Detailed information    

experimental Experimentally validated

Overview

Name   pilV   Type   Machinery gene
Locus tag   DR_RS06345 Genome accession   NC_001263
Coordinates   1240130..1240594 (+) Length   154 a.a.
NCBI ID   WP_010887875.1    Uniprot ID   Q9RUZ8
Organism   Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539     
Function   assembly of type IV pilus   
DNA binding and uptake

Function

We identified the factors (PilQ, PilD, type IV pilins, PilB, PilT, ComEC-ComEA, and ComF) involved in DNA uptake and DNA translocation across the external and cytoplasmic membranes and showed that the DNA-uptake machinery is similar to that described in the Gram negative bacterium Vibrio cholerae.


Genomic Context

Location: 1235130..1245594
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DR_RS06335 (DR_1230) - 1236986..1237741 (-) 756 WP_027480203.1 DUF4388 domain-containing protein -
  DR_RS06340 (DR_1231) - 1237875..1240133 (+) 2259 WP_162177751.1 hypothetical protein -
  DR_RS06345 (DR_1232) pilV 1240130..1240594 (+) 465 WP_010887875.1 type II secretion system protein Machinery gene
  DR_RS06350 (DR_1233) - 1240595..1241155 (+) 561 WP_010887876.1 type II secretion system protein -
  DR_RS06355 (DR_1234) - 1241176..1242036 (+) 861 WP_034350728.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  DR_RS06360 (DR_1235) - 1242098..1243822 (+) 1725 WP_027480202.1 dynamin family protein -
  DR_RS06365 (DR_1236) - 1243898..1244809 (+) 912 WP_034350720.1 cation diffusion facilitator family transporter -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 16402.98 Da        Isoelectric Point: 9.7879

>NTDB_id=1308 DR_RS06345 WP_010887875.1 1240130..1240594(+) (pilV) [Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539]
MKDRGQSGMTLIELLVVIAIIGILMGLFGWNLVRSIRAAELREAATQVAVDLRQARSQAQRGSRDIVVVLPGSTGGTTYR
FDTDVKTIPNNVQLICKSNCGTGTTTNITYQAPYGELGAIGSIYTVRSPMNGIGDYEIRIVGVTGKVILAKAGS

Nucleotide


Download         Length: 465 bp        

>NTDB_id=1308 DR_RS06345 WP_010887875.1 1240130..1240594(+) (pilV) [Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539]
ATGAAAGATCGTGGACAATCGGGGATGACTCTTATTGAACTGCTGGTTGTCATAGCGATCATCGGTATTTTGATGGGTCT
GTTCGGCTGGAATCTTGTGCGAAGCATTCGGGCTGCCGAGCTGCGGGAAGCGGCGACTCAGGTGGCGGTGGATCTACGCC
AGGCGCGCTCGCAGGCGCAGCGGGGCAGCAGAGATATTGTTGTCGTGTTGCCGGGGAGTACAGGCGGAACCACCTATAGG
TTCGACACGGACGTTAAAACCATTCCCAACAATGTGCAGCTGATCTGCAAATCGAATTGCGGCACCGGCACGACGACGAA
CATCACTTATCAGGCACCGTACGGCGAATTGGGCGCTATAGGGAGCATCTATACCGTCAGGAGCCCCATGAACGGAATTG
GCGATTATGAAATCAGAATCGTGGGCGTAACCGGCAAAGTCATTCTTGCTAAAGCGGGGTCCTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(4-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9RUZ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Solenne Ithurbide et al. (2020) Natural Transformation in Deinococcus radiodurans: A Genetic Analysis Reveals the Major Roles of DprA, DdrB, RecA, RecF, and RecO Proteins. Frontiers in Microbiology 11:1253. [PMID: 32625182]