Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilA1   Type   Machinery gene
Locus tag   L21SP3_RS09750 Genome accession   NZ_CP019633
Coordinates   2417366..2417488 (-) Length   40 a.a.
NCBI ID   WP_077541016.1    Uniprot ID   A0A1Q2HRT5
Organism   Sedimentisphaera cyanobacteriorum strain L21-RPul-D3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2412366..2422488
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L21SP3_RS09730 (L21SP3_01932) - 2413397..2414815 (+) 1419 WP_077541009.1 sulfatase-like hydrolase/transferase -
  L21SP3_RS09740 (L21SP3_01934) - 2415553..2416458 (+) 906 WP_077541013.1 alpha/beta hydrolase -
  L21SP3_RS09745 (L21SP3_01935) - 2416613..2417365 (-) 753 WP_077541014.1 hypothetical protein -
  L21SP3_RS09750 (L21SP3_01936) pilA/pilA1 2417366..2417488 (-) 123 WP_077541016.1 type II secretion system protein Machinery gene
  L21SP3_RS09755 (L21SP3_01937) murC 2417724..2419148 (+) 1425 WP_077541017.1 UDP-N-acetylmuramate--L-alanine ligase -
  L21SP3_RS09760 (L21SP3_01938) - 2419160..2420872 (+) 1713 WP_077541019.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 40 a.a.        Molecular weight: 4398.50 Da        Isoelectric Point: 10.7687

>NTDB_id=217454 L21SP3_RS09750 WP_077541016.1 2417366..2417488(-) (pilA/pilA1) [Sedimentisphaera cyanobacteriorum strain L21-RPul-D3]
MNNKAFTLIELLVVISIIALLMAILMPALGRAREQAKMTV

Nucleotide


Download         Length: 123 bp        

>NTDB_id=217454 L21SP3_RS09750 WP_077541016.1 2417366..2417488(-) (pilA/pilA1) [Sedimentisphaera cyanobacteriorum strain L21-RPul-D3]
ATGAATAATAAAGCTTTTACGCTGATTGAGCTTTTGGTGGTAATCTCAATCATAGCCCTTTTGATGGCTATCTTGATGCC
CGCTCTGGGCAGGGCGAGGGAGCAGGCAAAAATGACCGTCTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Q2HRT5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilA4 Thermus thermophilus HB27

61.538

97.5

0.6
  pilA/pilA1 Synechocystis sp. PCC 6803

56.098

100

0.575
  pilV Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

57.576

82.5

0.475
  comGC Latilactobacillus sakei subsp. sakei 23K

54.545

82.5

0.45
  pilV Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.059

85

0.4
  comYC Streptococcus gordonii str. Challis substr. CH1

51.613

77.5

0.4
  pilA Ralstonia pseudosolanacearum GMI1000

44.444

90

0.4
  comGC Lactococcus lactis subsp. cremoris KW2

44.444

90

0.4
  comYC Streptococcus mutans UA159

44.444

90

0.4
  comYC Streptococcus mutans UA140

44.444

90

0.4
  pilE Neisseria gonorrhoeae MS11

41.667

90

0.375
  comYC Streptococcus suis isolate S10

44.118

85

0.375
  pilE Neisseria gonorrhoeae strain FA1090

41.667

90

0.375
  pilA/pilAI Pseudomonas stutzeri DSM 10701

60

62.5

0.375
  comGC/cglC Streptococcus pneumoniae TIGR4

48.387

77.5

0.375
  comGC/cglC Streptococcus mitis NCTC 12261

48.387

77.5

0.375
  comGC/cglC Streptococcus pneumoniae Rx1

48.387

77.5

0.375
  comGC/cglC Streptococcus pneumoniae D39

48.387

77.5

0.375
  comGC/cglC Streptococcus mitis SK321

48.387

77.5

0.375
  comGC/cglC Streptococcus pneumoniae R6

48.387

77.5

0.375
  fimT Acinetobacter baylyi ADP1

42.857

87.5

0.375
  pilA Vibrio campbellii strain DS40M4

42.857

87.5

0.375

Multiple sequence alignment