Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   BIZ42_RS14010 Genome accession   NZ_CP017483
Coordinates   3181939..3183075 (+) Length   378 a.a.
NCBI ID   WP_019185386.1    Uniprot ID   -
Organism   Stenotrophomonas sp. LM091     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3176939..3188075
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BIZ42_RS13985 (BIZ42_13985) soxR 3177960..3178394 (+) 435 WP_172838051.1 redox-sensitive transcriptional activator SoxR -
  BIZ42_RS13990 (BIZ42_13990) - 3178459..3179061 (-) 603 WP_019185382.1 short chain dehydrogenase -
  BIZ42_RS13995 (BIZ42_13995) proC 3179212..3180033 (-) 822 WP_070426770.1 pyrroline-5-carboxylate reductase -
  BIZ42_RS14000 (BIZ42_14000) - 3180061..3180741 (-) 681 WP_070426771.1 YggS family pyridoxal phosphate-dependent enzyme -
  BIZ42_RS14005 (BIZ42_14005) pilT 3180850..3181887 (+) 1038 WP_019185385.1 type IV pilus twitching motility protein PilT Machinery gene
  BIZ42_RS14010 (BIZ42_14010) pilU 3181939..3183075 (+) 1137 WP_019185386.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  BIZ42_RS14015 (BIZ42_14015) - 3183187..3183750 (+) 564 WP_070426772.1 hypothetical protein -
  BIZ42_RS14020 (BIZ42_14020) - 3184028..3185010 (+) 983 Protein_2772 aldo/keto reductase -
  BIZ42_RS14025 (BIZ42_14025) - 3185114..3185935 (-) 822 WP_070426773.1 alpha/beta hydrolase -
  BIZ42_RS14030 (BIZ42_14030) - 3186140..3186846 (+) 707 Protein_2774 YitT family protein -
  BIZ42_RS14035 (BIZ42_14035) - 3186857..3187108 (-) 252 WP_156785748.1 hypothetical protein -
  BIZ42_RS14040 (BIZ42_14040) - 3187280..3187816 (-) 537 WP_019185392.1 DNA-3-methyladenine glycosylase I -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42165.53 Da        Isoelectric Point: 6.8286

>NTDB_id=198718 BIZ42_RS14010 WP_019185386.1 3181939..3183075(+) (pilU) [Stenotrophomonas sp. LM091]
MSTTTIDFTSFLKLMAHQKASDLFITAGMPPAIKVNGKISPITQTPLTPQQSRDLVLNVMTPAQREEFEKTHECNFAIGL
SGVGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPIVKTLAMTKRGIILFVGATGTGKSTSLAAMIGYRNHNSTGHI
ITIEDPIEFVHKHEGCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQ
AMDRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPSPDGKTRKVAMEIMLGTPLVQDYIRDGEIHKLKEVMKDSVQLGMR
TFDQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDARTLSQGLDGVEISEIR

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=198718 BIZ42_RS14010 WP_019185386.1 3181939..3183075(+) (pilU) [Stenotrophomonas sp. LM091]
ATGAGCACCACCACCATCGACTTCACCTCGTTCCTCAAGCTGATGGCGCACCAGAAGGCCTCGGACCTGTTCATCACCGC
GGGCATGCCGCCGGCGATCAAGGTCAACGGCAAGATCTCGCCGATCACCCAGACACCGTTGACGCCGCAGCAGAGCCGCG
ACCTGGTGTTGAACGTGATGACGCCGGCGCAGCGCGAAGAGTTCGAAAAGACCCACGAATGCAACTTCGCCATCGGCCTG
TCCGGTGTCGGCCGCTTCCGCGTGAGCTGCTTCTACCAGCGCAACCAGGTCGGCATGGTGCTGCGCCGGATCGAAACGCG
CATTCCCACCGTGGAAGAGCTGAGCCTGCCGCCGATCGTCAAGACGCTGGCGATGACCAAGCGCGGCATCATCCTGTTCG
TCGGCGCGACCGGTACCGGTAAATCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCACAACTCGACCGGCCACATC
ATCACCATCGAAGACCCGATCGAATTCGTGCACAAGCACGAAGGCTGCATCATCACCCAGCGCGAAGTCGGCATCGATAC
CGACAGCTGGGAGGCGGCGCTGAAGAACACCCTGCGCCAGGCCCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCG
AGGGCATGGACCACGCCATCGCTTTTGCTGAGACCGGCCACCTGGTGCTGTGCACCCTGCACGCCAACAACGCCAACCAG
GCGATGGACCGCATCATCAACTTCTTCCCGGAAGACCGTCGCAACCAGCTGCTGATGGATCTCTCGCTGAACCTCAAGGG
CGTGGTCGCCCAGCAGCTGATCCCCTCGCCGGATGGCAAGACGCGCAAGGTCGCGATGGAAATCATGCTGGGTACGCCGC
TGGTGCAGGATTACATCCGCGACGGTGAGATCCACAAGCTCAAGGAAGTGATGAAGGACTCGGTCCAGCTGGGCATGCGC
ACCTTCGACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAGATCAGCTACGAAGACGCCCTGCGCTACGCCGATTCGCA
GAATGAAGTACGCCTGCGCATCAAGCTCTCGCAGGGCGGCGACGCACGCACGCTGTCGCAGGGGCTGGACGGGGTGGAGA
TCTCCGAGATCCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.575

97.09

0.656
  pilU Acinetobacter baylyi ADP1

63.585

94.444

0.601
  pilU Vibrio cholerae strain A1552

53.371

94.18

0.503
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.737

94.709

0.405
  pilT Acinetobacter baumannii strain A118

40.407

91.005

0.368
  pilT Acinetobacter nosocomialis M2

40.407

91.005

0.368
  pilT Acinetobacter baumannii D1279779

40.407

91.005

0.368
  pilT Pseudomonas aeruginosa PAK

40.896

88.624

0.362
  pilT Legionella pneumophila strain Lp02

40.896

88.624

0.362
  pilT Legionella pneumophila strain ERS1305867

40.896

88.624

0.362

Multiple sequence alignment