Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SDE_RS19015 Genome accession   NC_007912
Coordinates   4617304..4618338 (-) Length   344 a.a.
NCBI ID   WP_011470110.1    Uniprot ID   Q21EI4
Organism   Saccharophagus degradans 2-40     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4612304..4623338
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SDE_RS18985 (Sde_3634) pilG 4612404..4612799 (-) 396 WP_011470104.1 twitching motility response regulator PilG Regulator
  SDE_RS18990 (Sde_3635) gshB 4613090..4614013 (+) 924 WP_041324902.1 glutathione synthase -
  SDE_RS18995 (Sde_3636) - 4614033..4614917 (+) 885 WP_011470106.1 energy transducer TonB -
  SDE_RS19000 (Sde_3637) - 4615006..4615617 (+) 612 WP_011470107.1 YqgE/AlgH family protein -
  SDE_RS19005 (Sde_3638) ruvX 4615675..4616103 (+) 429 WP_011470108.1 Holliday junction resolvase RuvX -
  SDE_RS19010 (Sde_3639) pilU 4616127..4617269 (-) 1143 WP_011470109.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SDE_RS19015 (Sde_3640) pilT 4617304..4618338 (-) 1035 WP_011470110.1 type IV pilus twitching motility protein PilT Machinery gene
  SDE_RS19020 (Sde_3641) - 4618515..4619195 (+) 681 WP_011470111.1 YggS family pyridoxal phosphate-dependent enzyme -
  SDE_RS19025 (Sde_3642) proC 4619235..4620092 (+) 858 WP_011470112.1 pyrroline-5-carboxylate reductase -
  SDE_RS19030 (Sde_3643) - 4620101..4620691 (+) 591 WP_011470113.1 YggT family protein -
  SDE_RS19035 (Sde_3644) metX 4620725..4621870 (+) 1146 WP_011470114.1 homoserine O-succinyltransferase MetX -
  SDE_RS19040 (Sde_3645) metW 4621872..4622468 (+) 597 WP_011470115.1 methionine biosynthesis protein MetW -
  SDE_RS19045 (Sde_3646) - 4622461..4622901 (+) 441 WP_011470116.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38154.75 Da        Isoelectric Point: 6.5785

>NTDB_id=25580 SDE_RS19015 WP_011470110.1 4617304..4618338(-) (pilT) [Saccharophagus degradans 2-40]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPMEHKQVHGLIYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGSGAVFRTIPSKVLTMEELGMGQVFRDVSSVPRGLVLVTGPTGSGKSTTLAAMIDYINDNKYHHILTIED
PIEFVHESKKCLVNQREVHRDTHGFAEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPANEKAMVRSMLSESLEAVVSQTLLKKNGGGRVAAHEIMRGTSAIRNLIREDKVAQMYSAIQTGSAVGMQTMDQCL
ADLVERRIISRDVAKEKAKMPDQF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=25580 SDE_RS19015 WP_011470110.1 4617304..4618338(-) (pilT) [Saccharophagus degradans 2-40]
ATGGATATTACTGAACTCCTCGCTTTCTCTGCCAAGCAGGGAGCATCGGATTTACACCTTTCGGCGGGTTTGCCGCCCAT
GATTCGTGTGGATGGCGATGTACGCCGCATTAACTTGCCGCCGATGGAGCACAAGCAAGTCCACGGGCTAATTTACGACA
TTATGAACGACAAGCAGCGCAAGGATTTCGAAGAATTCCTCGAGACTGACTTTTCGTTTGAAGTGCCCGGTGTGGCGCGC
TTCCGTGTTAACGCCTTTAACCAAAACCGCGGCTCTGGTGCGGTGTTTCGTACCATTCCTTCCAAAGTGCTTACCATGGA
AGAATTGGGTATGGGGCAAGTGTTCCGCGATGTATCTTCGGTGCCGCGCGGCTTGGTGTTGGTAACCGGGCCGACCGGTT
CGGGTAAGTCCACCACCCTTGCGGCCATGATCGATTATATTAACGACAACAAATACCACCATATTCTTACCATCGAAGAC
CCGATCGAATTCGTGCACGAAAGTAAGAAGTGCTTGGTAAACCAGCGTGAGGTGCACCGCGATACCCACGGCTTTGCTGA
AGCACTGCGCTCGGCACTGCGGGAAGACCCCGATATTATTCTGGTGGGTGAGCTGCGTGACTTGGAAACCATTCGCTTGG
CACTAACCGCGGCGGAAACCGGTCACTTGGTGTTCGGCACCTTGCACACCACCTCGGCGGCCAAAACCATTGACCGGGTA
GTGGATGTATTCCCCGCTAACGAGAAAGCTATGGTGCGTTCTATGTTGTCGGAATCCCTCGAAGCGGTTGTTTCGCAAAC
ACTTTTGAAGAAAAACGGCGGTGGCCGTGTAGCAGCGCACGAAATTATGCGCGGCACCTCAGCAATTCGTAACTTAATCC
GCGAAGATAAGGTTGCGCAAATGTATTCTGCTATTCAAACCGGTAGTGCGGTGGGCATGCAAACAATGGATCAGTGTTTG
GCCGATCTTGTTGAGCGCCGCATAATTAGTCGCGATGTAGCGAAAGAAAAAGCAAAAATGCCCGATCAGTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q21EI4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

88.663

100

0.887
  pilT Pseudomonas stutzeri DSM 10701

87.791

100

0.878
  pilT Acinetobacter nosocomialis M2

81.977

100

0.82
  pilT Acinetobacter baumannii D1279779

81.977

100

0.82
  pilT Acinetobacter baumannii strain A118

81.977

100

0.82
  pilT Acinetobacter baylyi ADP1

78.779

100

0.788
  pilT Legionella pneumophila strain Lp02

75.444

98.256

0.741
  pilT Legionella pneumophila strain ERS1305867

75.444

98.256

0.741
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.353

98.837

0.666
  pilT Vibrio cholerae strain A1552

67.353

98.837

0.666
  pilT Neisseria meningitidis 8013

67.353

98.837

0.666
  pilT Neisseria gonorrhoeae MS11

66.765

98.837

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.043

100

0.532
  pilU Vibrio cholerae strain A1552

41.231

94.477

0.39
  pilU Pseudomonas stutzeri DSM 10701

41.358

94.186

0.39
  pilU Acinetobacter baylyi ADP1

37.861

100

0.381

Multiple sequence alignment