Detailed information    

experimental Experimentally validated

Overview

Name   fimT   Type   Machinery gene
Locus tag   ACIAD_RS03200 Genome accession   NC_005966
Coordinates   683552..684070 (-) Length   172 a.a.
NCBI ID   WP_004922618.1    Uniprot ID   A0A7G2SEP2
Organism   Acinetobacter baylyi ADP1     
Function   assembly of type IV pilus   
DNA binding and uptake

Function

The seven competence proteins, ComB, ComP, PilV, PilX and FimT are absolutely essential for natural transformation. These proteins are hydrophilic and are similar to pilins, the structural subunits of type IV pili (T4P). The disruption of comB, comP and the deletion of pilV, pilX or fimT resulted in complete transformation deficiencies.


Genomic Context

Location: 678552..689070
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACIAD_RS03170 (ACIAD0688) - 678808..679638 (+) 831 WP_004922636.1 copper resistance protein B -
  ACIAD_RS03175 (ACIAD0689) - 679693..680661 (+) 969 WP_004922633.1 cation transporter -
  ACIAD_RS03180 (ACIAD0691) - 680798..681118 (+) 321 WP_004922631.1 DMT family transporter -
  ACIAD_RS03185 (ACIAD0692) - 681134..681625 (+) 492 WP_004922629.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  ACIAD_RS03190 (ACIAD0693) - 681683..682129 (-) 447 WP_004922626.1 VOC family protein -
  ACIAD_RS03195 (ACIAD0694) - 682261..683436 (-) 1176 WP_004922622.1 thiolase family protein -
  ACIAD_RS03200 (ACIAD0695) fimT 683552..684070 (-) 519 WP_004922618.1 GspH/FimT family pseudopilin Machinery gene
  ACIAD_RS03205 (ACIAD0697) omp38 684455..685522 (+) 1068 WP_004922616.1 outer membrane protein Omp38 -
  ACIAD_RS16965 - 685634..685795 (-) 162 WP_004922613.1 hypothetical protein -
  ACIAD_RS03210 (ACIAD0700) - 686192..687655 (-) 1464 WP_004922610.1 amino acid permease -
  ACIAD_RS03215 (ACIAD0701) - 687674..688690 (-) 1017 WP_004922607.1 class I SAM-dependent methyltransferase -

Sequence

Protein


Download         Length: 172 a.a.        Molecular weight: 19634.53 Da        Isoelectric Point: 8.6371

>NTDB_id=1005 ACIAD_RS03200 WP_004922618.1 683552..684070(-) (fimT) [Acinetobacter baylyi ADP1]
MYNNCFGFTLIEFITVSAIIAILVSLGLPAYHRHMAAQEARTIPHILTIHIQKAKSEAVMYRHNVIICPSHDTQTCSDDW
SNGLIMFLDRNRNRERDLDEKILSATQWNTRYGSLHWRGTLHANNLMFKAGTGLPIASNGSFYYCSEKTANKKLVLSRMG
HIRHEQVNDCTL

Nucleotide


Download         Length: 519 bp        

>NTDB_id=1005 ACIAD_RS03200 WP_004922618.1 683552..684070(-) (fimT) [Acinetobacter baylyi ADP1]
ATGTATAACAATTGTTTTGGTTTCACTTTAATCGAATTTATCACTGTCAGTGCAATTATAGCCATTTTAGTGAGCTTAGG
TCTTCCCGCATATCATCGTCATATGGCGGCTCAAGAAGCGCGTACTATTCCTCATATACTCACAATTCACATACAAAAAG
CAAAATCTGAAGCGGTGATGTATCGACATAACGTGATTATCTGTCCAAGTCATGATACACAAACTTGTTCTGATGACTGG
AGCAATGGATTAATCATGTTTCTGGACCGAAACCGTAATCGTGAACGTGATTTAGATGAAAAAATTTTATCTGCTACTCA
ATGGAATACTCGTTATGGTTCACTTCACTGGAGAGGCACCTTACACGCAAACAACCTGATGTTTAAAGCTGGTACAGGTT
TACCAATTGCGTCAAATGGCAGTTTTTATTATTGCTCTGAGAAAACTGCTAATAAAAAACTTGTGCTGAGCCGTATGGGA
CATATTCGGCATGAACAGGTCAACGATTGCACTTTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(3-26)

Type II transport protein GspH (GspH)

(51-159)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G2SEP2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Beate Averhoff et al. (2021) Natural transformation in Gram-negative bacteria thriving in extreme environments: from genes and genomes to proteins, structures and regulation. Extremophiles : Life Under Extreme Conditions 25(5-6):425-436. [PMID: 34542714]
[2] Colleen G Leong et al. (2017) The role of core and accessory type IV pilus genes in natural transformation and twitching motility in the bacterium Acinetobacter baylyi. PloS One 12(8):e0182139. [PMID: 28771515]