Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   BIZ42_RS14005 Genome accession   NZ_CP017483
Coordinates   3180850..3181887 (+) Length   345 a.a.
NCBI ID   WP_019185385.1    Uniprot ID   A0A498C434
Organism   Stenotrophomonas sp. LM091     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3175850..3186887
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BIZ42_RS13980 (BIZ42_13980) - 3176496..3177869 (-) 1374 WP_070426769.1 MFS transporter -
  BIZ42_RS13985 (BIZ42_13985) soxR 3177960..3178394 (+) 435 WP_172838051.1 redox-sensitive transcriptional activator SoxR -
  BIZ42_RS13990 (BIZ42_13990) - 3178459..3179061 (-) 603 WP_019185382.1 short chain dehydrogenase -
  BIZ42_RS13995 (BIZ42_13995) proC 3179212..3180033 (-) 822 WP_070426770.1 pyrroline-5-carboxylate reductase -
  BIZ42_RS14000 (BIZ42_14000) - 3180061..3180741 (-) 681 WP_070426771.1 YggS family pyridoxal phosphate-dependent enzyme -
  BIZ42_RS14005 (BIZ42_14005) pilT 3180850..3181887 (+) 1038 WP_019185385.1 type IV pilus twitching motility protein PilT Machinery gene
  BIZ42_RS14010 (BIZ42_14010) pilU 3181939..3183075 (+) 1137 WP_019185386.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  BIZ42_RS14015 (BIZ42_14015) - 3183187..3183750 (+) 564 WP_070426772.1 hypothetical protein -
  BIZ42_RS14020 (BIZ42_14020) - 3184028..3185010 (+) 983 Protein_2772 aldo/keto reductase -
  BIZ42_RS14025 (BIZ42_14025) - 3185114..3185935 (-) 822 WP_070426773.1 alpha/beta hydrolase -
  BIZ42_RS14030 (BIZ42_14030) - 3186140..3186846 (+) 707 Protein_2774 YitT family protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38601.38 Da        Isoelectric Point: 6.5779

>NTDB_id=198717 BIZ42_RS14005 WP_019185385.1 3180850..3181887(+) (pilT) [Stenotrophomonas sp. LM091]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLACPPIFRELIDQPQGLILVTGPTGSGKSTTLAAMIDHINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGQQMGMMTLDQHL
QDLVKRSLITRNQAREYAKDKRIFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=198717 BIZ42_RS14005 WP_019185385.1 3180850..3181887(+) (pilT) [Stenotrophomonas sp. LM091]
ATGGATATCGCCGAACTGTTGGCGTTTTCCGTAAAGAACAAAGCGTCGGATCTTCATCTGTCGGCCGGCCTGCCGCCGAT
GATCCGCGTTGACGGCGATGTCCGTCGCATCAATATTCCGGCCCTGGACCACAAGCAGGTCCACGCGCTGGTGTACGACA
TCATGTCGGACAAGCAGCGTCGCGATTACGAAGAGTTCCTCGAGGTCGATTTCTCGTTCGAGATTCCCTCGCTGGCGCGC
TTCCGTGTCAACGCCTTCAACCAGAACCGCGGTGCGGGTGCGGTGTTCCGTACCATTCCCTCCGAGGTGCTCACCCTGGA
AGACCTGGCCTGCCCGCCGATCTTCCGCGAGCTGATCGATCAGCCGCAAGGGCTGATCCTGGTCACCGGCCCGACCGGTT
CGGGCAAGTCGACCACGCTGGCGGCGATGATCGACCACATCAACAAGAACGAATACGGCCACATCCTTACCGTCGAGGAC
CCGATCGAATTCGTGCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAGTGCATCGCGATACGCACGGCTTCAATGA
AGCGCTGCGCTCGGCACTGCGTGAGGATCCGGACATCATCCTGGTCGGCGAACTGCGCGATCTGGAAACCATCCGCCTGG
CGCTGACCGCCGCGGAAACGGGCCACCTGGTATTCGGCACGCTGCACACCAGCTCGGCCGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCCGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCCGAGTCGCTGCGCGCGGTGATCTCCCAGGC
CCTGCTGAAGAAGGTCGGTGGTGGGCGTACGGCTGCGTGGGAAATCATGGTCGGCACGCCGGCCATCCGCAACCTGATCC
GCGAAGACAAGGTCGCGCAGATGTACTCGGCGATCCAGACCGGCCAGCAGATGGGCATGATGACCCTGGACCAGCACCTG
CAGGACCTGGTCAAGCGCAGCCTGATCACCCGCAACCAGGCACGCGAGTACGCCAAGGACAAGCGGATTTTCGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A498C434

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.942

99.71

0.797
  pilT Acinetobacter baumannii D1279779

79.942

99.71

0.797
  pilT Acinetobacter baumannii strain A118

79.942

99.71

0.797
  pilT Pseudomonas stutzeri DSM 10701

78.488

99.71

0.783
  pilT Acinetobacter baylyi ADP1

78.198

99.71

0.78
  pilT Pseudomonas aeruginosa PAK

78.761

98.261

0.774
  pilT Legionella pneumophila strain Lp02

74.709

99.71

0.745
  pilT Legionella pneumophila strain ERS1305867

74.709

99.71

0.745
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.821

95.362

0.675
  pilT Vibrio cholerae strain A1552

70.821

95.362

0.675
  pilT Neisseria meningitidis 8013

65.689

98.841

0.649
  pilT Neisseria gonorrhoeae MS11

65.396

98.841

0.646
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.855

100

0.499
  pilU Acinetobacter baylyi ADP1

40.588

98.551

0.4
  pilU Pseudomonas stutzeri DSM 10701

40.413

98.261

0.397
  pilU Vibrio cholerae strain A1552

40.299

97.101

0.391

Multiple sequence alignment