
  pilU Acinetobacter baylyi ADP1

41.04

100

0.413
  pilU Vibrio cholerae strain A1552

41.593

98.547

0.41
  pilU Pseudomonas stutzeri DSM 10701

40.597

97.384

0.395

Multiple sequence alignment