Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   SDE_RS19010 Genome accession   NC_007912
Coordinates   4616127..4617269 (-) Length   380 a.a.
NCBI ID   WP_011470109.1    Uniprot ID   Q21EI5
Organism   Saccharophagus degradans 2-40     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4611127..4622269
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SDE_RS18975 (Sde_3632) - 4611398..4611937 (-) 540 WP_011470102.1 chemotaxis protein CheW -
  SDE_RS18980 (Sde_3633) pilH 4611988..4612350 (-) 363 WP_011470103.1 twitching motility response regulator PilH -
  SDE_RS18985 (Sde_3634) pilG 4612404..4612799 (-) 396 WP_011470104.1 twitching motility response regulator PilG Regulator
  SDE_RS18990 (Sde_3635) gshB 4613090..4614013 (+) 924 WP_041324902.1 glutathione synthase -
  SDE_RS18995 (Sde_3636) - 4614033..4614917 (+) 885 WP_011470106.1 energy transducer TonB -
  SDE_RS19000 (Sde_3637) - 4615006..4615617 (+) 612 WP_011470107.1 YqgE/AlgH family protein -
  SDE_RS19005 (Sde_3638) ruvX 4615675..4616103 (+) 429 WP_011470108.1 Holliday junction resolvase RuvX -
  SDE_RS19010 (Sde_3639) pilU 4616127..4617269 (-) 1143 WP_011470109.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SDE_RS19015 (Sde_3640) pilT 4617304..4618338 (-) 1035 WP_011470110.1 type IV pilus twitching motility protein PilT Machinery gene
  SDE_RS19020 (Sde_3641) - 4618515..4619195 (+) 681 WP_011470111.1 YggS family pyridoxal phosphate-dependent enzyme -
  SDE_RS19025 (Sde_3642) proC 4619235..4620092 (+) 858 WP_011470112.1 pyrroline-5-carboxylate reductase -
  SDE_RS19030 (Sde_3643) - 4620101..4620691 (+) 591 WP_011470113.1 YggT family protein -
  SDE_RS19035 (Sde_3644) metX 4620725..4621870 (+) 1146 WP_011470114.1 homoserine O-succinyltransferase MetX -

Sequence

Protein


Download         Length: 380 a.a.        Molecular weight: 42469.57 Da        Isoelectric Point: 6.0959

>NTDB_id=25579 SDE_RS19010 WP_011470109.1 4616127..4617269(-) (pilU) [Saccharophagus degradans 2-40]
MELDRLLSLMVDKQASDLFITAGVAPSIKLHGKIVPVSTTPLSPEKSRELVLSVMNEKQRKEFLEAKELNFAVSARGVGR
FRASAFYQRNLAGMVLRRIETNIPQIDELGLPEIIKELAMIKRGLVMFVGATGTGKSTSLAAMIGHRNRNSKGHIISIED
PIEFIHQHQGCIVTQREVGIDTDSFEVALKNTLRQAPDVILIGEVRSRETMDHAIAFAETGHLCLCTLHANNANQALDRI
IHFFPADRHRQLWMDLSLNLKGIVAQQLIPTPDGQGRRACLEVFLNTPLAADLVRKGEVHELKELMKRSTEQGMQTFDQA
LYELYDSGEITYEDALSHADSPNDLRLMIKLGSESDADYLSNAADELSIQEDESNRSRRF

Nucleotide


Download         Length: 1143 bp        

>NTDB_id=25579 SDE_RS19010 WP_011470109.1 4616127..4617269(-) (pilU) [Saccharophagus degradans 2-40]
ATGGAATTAGATAGACTGCTGAGTTTAATGGTAGATAAACAGGCTTCGGATCTGTTTATTACGGCTGGGGTGGCGCCTTC
CATTAAGCTGCACGGTAAAATTGTTCCGGTAAGTACCACGCCGCTTTCGCCAGAAAAGTCGCGCGAGCTGGTACTTAGTG
TAATGAATGAAAAACAGCGCAAAGAGTTCTTAGAAGCCAAAGAGCTTAACTTTGCGGTAAGTGCGCGCGGTGTGGGTCGA
TTCCGTGCCAGCGCCTTTTACCAGCGTAACCTGGCGGGTATGGTGTTGCGTCGTATTGAAACCAATATTCCGCAAATTGA
TGAGTTGGGCCTACCCGAAATAATCAAAGAGCTGGCGATGATTAAGCGCGGCTTGGTGATGTTTGTGGGGGCAACCGGTA
CCGGTAAATCCACCTCGTTAGCCGCGATGATTGGCCACCGCAATCGCAACTCCAAGGGCCATATTATTTCGATAGAAGAC
CCGATTGAATTTATCCACCAGCATCAGGGATGTATTGTTACCCAGCGCGAAGTGGGTATCGATACCGATTCTTTCGAGGT
AGCGTTAAAAAATACCCTGCGGCAGGCACCCGATGTTATTCTGATTGGTGAGGTTCGCTCGCGCGAAACCATGGACCACG
CAATCGCCTTCGCCGAAACGGGTCACCTGTGTTTATGTACGTTGCACGCTAACAACGCTAACCAAGCGTTGGATCGTATT
ATTCACTTCTTCCCCGCCGATCGTCACCGCCAGTTGTGGATGGACCTGTCGCTCAACCTAAAAGGTATTGTGGCGCAGCA
GCTTATTCCAACACCCGATGGGCAAGGGCGCAGGGCGTGTTTGGAAGTGTTTTTGAATACCCCTCTCGCTGCAGATTTAG
TGCGCAAGGGCGAAGTGCACGAGCTAAAAGAGTTGATGAAGCGCTCCACCGAGCAGGGCATGCAAACCTTCGATCAGGCG
CTATACGAGCTTTACGATTCGGGCGAAATTACCTACGAAGATGCGCTATCTCACGCGGATTCGCCCAACGACCTGCGCTT
GATGATCAAGCTGGGTTCGGAAAGCGATGCCGATTACCTCTCCAACGCTGCGGATGAGTTAAGTATCCAAGAGGATGAAA
GCAACCGCTCGCGCCGTTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(34-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q21EI5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

76.842

100

0.768
  pilU Acinetobacter baylyi ADP1

66.947

93.947

0.629
  pilU Vibrio cholerae strain A1552

55.556

97.105

0.539
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.188

90.789

0.392
  pilT Legionella pneumophila strain Lp02

40.896

88.158

0.361
  pilT Legionella pneumophila strain ERS1305867

40.896

88.158

0.361

Multiple sequence alignment