Detailed information NTG0027115

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PING_RS15650
• Genome accession: NC_008709
• Coordinates: 3737713..3738828 (-)
• Length: 371 a.a.
• NCBI ID: WP_011771294.1
• Uniprot ID: A1SZ25
• Organism: Psychromonas ingrahamii 37
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3732713..3743828

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PING_RS15635 (Ping_3035)	glgA	3733714..3735180 (+)	1467	WP_011771291.1	glycogen synthase GlgA	-
PING_RS15640 (Ping_3036)	srmB	3735448..3736809 (+)	1362	WP_011771292.1	ATP-dependent RNA helicase SrmB	-
PING_RS15645 (Ping_3037)	yaaA	3736861..3737634 (-)	774	WP_011771293.1	peroxide stress protein YaaA	-
PING_RS15650 (Ping_3038)	pilU	3737713..3738828 (-)	1116	WP_011771294.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
PING_RS15655 (Ping_3039)	pilT	3738841..3739875 (-)	1035	WP_011771295.1	type IV pilus twitching motility protein PilT	Machinery gene
PING_RS15660 (Ping_3040)	-	3739894..3740592 (+)	699	WP_011771296.1	YggS family pyridoxal phosphate-dependent enzyme	-
PING_RS15665 (Ping_3041)	proC	3740848..3741672 (+)	825	WP_011771297.1	pyrroline-5-carboxylate reductase	-
PING_RS15670 (Ping_3042)	-	3741704..3742246 (+)	543	WP_011771298.1	YggT family protein	-
PING_RS15675 (Ping_3043)	yggU	3742246..3742542 (+)	297	WP_011771299.1	DUF167 family protein YggU	-
PING_RS15680 (Ping_3044)	-	3742561..3743001 (+)	441	WP_011771300.1	DUF4426 domain-containing protein	-
PING_RS15685 (Ping_3045)	-	3743034..3743636 (+)	603	WP_011771301.1	XTP/dITP diphosphatase	-

Sequence

Protein

• Length: 371 a.a.
• Molecular weight: 41557.64 Da
• Isoelectric Point: 6.1909

>NTDB_id=27115 PING_RS15650 WP_011771294.1 3737713..3738828(-) (pilU) [Psychromonas ingrahamii 37]
MLEKLLIKVDESKASDLYISVGLPATMKLNGTLKAINEHILTSEEVYDLLKEVMDEKHFNEFMTIKESNFAHHPNDGKRF
RISAFMQKGQPAMVLRRIETKIPEFAEINLPERLKDVCLAKEGLILFVGATGVGKSTTQAAMIGFRNQNSCDHILTIEDP
IEFIHEHGKSVITQREIGVDTGSFDEALKNSLRQAADVIVVGEIRTRQTMEFVLSFAETGHLCMATMHAKNANQALDRIL
HLVPQENHRQFLFDLSVNLRAIVAQQLIPTTDGQGRRAAFEILYNTPTMAEAIRTGELHTLKQIMKSSGQHGMQTFDQAL
FDLYESGIIGYTEALAYSDSANDVRLMIKLKSTESRKSLDSGKLSDVTVDF

Nucleotide

• Length: 1116 bp

>NTDB_id=27115 PING_RS15650 WP_011771294.1 3737713..3738828(-) (pilU) [Psychromonas ingrahamii 37]
ATGTTAGAAAAGTTATTAATTAAGGTCGATGAATCAAAAGCATCTGATTTATATATTTCAGTTGGTTTGCCGGCAACAAT
GAAATTAAATGGAACATTAAAGGCTATTAATGAACATATACTGACCAGCGAAGAAGTTTATGATCTCTTAAAAGAGGTCA
TGGATGAAAAACATTTTAATGAATTTATGACTATTAAAGAATCCAATTTTGCGCACCATCCCAACGATGGTAAGCGTTTT
AGGATCAGTGCTTTTATGCAAAAAGGGCAACCTGCTATGGTGCTCCGCCGTATAGAAACTAAAATACCTGAATTTGCTGA
GATAAATTTACCGGAACGATTAAAAGATGTCTGTCTGGCAAAAGAGGGCTTAATTTTATTTGTAGGTGCAACGGGTGTCG
GTAAATCAACGACACAAGCAGCAATGATTGGTTTTCGAAATCAAAATTCATGCGATCATATCCTGACCATTGAAGATCCG
ATTGAGTTTATTCACGAACATGGAAAATCTGTGATCACCCAACGCGAAATTGGTGTTGATACTGGTTCATTTGATGAGGC
GCTGAAAAATTCATTACGCCAAGCGGCTGATGTTATTGTCGTCGGTGAAATACGAACACGACAAACCATGGAATTCGTTC
TGTCATTTGCTGAGACCGGGCATTTATGTATGGCGACTATGCATGCTAAAAATGCGAATCAGGCTTTAGATCGTATTCTA
CATTTAGTGCCGCAGGAAAATCATCGCCAGTTTCTATTTGATCTGTCGGTCAATTTACGTGCAATAGTTGCCCAGCAGTT
AATTCCAACAACAGATGGACAAGGGCGGCGAGCTGCATTTGAAATTTTATATAATACACCGACCATGGCTGAAGCTATTC
GTACTGGCGAGCTGCATACCCTTAAACAGATTATGAAATCATCAGGACAACATGGTATGCAAACCTTTGACCAAGCGCTG
TTTGATTTATATGAAAGCGGCATTATCGGTTATACCGAAGCATTGGCCTATTCTGATTCAGCAAATGATGTACGCTTAAT
GATTAAGCTTAAATCAACTGAAAGCCGAAAAAGTTTAGACAGTGGCAAGCTTTCTGATGTTACAGTCGACTTTTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (74-269)

3D structure

• AlphaFold DB: A1SZ25

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	55	97.035	0.534
pilU	Vibrio cholerae strain A1552	54.875	96.765	0.531
pilU	Acinetobacter baylyi ADP1	52.857	94.34	0.499
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.925	86.792	0.364